WAPAL Antibody - #DF8272

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Product: WAPAL Antibody
Catalog: DF8272
Description: Rabbit polyclonal antibody to WAPAL
Application: WB
Reactivity: Human
Prediction: Bovine, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 133 kDa; 133kD(Calculated).
Uniprot: Q7Z5K2
RRID: AB_2841561

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
WAPAL Antibody detects endogenous levels of total WAPAL.
RRID:
AB_2841561
Cite Format: Affinity Biosciences Cat# DF8272, RRID:AB_2841561.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FOE; Friend of EBNA2 (Epstein Barr virus nuclear protein 2); Friend of EBNA2 protein; KIAA0261; OTTHUMP00000020004; WAPAL; WAPL; WAPL_HUMAN; Wings apart like homolog; Wings apart like homolog (Drosophila); Wings apart-like protein homolog;

Immunogens

Immunogen:
Uniprot:

Q7Z5K2(WAPL_HUMAN) >>Visit HPA database.

Gene(ID):
WAPL(23063)
Expression:
Q7Z5K2 WAPL_HUMAN:

Isoform 1 is highly expressed in uterine cervix tumor. Isoform 2 is widely expressed with a high level in skeletal muscle and heart.

Sequence:
MTSRFGKTYSRKGGNGSSKFDEVFSNKRTTLSTKWGETTFMAKLGQKRPNFKPDIQEIPKKPKVEEESTGDPFGFDSDDESLPVSSKNLAQVKCSSYSESSEAAQLEEVTSVLEANSKISHVVVEDTVVSDKCFPLEDTLLGKEKSTNRIVEDDASISSCNKLITSDKVENFHEEHEKNSHHIHKNADDSTKKPNAETTVASEIKETNDTWNSQFGKRPESPSEISPIKGSVRTGLFEWDNDFEDIRSEDCILSLDSDPLLEMKDDDFKNRLENLNEAIEEDIVQSVLRPTNCRTYCRANKTKSSQGASNFDKLMDGTSQALAKANSESSKDGLNQAKKGGVSCGTSFRGTVGRTRDYTVLHPSCLSVCNVTIQDTMERSMDEFTASTPADLGEAGRLRKKADIATSKTTTRFRPSNTKSKKDVKLEFFGFEDHETGGDEGGSGSSNYKIKYFGFDDLSESEDDEDDDCQVERKTSKKRTKTAPSPSLQPPPESNDNSQDSQSGTNNAENLDFTEDLPGVPESVKKPINKQGDKSKENTRKIFSGPKRSPTKAVYNARHWNHPDSEELPGPPVVKPQSVTVRLSSKEPNQKDDGVFKAPAPPSKVIKTVTIPTQPYQDIVTALKCRREDKELYTVVQHVKHFNDVVEFGENQEFTDDIEYLLSGLKSTQPLNTRCLSVISLATKCAMPSFRMHLRAHGMVAMVFKTLDDSQHHQNLSLCTAALMYILSRDRLNMDLDRASLDLMIRLLELEQDASSAKLLNEKDMNKIKEKIRRLCETVHNKHLDLENITTGHLAMETLLSLTSKRAGDWFKEELRLLGGLDHIVDKVKECVDHLSRDEDEEKLVASLWGAERCLRVLESVTVHNPENQSYLIAYKDSQLIVSSAKALQHCEELIQQYNRAEDSICLADSKPLPHQNVTNHVGKAVEDCMRAIIGVLLNLTNDNEWGSTKTGEQDGLIGTALNCVLQVPKYLPQEQRFDIRVLGLGLLINLVEYSARNRHCLVNMETSCSFDSSICSGEGDDSLRIGGQVHAVQALVQLFLERERAAQLAESKTDELIKDAPTTQHDKSGEWQETSGEIQWVSTEKTDGTEEKHKKEEEDEELDLNKALQHAGKHMEDCIVASYTALLLGCLCQESPINVTTVREYLPEGDFSIMTEMLKKFLSFMNLTCAVGTTGQKSISRVIEYLEHC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
71
Pig
0
Horse
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q7Z5K2 As Substrate

Site PTM Type Enzyme
K12 Acetylation
K19 Acetylation
K27 Acetylation
K27 Ubiquitination
S77 Phosphorylation
S81 Phosphorylation
K93 Ubiquitination
C94 S-Nitrosylation
S98 Phosphorylation
S130 Phosphorylation
T147 Phosphorylation
S156 Phosphorylation
S158 Phosphorylation
S159 Phosphorylation
K168 Acetylation
S180 Phosphorylation
S190 Phosphorylation
T191 Phosphorylation
S202 Phosphorylation
K205 Ubiquitination
S221 Phosphorylation
S223 Phosphorylation
S226 Phosphorylation
S231 Phosphorylation
S254 Phosphorylation
S257 Phosphorylation
S286 Phosphorylation
T291 Phosphorylation
K303 Ubiquitination
S304 Phosphorylation
S305 Phosphorylation
K313 Ubiquitination
S347 Phosphorylation
T355 Phosphorylation
T359 Phosphorylation
S380 Phosphorylation
T385 Phosphorylation
S387 Phosphorylation
T388 Phosphorylation
K408 Ubiquitination
S420 Phosphorylation
T436 Phosphorylation
S443 Phosphorylation
S445 Phosphorylation
Y448 Phosphorylation
Y452 Phosphorylation
S459 Phosphorylation
S461 Phosphorylation
S487 Phosphorylation
S523 Phosphorylation
K541 Methylation
S544 Phosphorylation
S549 Phosphorylation
T551 Phosphorylation
Y555 Phosphorylation
K597 Ubiquitination
K604 Ubiquitination
Y616 Phosphorylation
K624 Ubiquitination
Y633 Phosphorylation
K763 Acetylation
K763 Ubiquitination
K812 Ubiquitination
K827 Ubiquitination
K829 Ubiquitination
K843 Ubiquitination
K886 Ubiquitination
S904 Phosphorylation
K911 Ubiquitination
K1059 Ubiquitination
T1063 Phosphorylation
T1064 Phosphorylation
S1069 Phosphorylation
T1075 Phosphorylation
S1076 Phosphorylation
K1107 Ubiquitination
S1153 Phosphorylation
K1178 Ubiquitination

Research Backgrounds

Function:

Regulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin. Involved in both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair.

Subcellular Location:

Nucleus.

Nucleus. Chromosome. Cytoplasm.
Note: Associates with chromatin through the cohesin complex during interphase. Released in the cytoplasm from nuclear envelope breakdown until anaphase, it reaccumulates in nucleus at telophase.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 1 is highly expressed in uterine cervix tumor. Isoform 2 is widely expressed with a high level in skeletal muscle and heart.

Subunit Structure:

Interacts with the cohesin complex throughout the cell cycle; interacts with both chromatin-bound and soluble pools of the complex. Interacts with RAD21; the interaction is direct. Interacts with PDS5A; the interaction is direct, cohesin-dependent and competitive with CDCA5/SORORIN. Interacts (via FGF motifs) with PDS5B; the interaction is direct. Interacts with a SMC1 protein (SMC1A or SMC1B) and SMC3.

(Microbial infection) Isoform 2 interacts with Epstein-Barr virus EBNA2.

Family&Domains:

Belongs to the WAPL family.

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