PPP1R9A Antibody - #DF8257

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Product: PPP1R9A Antibody
Catalog: DF8257
Description: Rabbit polyclonal antibody to PPP1R9A
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 180 kDa; 123kD(Calculated).
Uniprot: Q9ULJ8
RRID: AB_2841547

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Horse(100%), Sheep(89%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
PPP1R9A Antibody detects endogenous levels of total PPP1R9A.
RRID:
AB_2841547
Cite Format: Affinity Biosciences Cat# DF8257, RRID:AB_2841547.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ20068; KIAA1222; NEB1_HUMAN; Neurabin I; Neurabin-1; Neurabin-I; Neurabin1; NeurabinI; Neural tissue specific F actin binding protein I; Neural tissue-specific F-actin-binding protein I; NRB 1; NRB I; NRB1; NRBI; Ppp1r9a; Protein phosphatase 1 regulatory (inhibitor) subunit 9A; Protein phosphatase 1 regulatory subunit 9A;

Immunogens

Immunogen:
Uniprot:

Q9ULJ8(NEB1_HUMAN) >>Visit HPA database.

Gene(ID):
PPP1R9A(55607)
Sequence:
MLKTESSGERTTLRSASPHRNAYRTEFQALKSTFDKPKSDGEQKTKEGEGSQQSRGRKYGSNVNRIKNLFMQMGMEPNENAAVIAKTRGKGGHSSPQRRMKPKEFLEKTDGSVVKLESSVSERISRFDTMYDGPSYSKFTETRKMFERSVHESGQNNRYSPKKEKAGGSEPQDEWGGSKSNRGSTDSLDSLSSRTEAVSPTVSQLSAVFENTDSPSAIISEKAENNEYSVTGHYPLNLPSVTVTNLDTFGHLKDSNSWPPSNKRGVDTEDAHKSNATPVPEVASKSTSLASIPGEEIQQSKEPEDSTSNQQTPDSIDKDGPEEPCAESKAMPKSEIPSPQSQLLEDAEANLVGREAAKQQRKELAGGDFTSPDASASSCGKEVPEDSNNFDGSHVYMHSDYNVYRVRSRYNSDWGETGTEQDEEEDSDENSYYQPDMEYSEIVGLPEEEEIPANRKIKFSSAPIKVFNTYSNEDYDRRNDEVDPVAASAEYELEKRVEKLELFPVELEKDEDGLGISIIGMGVGADAGLEKLGIFVKTVTEGGAAQRDGRIQVNDQIVEVDGISLVGVTQNFAATVLRNTKGNVRFVIGREKPGQVSEVAQLISQTLEQERRQRELLEQHYAQYDADDDETGEYATDEEEDEVGPVLPGSDMAIEVFELPENEDMFSPSELDTSKLSHKFKELQIKHAVTEAEIQKLKTKLQAAENEKVRWELEKTQLQQNIEENKERMLKLESYWIEAQTLCHTVNEHLKETQSQYQALEKKYNKAKKLIKDFQQKELDFIKRQEAERKKIEDLEKAHLVEVQGLQVRIRDLEAEVFRLLKQNGTQVNNNNNIFERRTSLGEVSKGDTMENLDGKQTSCQDGLSQDLNEAVPETERLDSKALKTRAQLSVKNRRQRPSRTRLYDSVSSTDGEDSLERKNFTFNDDFSPSSTSSADLSGLGAEPKTPGLSQSLALSSDESLDMIDDEILDDGQSPKHSQCQNRAVQEWSVQQVSHWLMSLNLEQYVSEFSAQNITGEQLLQLDGNKLKALGMTASQDRAVVKKKLKEMKMSLEKARKAQEKMEKQREKLRRKEQEQMQRKSKKTEKMTSTTAEGAGEQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Rabbit
100
Sheep
89
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9ULJ8 As Substrate

Site PTM Type Enzyme
S119 Phosphorylation
Y131 Phosphorylation
S135 Phosphorylation
Y136 Phosphorylation
S137 Phosphorylation
S149 Phosphorylation
S153 Phosphorylation
S160 Phosphorylation
K162 Acetylation
S169 Phosphorylation
K179 Acetylation
T185 Phosphorylation
S187 Phosphorylation
S190 Phosphorylation
S199 Phosphorylation
T201 Phosphorylation
T212 Phosphorylation
S229 Phosphorylation
T277 Phosphorylation
S284 Phosphorylation
S291 Phosphorylation
S334 Phosphorylation
S338 Phosphorylation
S341 Phosphorylation
S371 Phosphorylation
S460 Phosphorylation P17612 (PRKACA)
S461 Phosphorylation
K681 Ubiquitination
K696 Ubiquitination
K726 Ubiquitination
K777 Ubiquitination
T839 Phosphorylation
S840 Phosphorylation
T849 Phosphorylation
S865 Phosphorylation
S890 Phosphorylation
S915 Phosphorylation
S974 Phosphorylation
K1028 Acetylation

Research Backgrounds

Function:

Binds to actin filaments (F-actin) and shows cross-linking activity. Binds along the sides of the F-actin. May be involved in neurite formation. Inhibits protein phosphatase 1-alpha activity (By similarity).

Subcellular Location:

Cytoplasm>Cytoskeleton. Cell junction>Synapse>Synaptosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Possibly exists as a homodimer, homotrimer or a homotetramer. Interacts with F-actin, protein phosphatase 1 (PP1), neurabin-2 and p70-S6K (By similarity).

Family&Domains:

Interacts with p70-S6K via its PDZ domain.

The PP1 binding region is natively unstructured, upon PP1 binding, it acquires structure, blocks a substrate-binding site, and restricts PP1 phosphatase specificity to a subset of substrates.

Restrictive clause

 

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