ING3 Antibody - #DF8079
| Product: | ING3 Antibody |
| Catalog: | DF8079 |
| Description: | Rabbit polyclonal antibody to ING3 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 47 kDa.; 47kD(Calculated). |
| Uniprot: | Q9NXR8 |
| RRID: | AB_2841432 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF8079, RRID:AB_2841432.
Fold/Unfold
1300013A07Rik; Eaf 4; Eaf4; FLJ20089; ING 2; ING 3; ING2; ING3; ING3_HUMAN; Inhibitor of growth family member 3; Inhibitor of growth protein 3; MEAF4; p47 ING 3; p47 ING3; p47 regulator protein; p47ING3; p47ING3 protein;
Immunogens
Expressed in brain, heart, kidney, liver, lung, ovaries, placenta, prostate, skeletal muscle, small intestine, spleen, testis and thymus.
- Q9NXR8 ING3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLYLEDYLEMIEQLPMDLRDRFTEMREMDLQVQNAMDQLEQRVSEFFMNAKKNKPEWREEQMASIKKDYYKALEDADEKVQLANQIYDLVDRHLRKLDQELAKFKMELEADNAGITEILERRSLELDTPSQPVNNHHAHSHTPVEKRKYNPTSHHTTTDHIPEKKFKSEALLSTLTSDASKENTLGCRNNNSTASSNNAYNVNSSQPLGSYNIGSLSSGTGAGAITMAAAQAVQATAQMKEGRRTSSLKASYEAFKNNDFQLGKEFSMARETVGYSSSSALMTTLTQNASSSAADSRSGRKSKNNNKSSSQQSSSSSSSSSLSSCSSSSTVVQEISQQTTVVPESDSNSQVDWTYDPNEPRYCICNQVSYGEMVGCDNQDCPIEWFHYGCVGLTEAPKGKWYCPQCTAAMKRRGSRHK
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NXR8 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R19 | Methylation | Uniprot | |
| S64 | Phosphorylation | Uniprot | |
| K71 | Ubiquitination | Uniprot | |
| K79 | Ubiquitination | Uniprot | |
| Y87 | Phosphorylation | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| K103 | Ubiquitination | Uniprot | |
| K164 | Acetylation | Uniprot | |
| K167 | Sumoylation | Uniprot | |
| K167 | Ubiquitination | Uniprot | |
| S168 | Phosphorylation | Uniprot | |
| S173 | Phosphorylation | Uniprot | |
| K181 | Acetylation | Uniprot | |
| K181 | Ubiquitination | Uniprot | |
| S246 | Phosphorylation | Uniprot | |
| S247 | Phosphorylation | Uniprot | |
| K256 | Ubiquitination | Uniprot | |
| K264 | Acetylation | Uniprot | |
| K264 | Ubiquitination | Uniprot | |
| S309 | Phosphorylation | Uniprot | |
| S314 | Phosphorylation | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| S316 | Phosphorylation | Uniprot | |
| S317 | Phosphorylation | Uniprot | |
| S318 | Phosphorylation | Uniprot | |
| S326 | Phosphorylation | Uniprot | |
| K400 | Ubiquitination | Uniprot | |
| K411 | Ubiquitination | Uniprot |
Research Backgrounds
Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AZ1 from the nucleosome.
Nucleus.
Expressed in brain, heart, kidney, liver, lung, ovaries, placenta, prostate, skeletal muscle, small intestine, spleen, testis and thymus.
Interacts with H3K4me3 and to a lesser extent with H3K4me2. Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. The NuA4 complex interacts with MYC and the adenovirus E1A protein. HTATTIP/TIP60, EPC1, and ING3 together constitute a minimal HAT complex termed Piccolo NuA4. Component of a SWR1-like complex.
The PHD-type zinc finger mediates the binding to H3K4me3.
Belongs to the ING family.
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