Product: ARFRP1 Antibody
Catalog: DF7990
Description: Rabbit polyclonal antibody to ARFRP1
Application: WB
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 23 kDa; 23kD(Calculated).
Uniprot: Q13795
RRID: AB_2841374

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(86%), Bovine(86%), Horse(100%), Sheep(86%), Dog(100%)
Clonality:
Polyclonal
Specificity:
ARFRP1 Antibody detects endogenous levels of total ARFRP1.
RRID:
AB_2841374
Cite Format: Affinity Biosciences Cat# DF7990, RRID:AB_2841374.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ADP ribosylation factor related protein 1; ADP-ribosylation factor-related protein 1; ARF related protein 1; ARF related protein; ARF-related protein 1; ARFRP_HUMAN; ARFRP1; ARL 18; ARL18; ARP 1; ARP; ARP1; Helicase like protein NHL; MGC6837; SCG10 like protein;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q13795 ARFRP_HUMAN:

Found in most tissues.

Sequence:
MYTLLSGLYKYMFQKDEYCILILGLDNAGKTTFLEQSKTRFNKNYKGMSLSKITTTVGLNIGTVDVGKARLMFWDLGGQEELQSLWDKYYAECHGVIYVIDSTDEERLAESKQAFEKVVTSEALCGVPVLVLANKQDVETCLSIPDIKTAFSDCTSKIGRRDCLTQACSALTGKGVREGIEWMVKCVVRNVHRPPRQRDIT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Pig
86
Bovine
86
Sheep
86
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13795 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K38 Ubiquitination
K112 Ubiquitination
S156 Phosphorylation
K157 Ubiquitination
T165 Phosphorylation
K174 Ubiquitination

Research Backgrounds

Function:

Trans-Golgi-associated GTPase that regulates protein sorting. Controls the targeting of ARL1 and its effector to the trans-Golgi. Required for the lipidation of chylomicrons in the intestine and required for VLDL lipidation in the liver.

Subcellular Location:

Golgi apparatus. Golgi apparatus>trans-Golgi network.
Note: Located in the trans-Golgi in the GTP-bound active state.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Found in most tissues.

Subunit Structure:

Interacts with SYS1.

Family&Domains:

Belongs to the small GTPase superfamily. Arf family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.