USP10 Antibody - #DF8061
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF8061, RRID:AB_2841417.
Fold/Unfold
Deubiquitinating enzyme 10; KIAA0190; MGC124997; MGC2621; Ubiquitin carboxyl terminal hydrolase 10; Ubiquitin carboxyl-terminal hydrolase 10; Ubiquitin specific peptidase 10; Ubiquitin specific processing protease 10; Ubiquitin specific protease 10; Ubiquitin thioesterase 10; Ubiquitin thiolesterase 10; Ubiquitin-specific-processing protease 10; UBP10_HUMAN; UBPO; USP 10; USP10;
Immunogens
- Q14694 UBP10_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MALHSPQYIFGDFSPDEFNQFFVTPRSSVELPPYSGTVLCGTQAVDKLPDGQEYQRIEFGVDEVIEPSDTLPRTPSYSISSTLNPQAPEFILGCTASKITPDGITKEASYGSIDCQYPGSALALDGSSNVEAEVLENDGVSGGLGQRERKKKKKRPPGYYSYLKDGGDDSISTEALVNGHANSAVPNSVSAEDAEFMGDMPPSVTPRTCNSPQNSTDSVSDIVPDSPFPGALGSDTRTAGQPEGGPGADFGQSCFPAEAGRDTLSRTAGAQPCVGTDTTENLGVANGQILESSGEGTATNGVELHTTESIDLDPTKPESASPPADGTGSASGTLPVSQPKSWASLFHDSKPSSSSPVAYVETKYSPPAISPLVSEKQVEVKEGLVPVSEDPVAIKIAELLENVTLIHKPVSLQPRGLINKGNWCYINATLQALVACPPMYHLMKFIPLYSKVQRPCTSTPMIDSFVRLMNEFTNMPVPPKPRQALGDKIVRDIRPGAAFEPTYIYRLLTVNKSSLSEKGRQEDAEEYLGFILNGLHEEMLNLKKLLSPSNEKLTISNGPKNHSVNEEEQEEQGEGSEDEWEQVGPRNKTSVTRQADFVQTPITGIFGGHIRSVVYQQSSKESATLQPFFTLQLDIQSDKIRTVQDALESLVARESVQGYTTKTKQEVEISRRVTLEKLPPVLVLHLKRFVYEKTGGCQKLIKNIEYPVDLEISKELLSPGVKNKNFKCHRTYRLFAVVYHHGNSATGGHYTTDVFQIGLNGWLRIDDQTVKVINQYQVVKPTAERTAYLLYYRRVDLL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q14694 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Ubiquitination | Uniprot | ||
| A2 | Acetylation | Uniprot | |
| S5 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| T24 | Phosphorylation | Uniprot | |
| C40 | S-Nitrosylation | Uniprot | |
| T42 | Phosphorylation | Q13315 (ATM) | Uniprot |
| Y54 | Phosphorylation | Uniprot | |
| S68 | Phosphorylation | Uniprot | |
| T70 | Phosphorylation | Uniprot | |
| T74 | Phosphorylation | Uniprot | |
| S76 | Phosphorylation | Uniprot | |
| Y77 | Phosphorylation | Uniprot | |
| S81 | Phosphorylation | Uniprot | |
| T82 | Phosphorylation | Uniprot | |
| T95 | Phosphorylation | Uniprot | |
| T100 | Phosphorylation | Uniprot | |
| Y110 | Phosphorylation | Uniprot | |
| K154 | Ubiquitination | Uniprot | |
| Y159 | Phosphorylation | Uniprot | |
| Y160 | Phosphorylation | Uniprot | |
| S161 | Phosphorylation | Uniprot | |
| Y162 | Phosphorylation | Uniprot | |
| S203 | Phosphorylation | Uniprot | |
| T205 | Phosphorylation | Uniprot | |
| T208 | Phosphorylation | Uniprot | |
| S211 | Phosphorylation | Uniprot | |
| S215 | Phosphorylation | Uniprot | |
| T216 | Phosphorylation | Uniprot | |
| S218 | Phosphorylation | Uniprot | |
| S220 | Phosphorylation | Uniprot | |
| S226 | Phosphorylation | Uniprot | |
| C254 | S-Nitrosylation | Uniprot | |
| T263 | Phosphorylation | Uniprot | |
| S265 | Phosphorylation | Uniprot | |
| S309 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| T327 | Phosphorylation | Uniprot | |
| S329 | Phosphorylation | Uniprot | |
| S337 | Phosphorylation | Q13315 (ATM) | Uniprot |
| S341 | Phosphorylation | Uniprot | |
| S355 | Phosphorylation | Uniprot | |
