TRIM69 Antibody | Affinity Biosciences

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TRIM69 Antibody - Western blot analysis of TRIM69 using HeLa whole cell lysates.

Product:	TRIM69 Antibody
Catalog:	DF8161
Description:	Rabbit polyclonal antibody to TRIM69
Application:	WB
Reactivity:	Human
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit
Mol.Wt.:	57 kDa; 57kD(Calculated).
Uniprot:	Q86WT6
RRID:	AB_2841485

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%)

Clonality:

Polyclonal

Specificity:

TRIM69 Antibody detects endogenous levels of total TRIM69.

RRID:

AB_2841485
Cite Format: Affinity Biosciences Cat# DF8161, RRID:AB_2841485.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

E3 ubiquitin protein ligase TRIM69; HSD34; RFP like domain containing protein trimless; RFP-like domain-containing protein trimless; RING finger protein 36; RNF36; TRI69_HUMAN; Trif; Trim69; Trimless; Tripartite motif containing 69; tripartite motif containing protein 69; tripartite motif protein 69; Tripartite motif-containing protein 69;

Immunogens

Immunogen:

Uniprot:

Q86WT6(TRI69_HUMAN) >>Visit HPA database.

Gene(ID):

TRIM69(140691)

Sequence:

Q86WT6 TRI69_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEVSTNPSSNIDPGDYVEMNDSITHLPSKVVIQDITMELHCPLCNDWFRDPLMLSCGHNFCEACIQDFWRLQAKETFCPECKMLCQYNNCTFNPVLDKLVEKIKKLPLLKGHPQCPEHGENLKLFSKPDGKLICFQCKDARLSVGQSKEFLQISDAVHFFTEELAIQQGQLETTLKELQTLRNMQKEAIAAHKENKLHLQQHVSMEFLKLHQFLHSKEKDILTELREEGKALNEEMELNLSQLQEQCLLAKDMLVSIQAKTEQQNSFDFLKDITTLLHSLEQGMKVLATRELISRKLNLGQYKGPIQYMVWREMQDTLCPGLSPLTLDPKTAHPNLVLSKSQTSVWHGDIKKIMPDDPERFDSSVAVLGSRGFTSGKWYWEVEVAKKTKWTVGVVRESIIRKGSCPLTPEQGFWLLRLRNQTDLKALDLPSFSLTLTNNLDKVGIYLDYEGGQLSFYNAKTMTHIYTFSNTFMEKLYPYFCPCLNDGGENKEPLHILHPQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Rabbit

100

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q86WT6 As Substrate

Q86WT6

Site	PTM Type	Source
S204	Phosphorylation	Uniprot
T289	Phosphorylation	Uniprot
S294	Phosphorylation	Uniprot
T317	Phosphorylation	Uniprot
S323	Phosphorylation	Uniprot

Research Backgrounds

Q86WT6_TRI69_HUMAN

Function:

May have E3 ubiquitin-protein ligase activity. May play a role in apoptosis.

PTMs:

Phosphorylated. Phosphorylation is necessary for nuclear localization.

Subcellular Location:

Cytoplasm. Nucleus. Nucleus speckle.

Subunit Structure:

Interacts with PML.

Family&Domains:

The RING-type zinc finger domain is responsible for E3 ubiquitin ligase activity and for nuclear localization and aggregation.

Belongs to the TRIM/RBCC family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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TRIM69 Antibody - #DF8161