ING4 Antibody - #DF8085
| Product: | ING4 Antibody |
| Catalog: | DF8085 |
| Description: | Rabbit polyclonal antibody to ING4 |
| Application: | WB |
| Reactivity: | Human |
| Prediction: | Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 29 kDa; 29kD(Calculated). |
| Uniprot: | Q9UNL4 |
| RRID: | AB_2841437 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF8085, RRID:AB_2841437.
Fold/Unfold
Brain my036 protein; Candidate tumor suppressor p33 ING 1 homolog; Candidate tumor suppressor p33 ING1 homolog; D6Wsu147e; D6Xrf92; ING 1 like protein; ING 4; ING1 like protein; ING4; ING4_HUMAN; Inhibitor of growth family member 4; Inhibitor of growth family member 4 long isoform; Inhibitor of growth protein 4; MGC12557; my036; p29 ING 4; p29 ING4; p29ING4;
Immunogens
- Q9UNL4 ING4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAGMYLEHYLDSIENLPFELQRNFQLMRDLDQRTEDLKAEIDKLATEYMSSARSLSSEEKLALLKQIQEAYGKCKEFGDDKVQLAMQTYEMVDKHIRRLDTDLARFEADLKEKQIESSDYDSSSSKGKKKGRTQKEKKAARARSKGKNSDEEAPKTAQKKLKLVRTSPEYGMPSVTFGSVHPSDVLDMPVDPNEPTYCLCHQVSYGEMIGCDNPDCSIEWFHFACVGLTTKPRGKWFCPRCSQERKKK
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UNL4 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K44 | Ubiquitination | Uniprot | |
| T47 | Phosphorylation | Uniprot | |
| Y49 | Phosphorylation | Uniprot | |
| S51 | Phosphorylation | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| K74 | Ubiquitination | Uniprot | |
| K95 | Ubiquitination | Uniprot | |
| K112 | Acetylation | Uniprot | |
| K114 | Acetylation | Uniprot | |
| S123 | Phosphorylation | Uniprot | |
| K127 | Acetylation | Uniprot | |
| K129 | Acetylation | Uniprot | |
| K130 | Acetylation | Uniprot | |
| K131 | Acetylation | Uniprot | |
| K136 | Acetylation | Uniprot | |
| K139 | Acetylation | Uniprot | |
| K146 | Acetylation | Uniprot | |
| K148 | Acetylation | Uniprot | |
| S150 | Phosphorylation | Uniprot | |
| K156 | Acetylation | Uniprot | |
| K160 | Acetylation | Uniprot | |
| K161 | Acetylation | Uniprot | |
| K236 | Acetylation | Uniprot |
Research Backgrounds
Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may function in DNA replication. May inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. Can suppress brain tumor angiogenesis through transcriptional repression of RELA/NFKB3 target genes when complexed with RELA. May also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. Represses hypoxia inducible factor's (HIF) activity by interacting with HIF prolyl hydroxylase 2 (EGLN1). Can enhance apoptosis induced by serum starvation in mammary epithelial cell line HC11 (By similarity).
Citrullination by PADI4 within the nuclear localization signal disrupts the interaction with p53 and increases susceptibility to degradation.
Nucleus.
Homodimer. Interacts with H3K4me3 and to a lesser extent with H3K4me2, the interaction augments HBO1 acetylation activity on H3 tails. Component of the HBO1 complex composed at least of ING4 or ING5, KAT7/HBO1, MEAF6, and one of JADE1, JADE2 and JADE3. Interacts with EP300, RELA and TP53; these interactions may be indirect. Interacts with EGLN1. Interacts with BCL2A1 (By similarity).
The PHD-type zinc finger mediates the binding to H3K4me3.
The N-terminal coiled-coil domain mediates homodimerization.
Belongs to the ING family.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.