FSCN1 Antibody - #DF7988

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Product: FSCN1 Antibody
Catalog: DF7988
Description: Rabbit polyclonal antibody to FSCN1
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.: 54 kDa; 55kD(Calculated).
Uniprot: Q16658
RRID: AB_2841372

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(88%), Sheep(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
FSCN1 Antibody detects endogenous levels of total FSCN1.
RRID:
AB_2841372
Cite Format: Affinity Biosciences Cat# DF7988, RRID:AB_2841372.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

55 kDa actin bundling protein; 55 kDa actin-bundling protein; Actin bundling protein; actin bundling protein, 55-KD; FAN 1; FAN1; Fascin 1; Fascin actin bundling protein 1; Fascin; Fascin homolog 1 actin bundling protein (Strongylocentrotus purpuratus); Fascin homolog 1; Fascin, sea urchin, homolog of, 1; Fascin1; FLJ38511; FSCN 1; FSCN1; FSCN1_HUMAN; HSN; p55; Singed (Drosophila) like (sea urchin fascin homolog like); Singed drosophila homolog like; Singed like (fascin homolog sea urchin) (Drosophila); Singed like (fascin homolog sea urchin); Singed like protein; Singed, drosophila, homolog of; Singed-like protein; SNL; Strongylocentrotus purpuratus;

Immunogens

Immunogen:
Uniprot:

Q16658(FSCN1_HUMAN) >>Visit HPA database.

Gene(ID):
FSCN1(6624)
Expression:
Q16658 FSCN1_HUMAN:

Ubiquitous.

Sequence:
MTANGTAEAVQIQFGLINCGNKYLTAEAFGFKVNASASSLKKKQIWTLEQPPDEAGSAAVCLRSHLGRYLAADKDGNVTCEREVPGPDCRFLIVAHDDGRWSLQSEAHRRYFGGTEDRLSCFAQTVSPAEKWSVHIAMHPQVNIYSVTRKRYAHLSARPADEIAVDRDVPWGVDSLITLAFQDQRYSVQTADHRFLRHDGRLVARPEPATGYTLEFRSGKVAFRDCEGRYLAPSGPSGTLKAGKATKVGKDELFALEQSCAQVVLQAANERNVSTRQGMDLSANQDEETDQETFQLEIDRDTKKCAFRTHTGKYWTLTATGGVQSTASSKNASCYFDIEWRDRRITLRASNGKFVTSKKNGQLAASVETAGDSELFLMKLINRPIIVFRGEHGFIGCRKVTGTLDANRSSYDVFQLEFNDGAYNIKDSTGKYWTVGSDSAVTSSGDTPVDFFFEFCDYNKVAIKVGGRYLKGDHAGVLKASAETVDPASLWEY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Horse
88
Xenopus
78
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16658 As Substrate

Site PTM Type Enzyme
T2 Acetylation
Y23 Phosphorylation
S38 Phosphorylation
S39 Phosphorylation P17252 (PRKCA) , Q05655 (PRKCD)
K41 Acetylation
K41 Ubiquitination
K42 Ubiquitination
K43 Acetylation
K43 Ubiquitination
Y69 Phosphorylation
K74 Acetylation
K74 Ubiquitination
T79 Phosphorylation
C80 S-Nitrosylation
R118 Methylation
S120 Phosphorylation
S127 Phosphorylation
Y145 Phosphorylation
S146 Phosphorylation
T148 Phosphorylation
R217 Methylation
S218 Phosphorylation
S234 Phosphorylation
S237 Phosphorylation
T239 Phosphorylation
K241 Ubiquitination
K247 Ubiquitination
K250 Acetylation
K250 Ubiquitination
S274 Phosphorylation
K304 Acetylation
K313 Ubiquitination
Y314 Phosphorylation
S333 Phosphorylation
K353 Methylation
K353 Ubiquitination
K359 Ubiquitination
C397 S-Nitrosylation
K399 Sumoylation
K399 Ubiquitination
T403 Phosphorylation
Y411 Phosphorylation
K426 Ubiquitination
Y432 Phosphorylation
T447 Phosphorylation
Y458 Phosphorylation
K464 Ubiquitination
K471 Acetylation
K471 Ubiquitination
K479 Ubiquitination
T484 Phosphorylation

Research Backgrounds

Function:

Actin-binding protein that contains 2 major actin binding sites. Organizes filamentous actin into parallel bundles. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration. Mediates reorganization of the actin cytoskeleton and axon growth cone collapse in response to NGF.

PTMs:

Phosphorylation at Ser-39 inhibits actin-binding. Phosphorylation is required for the reorganization of the actin cytoskeleton in response to NGF.

Subcellular Location:

Cytoplasm>Cytosol. Cytoplasm>Cell cortex. Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Stress fiber. Cell projection>Filopodium. Cell projection>Invadopodium. Cell projection>Microvillus. Cell junction.
Note: Colocalized with RUFY3 and F-actin at filipodia of the axonal growth cone. Colocalized with DBN1 and F-actin at the transitional domain of the axonal growth cone (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts with RUFY3 (via N-terminus); the interaction induces neuron axon development (By similarity). Interacts with NGFR (By similarity). Associates with CTNNB1 (By similarity). Interacts with PLXNB3.

Family&Domains:

Composed of four fascin beta-trefoil domains.

Belongs to the fascin family.

Research Fields

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

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