| Y359 | Phosphorylation | Uniprot | |
| Y364 | Phosphorylation | Uniprot | |
| S365 | Phosphorylation | Uniprot | |
| S370 | Phosphorylation | Uniprot | |
| K376 | Ubiquitination | Uniprot | |
| K381 | Ubiquitination | Uniprot | |
| K395 | Ubiquitination | Uniprot | |
| K408 | Ubiquitination | Uniprot | |
| K488 | Ubiquitination | Uniprot | |
| Y503 | Phosphorylation | Uniprot | |
| K512 | Ubiquitination | Uniprot | |
| S516 | Phosphorylation | Uniprot | |
| K518 | Ubiquitination | Uniprot | |
| Y527 | Phosphorylation | Uniprot | |
| K544 | Ubiquitination | Uniprot | |
| S547 | Phosphorylation | Uniprot | |
| S549 | Phosphorylation | Uniprot | |
| K552 | Ubiquitination | Uniprot | |
| S556 | Phosphorylation | Uniprot | |
| S563 | Phosphorylation | Uniprot | |
| S576 | Phosphorylation | Uniprot | |
| T600 | Phosphorylation | Uniprot | |
| Y659 | Phosphorylation | Uniprot | |
| K662 | Ubiquitination | Uniprot | |
| K664 | Ubiquitination | Uniprot | |
| S670 | Phosphorylation | Uniprot | |
| T674 | Phosphorylation | Uniprot | |
| K677 | Ubiquitination | Uniprot | |
| Y691 | Phosphorylation | Uniprot | |
| K693 | Ubiquitination | Uniprot | |
| T694 | Phosphorylation | Uniprot | |
| K699 | Ubiquitination | Uniprot | |
| K702 | Ubiquitination | Uniprot | |
| S713 | Phosphorylation | Uniprot | |
| K714 | Acetylation | Uniprot | |
| K714 | Ubiquitination | Uniprot | |
| S718 | Phosphorylation | Uniprot | |
| K722 | Ubiquitination | Uniprot | |
| K780 | Ubiquitination | Uniprot |
Research Backgrounds
Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13. Does not deubiquitinate MDM2. Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling. Involved in a TANK-dependent negative feedback response to attenuate NF-kappaB activation via deubiquitinating IKBKG or TRAF6 in response to interleukin-1-beta (IL1B) stimulation or upon DNA damage. Deubiquitinates TBX21 leading to its stabilization.
Phosphorylated by ATM following DNA damage, leading to stablization and translocation it to the nucleus.
Ubiquitinated. Deubiquitinated by USP13.
Cytoplasm. Nucleus. Early endosome.
Note: Cytoplasmic in normal conditions (PubMed:20096447). After DNA damage, translocates to the nucleus following phosphorylation by ATM (PubMed:20096447).
Widely expressed.
Found in a deubiquitination complex with TANK, USP10 and ZC3H12A; this complex inhibits genotoxic stress- or interleukin-1-beta (IL1B)-mediated NF-kappaB activation by promoting IKBKG or TRAF6 deubiquitination. Interacts with IKBKG; this interaction increases in response to DNA damage. Interacts with TANK; this interaction increases in response to DNA damage. Interacts with TRAF6; this interaction increases in response to DNA damage. Interacts with ZC3H12A; this interaction increases in response to DNA damage. Interacts with G3BP1 (via NTF2-like domain) and G3BP2 (via NTF2-like domain). Interacts with p53/TP53. Interacts with SNX3. Interacts with CFTR. Interacts with TBX21.
Belongs to the peptidase C19 family. USP10 subfamily.
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