Product: GPA33 Antibody
Catalog: DF8049
Description: Rabbit polyclonal antibody to GPA33
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 36 kDa; 36kD(Calculated).
Uniprot: Q99795
RRID: AB_2841407

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(91%), Bovine(91%), Horse(82%), Sheep(91%), Rabbit(91%), Dog(91%)
Clonality:
Polyclonal
Specificity:
GPA33 Antibody detects endogenous levels of total GPA33.
RRID:
AB_2841407
Cite Format: Affinity Biosciences Cat# DF8049, RRID:AB_2841407.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

A33; Cell surface A33 antigen; Cell surface A33 antigen precursor; Glycoprotein A33 (transmembrane); Glycoprotein A33; Gpa33; GPA33_HUMAN; MGC129986; MGC129987; OTTHUMP00000032346; Transmembrane glycoprotein A33;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q99795 GPA33_HUMAN:

Expressed in normal gastrointestinal epithelium and in 95% of colon cancers.

Sequence:
MVGKMWPVLWTLCAVRVTVDAISVETPQDVLRASQGKSVTLPCTYHTSTSSREGLIQWDKLLLTHTERVVIWPFSNKNYIHGELYKNRVSISNNAEQSDASITIDQLTMADNGTYECSVSLMSDLEGNTKSRVRLLVLVPPSKPECGIEGETIIGNNIQLTCQSKEGSPTPQYSWKRYNILNQEQPLAQPASGQPVSLKNISTDTSGYYICTSSNEEGTQFCNITVAVRSPSMNVALYVGIAVGVVAALIIIGIIIYCCCCRGKDDNTEDKEDARPNREAYEEPPEQLRELSREREEEDDYRQEEQRSTGRESPDHLDQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Bovine
91
Sheep
91
Dog
91
Rabbit
91
Horse
82
Xenopus
70
Chicken
64
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99795 As Substrate

Site PTM Type Enzyme
S50 O-Glycosylation
N112 N-Glycosylation
S197 Phosphorylation
Y281 Phosphorylation
Y301 Phosphorylation
S313 Phosphorylation

Research Backgrounds

Function:

May play a role in cell-cell recognition and signaling.

PTMs:

N-glycosylated, contains approximately 8 kDa of N-linked carbohydrate.

Palmitoylated.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in normal gastrointestinal epithelium and in 95% of colon cancers.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.