EBF1 Antibody | Affinity Biosciences

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EBF1 Antibody - Western blot analysis of EBF1 using HepG2 whole cell lysates.

Product:	EBF1 Antibody
Catalog:	DF8074
Description:	Rabbit polyclonal antibody to EBF1
Application:	WB
Reactivity:	Human
Prediction:	Rabbit
Mol.Wt.:	64 kDa; 64kD(Calculated).
Uniprot:	Q9UH73
RRID:	AB_2841428

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Rabbit(100%)

Clonality:

Polyclonal

Specificity:

EBF1 Antibody detects endogenous levels of total EBF1.

RRID:

AB_2841428
Cite Format: Affinity Biosciences Cat# DF8074, RRID:AB_2841428.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

COE1; COE1_HUMAN; Collier, Olf and EBF transcription factor 1; Early B cell factor 1; Early B-cell factor; EBF; Ebf1; O/E 1; O/E-1; OE 1; OE-1; OE1; OLF1; olfactory neuronal transcription factor 1; Transcription factor COE1;

Immunogens

Immunogen:

Uniprot:

Q9UH73(COE1_HUMAN) >>Visit HPA database.

Gene(ID):

EBF1(1879)

Sequence:

Q9UH73 COE1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MFGIQESIQRSGSSMKEEPLGSGMNAVRTWMQGAGVLDANTAAQSGVGLARAHFEKQPPSNLRKSNFFHFVLALYDRQGQPVEIERTAFVGFVEKEKEANSEKTNNGIHYRLQLLYSNGIRTEQDFYVRLIDSMTKQAIVYEGQDKNPEMCRVLLTHEIMCSRCCDKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKNAGNPRDMRRFQVVVSTTVNVDGHVLAVSDNMFVHNNSKHGRRARRLDPSEGTPSYLEHATPCIKAISPSEGWTTGGATVIIIGDNFFDGLQVIFGTMLVWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGTPGRFIYTALNEPTIDYGFQRLQKVIPRHPGDPERLPKEVILKRAADLVEALYGMPHNNQEIILKRAADIAEALYSVPRNHNQLPALANTSVHAGMMGVNSFSGQLAVNVSEASQATNQGFTRNSSSVSPHGYVPSTTPQQTNYNSVTTSMNGYGSAAMSNLGGSPTFLNGSAANSPYAIVPSSPTMASSTSLPSNCSSSSGIFSFSPANMVSAVKQKSAFAPVVRPQTSPPPTCTSTNGNSLQAISGMIVPPM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Pig

Bovine

Dog

Horse

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UH73 As Substrate

Q9UH73

Site	PTM Type	Source
M1	Acetylation	Uniprot
K16	Sumoylation	Uniprot
K56	Ubiquitination	Uniprot
K330	Ubiquitination	Uniprot
K361	Ubiquitination	Uniprot
Y412	Phosphorylation	Uniprot

Research Backgrounds

Q9UH73_COE1_HUMAN

Function:

Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'.

Subcellular Location:

Nucleus.

Subunit Structure:

Forms either a homodimer or a heterodimer with a related family member (By similarity). Interacts with ZNF423 and ZNF521, leading to prevent EBF1 to bind DNA and activate target genes.

Family&Domains:

Belongs to the COE family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

EBF1 Antibody - #DF8074