ING2 Antibody | Affinity Biosciences

1/1

ING2 Antibody - Western blot analysis ING2 using HeLa whole cell lysates.

Product:	ING2 Antibody
Catalog:	DF8240
Description:	Rabbit polyclonal antibody to ING2
Application:	WB
Reactivity:	Human
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	33 kDa; 33kD(Calculated).
Uniprot:	Q9H160
RRID:	AB_2841536

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(100%), Bovine(88%), Horse(100%), Sheep(88%), Rabbit(100%), Dog(88%), Chicken(88%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

ING2 Antibody detects endogenous levels of total ING2.

RRID:

AB_2841536
Cite Format: Affinity Biosciences Cat# DF8240, RRID:AB_2841536.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ING 1 like tumor suppressor protein; ING 1L; ING 2; ING1 like tumor suppressor protein; ING1L; ING1Lp; ING2; ING2_HUMAN; Inhibitor of growth 1 like; Inhibitor of growth 1 like protein; Inhibitor of growth 1-like protein; Inhibitor of growth family member 1 like; Inhibitor of growth family member 2; Inhibitor of growth protein 2; p32; p33 ING2; p33ING2;

Immunogens

Immunogen:

Uniprot:

Q9H160(ING2_HUMAN) >>Visit HPA database.

Gene(ID):

ING2(3622)

Expression:

Q9H160 ING2_HUMAN:

Widely expressed. Higher expressed in colon-cancer tumor than in normal colon tissues.

Sequence:

Q9H160 ING2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLGQQQQQLYSSAALLTGERSRLLTCYVQDYLECVESLPHDMQRNVSVLRELDNKYQETLKEIDDVYEKYKKEDDLNQKKRLQQLLQRALINSQELGDEKIQIVTQMLELVENRARQMELHSQCFQDPAESERASDKAKMDSSQPERSSRRPRRQRTSESRDLCHMANGIEDCDDQPPKEKKSKSAKKKKRSKAKQEREASPVEFAIDPNEPTYCLCNQVSYGEMIGCDNEQCPIEWFHFSCVSLTYKPKGKWYCPKCRGDNEKTMDKSTEKTKKDRRSR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Xenopus

100

Rabbit

100

Bovine

Sheep

Dog

Chicken

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H160 As Substrate

Q9H160

Site	PTM Type	Source
K61	Ubiquitination	Uniprot
K69	Ubiquitination	Uniprot
Y70	Phosphorylation	Uniprot
K257	Ubiquitination	Uniprot
K264	Acetylation	Uniprot
K275	Acetylation	Uniprot

Research Backgrounds

Q9H160_ING2_HUMAN

Function:

Seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. Component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. ING2 activity seems to be modulated by binding to phosphoinositides (PtdInsPs).

PTMs:

Sumoylation enhances its association with SIN3A and is required for binding to some target gene promoters, this is the case for TMEM71.

Subcellular Location:

Nucleus.
Note: Predominantly nuclear. Localized to chromatin and nuclear matrix. Upon reduced PtdIns(5)P levels seems to be released from chromatin and, at least partially, translocated to the cytoplasm.

Tissue Specificity:

Widely expressed. Higher expressed in colon-cancer tumor than in normal colon tissues.

Subunit Structure:

Interacts with H3K4me3 and to a lesser extent with H3K4me2. Component of a mSin3A-like complex at least consisting of SIN3A, HDAC1, HDAC2, RBBP4/RbAp48, RBBP7/RbAp46, SAP30 and ING2.

Family&Domains:

The PHD-type zinc finger mediates the binding to H3K4me3.

The polybasic region (PBR) is responsive to the binding to phosphoinositides (PtdInsPs), including phosphatidylinositol 5-phosphate (PtdIns(5)P).

Belongs to the ING family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

ING2 Antibody - #DF8240