OLIG2 Antibody - #DF8004

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Product: OLIG2 Antibody
Catalog: DF8004
Description: Rabbit polyclonal antibody to OLIG2
Application: WB IHC
Cited expt.: WB, IHC
Reactivity: Human, Monkey
Prediction: Pig, Dog, Chicken, Xenopus
Mol.Wt.: 32 kDa; 32kD(Calculated).
Uniprot: Q13516
RRID: AB_2841383

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Monkey
Prediction:
Pig(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
OLIG2 Antibody detects endogenous levels of total OLIG2.
RRID:
AB_2841383
Cite Format: Affinity Biosciences Cat# DF8004, RRID:AB_2841383.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Basic domain helix loop helix protein class B 1; Basic helix loop helix protein class B 1; BHLHB; bHLHB1; bHLHe19; Class B basic helix loop helix protein 1; Class B basic helix-loop-helix protein 1; class E basic helix loop helix protein 19; Class E basic helix-loop-helix protein 19; Human protein kinase C binding protein RACK17; Olig2; OLIG2_HUMAN; Oligo2; Oligodendrocyte lineage transcription factor 2; Oligodendrocyte specific bHLH transcription factor 2; Oligodendrocyte transcription factor 2; OTTHUMP00000067569; OTTHUMP00000067570; PRKCBP2; Protein kinase C binding protein 2; Protein kinase C binding protein RACK17; Protein kinase C-binding protein 2; Protein kinase C-binding protein RACK17; RACK17;

Immunogens

Immunogen:

A synthesized peptide derived from human OLIG2, corresponding to a region within the internal amino acids.

Uniprot:

Q13516(OLIG2_HUMAN) >>Visit HPA database.

Gene(ID):
OLIG2(10215)
Expression:
Q13516 OLIG2_HUMAN:

Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable.

Sequence:
MDSDASLVSSRPSSPEPDDLFLPARSKGSSGSAFTGGTVSSSTPSDCPPELSAELRGAMGSAGAHPGDKLGGSGFKSSSSSTSSSTSSAAASSTKKDKKQMTEPELQQLRLKINSRERKRMHDLNIAMDGLREVMPYAHGPSVRKLSKIATLLLARNYILMLTNSLEEMKRLVSEIYGGHHAGFHPSACGGLAHSAPLPAATAHPAAAAHAAHHPAVHHPILPPAAAAAAAAAAAAAVSSASLPGSGLPSVGSIRPPHGLLKSPSAAAAAPLGGGGGGSGASGGFQHWGGMPCPCSMCQVPPPHHHVSAMGAGSLPRLTSDAK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Xenopus
100
Chicken
100
Zebrafish
64
Horse
0
Bovine
0
Sheep
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Functions together with ZNF488 to promote oligodendrocyte differentiation. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.

Subcellular Location:

Nucleus. Cytoplasm.
Note: The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable.

Family&Domains:

The bHLH is essential for interaction with NKX2-2.

References

1). ACT001 inhibited CD133 transcription by targeting and inducing Olig2 ubiquitination degradation. Oncogenesis, 2023 (PubMed: 36990974) [IF=5.9]

Application: WB    Species: human    Sample: lung cancer tissues

Fig. 1: Olig2 was overexpressed in lung cancer tissues. a The representative pictures of immunohistochemical staining of Olig2 in paraffin-embedded human lung cancer tissues and non-lung cancer tissues. b Statistical analysis of Olig2 immunohistochemical staining of lung cancer tissues and non-lung cancer tissues, n = 8. c Western blot analysis of Olig2 expression in lung cancer tissues and non-lung cancer tissues, n = 8. d Statistical analysis of Olig2 expression in lung cancer tissues and non-lung cancer tissues, values were normalized to β-actin, n = 8. The data were presented as the mean ± SD, *p 

Application: IHC    Species: human    Sample: lung cancer tissues

Fig. 2: Olig2 promoted lung cancer cell stemness. a Average correlations between Olig2 expression and the functional states in glioma. b Representative images and number of tumorspheres with Olig2 knockdown or overexpression in NCI-H820 cells, n = 6. c Representative images and number of NCI-H820 migrated cells with Olig2 knockdown or overexpression, n = 3. d The bioluminescence imaging of lungs with intravenous injection of NCI-H820-luci cells into BAL B/c nude mice for 7 days, n = 5. e Statistical analysis of bioluminescence intensity in lungs, n = 5. f Representative HE staining images of lung and liver metastasis. g The picture of xenograft tumors derived from NCI-H820 cells with intra-tumoral injected Olig2 siRNA or si-NC in lipofectamine 2000. h Xenograft tumor growth curve of Olig2 siRNA or si-NC-transfected group, n = 4. i Xenograft tumor weight of Olig2 siRNA or si-NC-transfected group, n = 4. j Immunohistochemical staining of Olig2 and CD133 of xenograft tumor. k Western bolt analysis of Olig2, CD133, Nanog, and Oct4 expression in xenograft tumors. l Statistical analysis of Olig2, CD133, Nanog, and Oct4 expression in xenograft tumors, values were normalized to β-actin, n = 4. The data were presented as the mean ± SD, *p 

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