EN1 Antibody | Affinity Biosciences

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IF/ICC

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EN1 Antibody - Western blot analysis of EN1 using RAW264.

EN1 Antibody - DF8055 staining A549 cells by IF/ICC.

Product:	EN1 Antibody
Catalog:	DF8055
Description:	Rabbit polyclonal antibody to EN1
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Rabbit, Chicken
Mol.Wt.:	40 kDa; 40kD(Calculated).
Uniprot:	Q05925
RRID:	AB_2841412

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Bovine(100%), Rabbit(89%), Chicken(88%)

Clonality:

Polyclonal

Specificity:

EN1 Antibody detects endogenous levels of total EN1.

RRID:

AB_2841412
Cite Format: Affinity Biosciences Cat# DF8055, RRID:AB_2841412.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

En 1; EN1; Engrailed 1; Engrailed; Engrailed homeobox 1; Engrailed homolog 1; HME1_HUMAN; Homeobox protein en-1; Homeobox protein engrailed 1; Homeobox protein engrailed-1; Hu-En-1; Mo-en.1; OTTHUMP00000162091;

Immunogens

Immunogen:

Uniprot:

Q05925(HME1_HUMAN) >>Visit HPA database.

Gene(ID):

EN1(2019)

Sequence:

Q05925 HME1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEEQQPEPKSQRDSALGAAAAATPGGLSLSLSPGASGSSGSGSDGDSVPVSPQPAPPSPPAAPCLPPLAHHPHLPPHPPPPPPQHLAAPAHQPQPAAQLHRTTNFFIDNILRPDFGCKKEQPPPQLLVAAAARGGAGGGGRVERDRGQTAAGRDPVHPLGTRAPGAASLLCAPDANCGPPDGSQPAAAGAGASKAGNPAAAAAAAAAAVAAAAAAAAAKPSDTGGGGSGGGAGSPGAQGTKYPEHGNPAILLMGSANGGPVVKTDSQQPLVWPAWVYCTRYSDRPSSGPRTRKLKKKKNEKEDKRPRTAFTAEQLQRLKAEFQANRYITEQRRQTLAQELSLNESQIKIWFQNKRAKIKKATGIKNGLALHLMAQGLYNHSTTTVQDKDESE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Rabbit

Chicken

Xenopus

Horse

Sheep

Dog

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q05925 As Substrate

Q05925

Site	PTM Type	Enzyme	Source
T149	Phosphorylation		Uniprot
S391	Phosphorylation		Uniprot

Research Backgrounds

Q05925_HME1_HUMAN

Subcellular Location:

Nucleus.

Family&Domains:

Belongs to the engrailed homeobox family.

References

1). A hybrid hydrogel composed of chitin and β-glucan for the effective management of wound healing and scarring. Biomaterials Science, 2022 (PubMed: 36073016) [IF=5.8]

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

EN1 Antibody - #DF8055