GOLM1 Antibody - #DF8100

(1)   (3)  
Product: GOLM1 Antibody
Catalog: DF8100
Description: Rabbit polyclonal antibody to GOLM1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Dog
Mol.Wt.: 80 kDa; 45kD(Calculated).
Uniprot: Q8NBJ4
RRID: AB_2841445

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(90%), Bovine(90%), Sheep(100%), Dog(90%)
Clonality:
Polyclonal
Specificity:
GOLM1 Antibody detects endogenous levels of total GOLM1.
RRID:
AB_2841445
Cite Format: Affinity Biosciences Cat# DF8100, RRID:AB_2841445.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

bA379P1.3; C9orf155; Chromosome 9 open reading frame 155; Golgi membrane protein 1; Golgi membrane protein GP73; Golgi phosphoprotein 2; Golgi protein 73 kD; Golgi protein 73kD; GOLM 1; GOLM1; GOLM1_HUMAN; GOLPH 2; GOLPH2; GP 73; GP73; PSEC0257;

Immunogens

Immunogen:
Uniprot:

Q8NBJ4(GOLM1_HUMAN) >>Visit HPA database.

Gene(ID):
GOLM1(51280)
Expression:
Q8NBJ4 GOLM1_HUMAN:

Widely expressed. Highly expressed in colon, prostate, trachea and stomach. Expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. Predominantly expressed by cells of the epithelial lineage. Expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), it is strongly expressed in hepatocytes-derived syncytial giant cells. Constitutively expressed by biliary epithelial cells but not by hepatocytes.

Sequence:
MMGLGNGRRSMKSPPLVLAALVACIIVLGFNYWIASSRSVDLQTRIMELEGRVRRAAAERGAVELKKNEFQGELEKQREQLDKIQSSHNFQLESVNKLYQDEKAVLVNNITTGERLIRVLQDQLKTLQRNYGRLQQDVLQFQKNQTNLERKFSYDLSQCINQMKEVKEQCEERIEEVTKKGNEAVASRDLSENNDQRQQLQALSEPQPRLQAAGLPHTEVPQGKGNVLGNSKSQTPAPSSEVVLDSKRQVEKEETNEIQVVNEEPQRDRLPQEPGREQVVEDRPVGGRGFGGAGELGQTPQVQAALSVSQENPEMEGPERDQLVIPDGQEEEQEAAGEGRNQQKLRGEDDYNMDENEAESETDKQAALAGNDRNIDVFNVEDQKRDTINLLDQREKRNHTL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Sheep
100
Pig
90
Bovine
90
Dog
90
Rabbit
57
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8NBJ4 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K76 Ubiquitination
S86 Phosphorylation
N109 N-Glycosylation
T111 O-Glycosylation
K125 Ubiquitination
S153 Phosphorylation
K164 Ubiquitination
S187 Phosphorylation
T218 O-Glycosylation
S231 O-Glycosylation
S231 Phosphorylation
S233 O-Glycosylation
T235 O-Glycosylation
S239 O-Glycosylation
T255 Phosphorylation
S307 Phosphorylation
S309 Phosphorylation
Y351 Phosphorylation
S360 Phosphorylation
T362 Phosphorylation
K384 Acetylation

Research Backgrounds

Function:

Unknown. Cellular response protein to viral infection.

PTMs:

Glycosylated.

Phosphorylation sites are present in the extracellular medium.

Subcellular Location:

Golgi apparatus>cis-Golgi network membrane>Single-pass type II membrane protein.
Note: Early Golgi. Cycles via the cell surface and endosomes upon lumenal pH disruption.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Highly expressed in colon, prostate, trachea and stomach. Expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. Predominantly expressed by cells of the epithelial lineage. Expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), it is strongly expressed in hepatocytes-derived syncytial giant cells. Constitutively expressed by biliary epithelial cells but not by hepatocytes.

Subunit Structure:

Interacts with DYM.

Family&Domains:

Belongs to the GOLM1/CASC4 family.

References

1). GOLM1 is related to the inflammatory/immune nature of uveal melanoma and acts as a promising indicator for prognosis and immunotherapy response. Frontiers in Genetics, 2022 (PubMed: 36468024) [IF=3.7]

Application: IHC    Species: Human    Sample: tumor tissues

FIGURE 7 Correlation between GOLM1 expression and PD-1, PD-L1, CTLA4, and IFN-γ. (A) Immunohistochemistry assay revealed the protein expression of GOLM1, PD-1, PD-L1, CTLA4, and IFN-γ. (B,C) The expression difference of PD-1 among GOLM1-low (LEXP) and GOLM1-high (HEXP) subgroups (B) and the correlation between PD-1 and GOLM1 expression (C). (D,E) The expression difference of PD-L1 among the LEXP and HEXP groups (D) and the correlation between PD-L1 and GOLM1 expression (E). (F,G) The expression difference of CTLA4 among the LEXP and HEXP groups (F) and the correlation between CTLA4 and GOLM1 expression (G). (H,I) The expression difference of IFN-γ among the LEXP and HEXP groups (H) and the correlation between IFN-γ and GOLM1 expression (I).

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.