PYGO2 Antibody - #DF8107

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Product: PYGO2 Antibody
Catalog: DF8107
Description: Rabbit polyclonal antibody to PYGO2
Application: WB IF/ICC
Reactivity: Human
Prediction: Bovine, Horse, Dog
Mol.Wt.: 41 kDa; 41kD(Calculated).
Uniprot: Q9BRQ0
RRID: AB_2841450

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Bovine(100%), Horse(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
PYGO2 Antibody detects endogenous levels of total PYGO2.
RRID:
AB_2841450
Cite Format: Affinity Biosciences Cat# DF8107, RRID:AB_2841450.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

1190004M21Rik; FLJ33226; PP7910; PYGO2; Pygo2 protein; PYGO2_HUMAN; Pygopus 2; pygopus homolog 2 (Drosophila); Pygopus homolog 2;

Immunogens

Immunogen:
Uniprot:

Q9BRQ0(PYGO2_HUMAN) >>Visit HPA database.

Gene(ID):
PYGO2(90780)
Sequence:
MAASAPPPPDKLEGGGGPAPPPAPPSTGRKQGKAGLQMKSPEKKRRKSNTQGPAYSHLTEFAPPPTPMVDHLVASNPFEDDFGAPKVGVAAPPFLGSPVPFGGFRVQGGMAGQVPPGYSTGGGGGPQPLRRQPPPFPPNPMGPAFNMPPQGPGYPPPGNMNFPSQPFNQPLGQNFSPPSGQMMPGPVGGFGPMISPTMGQPPRAELGPPSLSQRFAQPGAPFGPSPLQRPGQGLPSLPPNTSPFPGPDPGFPGPGGEDGGKPLNPPASTAFPQEPHSGSPAAAVNGNQPSFPPNSSGRGGGTPDANSLAPPGKAGGGSGPQPPPGLVYPCGACRSEVNDDQDAILCEASCQKWFHRECTGMTESAYGLLTTEASAVWACDLCLKTKEIQSVYIREGMGQLVAANDG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Xenopus
67
Pig
0
Sheep
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BRQ0 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S26 Phosphorylation
T27 Phosphorylation
S40 Phosphorylation
S48 Phosphorylation P31749 (AKT1)
T50 Phosphorylation
T59 Phosphorylation
T66 Phosphorylation
S97 Phosphorylation
R105 Methylation
T120 Phosphorylation
R130 Methylation
R131 Methylation
T302 Phosphorylation
S307 Phosphorylation
S318 Phosphorylation
K386 Ubiquitination

Research Backgrounds

Function:

Involved in signal transduction through the Wnt pathway.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Binds to BCL9 via the PHD-type zinc finger motif, and thereby becomes part of the nuclear beta-catenin/TCF complex.

References

1). EFNA4 deletion suppresses the migration, invasion, stemness, and angiogenesis of gastric cancer cells through the inactivation of Pygo2/Wnt signaling. Histology and histopathology, 2024 (PubMed: 38953488) [IF=2.5]

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