APOD Antibody - #DF7987

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Product: APOD Antibody
Catalog: DF7987
Description: Rabbit polyclonal antibody to APOD
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 30 kDa; 21kD(Calculated).
Uniprot: P05090
RRID: AB_2841371

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
APOD Antibody detects endogenous levels of total APOD.
RRID:
AB_2841371
Cite Format: Affinity Biosciences Cat# DF7987, RRID:AB_2841371.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

APO D; Apo-D; ApoD; APOD protein; APOD_HUMAN; Apolipoprotein D; ApolipoproteinD;

Immunogens

Immunogen:
Uniprot:

P05090(APOD_HUMAN) >>Visit HPA database.

Gene(ID):
APOD(347)
Expression:
P05090 APOD_HUMAN:

Expressed in liver, intestine, pancreas, kidney, placenta, adrenal, spleen, fetal brain tissue and tears.

Sequence:
MVMLLLLLSALAGLFGAAEGQAFHLGKCPNPPVQENFDVNKYLGRWYEIEKIPTTFENGRCIQANYSLMENGKIKVLNQELRADGTVNQIEGEATPVNLTEPAKLEVKFSWFMPSAPYWILATDYENYALVYSCTCIIQLFHVDFAWILARNPNLPPETVDSLKNILTSNNIDVKKMTVTDQVNCPKLS

PTMs - P05090 As Substrate

Site PTM Type Enzyme
N65 N-Glycosylation
N98 N-Glycosylation

Research Backgrounds

Function:

APOD occurs in the macromolecular complex with lecithin-cholesterol acyltransferase. It is probably involved in the transport and binding of bilin. Appears to be able to transport a variety of ligands in a number of different contexts.

PTMs:

N-glycosylatd. N-glycan heterogeneity at Asn-65: Hex5HexNAc4 (major) and Hex6HexNAc5 (minor); at Asn-98: Hex5HexNAc4 (minor), dHex1Hex5HexNAc4 (major), dHex1Hex6HexNAc5 (minor) and dHex1Hex7HexNAc6 (minor).

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in liver, intestine, pancreas, kidney, placenta, adrenal, spleen, fetal brain tissue and tears.

Subunit Structure:

Homodimer. In plasma, also exists as a disulfide-linked heterodimer with APOA2.

Family&Domains:

Belongs to the calycin superfamily. Lipocalin family.

References

1). Dual functions of microRNA-17 in maintaining cartilage homeostasis and protection against osteoarthritis. Nature Communications, 2022 (PubMed: 35508470) [IF=16.6]

Application: IF/ICC    Species: Mouse    Sample: articular cartilage

Fig. 5 scRNA-seq analysis identified three subsets of chondrocytes. a Medial femoral condyle tissue from the region enclosed by the dotted lines was isolated for cell dissociation. n = 9 mice. Scale bar, 250 μm. b Putative chondrocyte population (171 cells) and unsupervised clustering identified clusters C1, C2, and C3 in cartilage. c Heatmap showing the expression of the top 20 identified significantly differentially expressed genes in clusters C1, C2, and C3. d Immunofluorescence staining of representative markers identified by scRNA-seq in articular cartilage. n = 3 independent experiments. All scale bars, 100 μm. e GO analysis and representative genes for clusters C1, C2, and C3.

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