SIRT5 Antibody - #DF8294

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Product: SIRT5 Antibody
Catalog: DF8294
Description: Rabbit polyclonal antibody to SIRT5
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 34 kDa; 34kD(Calculated).
Uniprot: Q9NXA8
RRID: AB_2841579

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Bovine(100%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(92%), Chicken(92%)
Clonality:
Polyclonal
Specificity:
SIRT5 Antibody detects endogenous levels of total SIRT5.
RRID:
AB_2841579
Cite Format: Affinity Biosciences Cat# DF8294, RRID:AB_2841579.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

NAD dependent deacetylase sirtuin 5; NAD dependent lysine demalonylase and desuccinylase sirtuin 5 mitochondrial; NAD dependent protein deacylase sirtuin 5 mitochondrial; NAD-dependent protein deacylase sirtuin-5, mitochondrial; Regulatory protein SIR2 homolog 5; Silent mating type information regulation 2 S.cerevisiae homolog 5; Sir2 like 5; SIR2-like protein 5; SIR2L5; SIR5_HUMAN; Sirt5; Sirtuin 5; Sirtuin type 5;

Immunogens

Immunogen:
Uniprot:

Q9NXA8(SIR5_HUMAN) >>Visit HPA database.

Gene(ID):
SIRT5(23408)
Expression:
Q9NXA8 SIR5_HUMAN:

Widely expressed.

Sequence:
MRPLQIVPSRLISQLYCGLKPPASTRNQICLKMARPSSSMADFRKFFAKAKHIVIISGAGVSAESGVPTFRGAGGYWRKWQAQDLATPLAFAHNPSRVWEFYHYRREVMGSKEPNAGHRAIAECETRLGKQGRRVVVITQNIDELHRKAGTKNLLEIHGSLFKTRCTSCGVVAENYKSPICPALSGKGAPEPGTQDASIPVEKLPRCEEAGCGGLLRPHVVWFGENLDPAILEEVDRELAHCDLCLVVGTSSVVYPAAMFAPQVAARGVPVAEFNTETTPATNRFRFHFQGPCGTTLPEALACHENETVS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Rabbit
100
Pig
92
Sheep
92
Dog
92
Chicken
92
Xenopus
67
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NXA8 As Substrate

Site PTM Type Enzyme
Y76 Phosphorylation
T87 Phosphorylation
S160 Phosphorylation
K203 Ubiquitination

Research Backgrounds

Function:

NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species. Activates SHMT2 by mediating its desuccinylation. Modulates ketogenesis through the desuccinylation and activation of HMGCS2 (By similarity). Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX.

Subcellular Location:

Mitochondrion matrix. Mitochondrion intermembrane space. Cytoplasm>Cytosol. Nucleus.
Note: Mainly mitochondrial. Also present extramitochondrially, with a fraction present in the cytosol and very small amounts also detected in the nucleus.

Cytoplasm. Mitochondrion.

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Subunit Structure:

Interacts with CPS1 (By similarity). Interacts with PCCA. Monomer. Homodimer. Forms homodimers upon suramin binding.

Family&Domains:

In contrast to class I sirtuins, class III sirtuins have only weak deacetylase activity. Difference in substrate specificity is probably due to a larger hydrophobic pocket with 2 residues (Tyr-102 and Arg-105) that bind to malonylated and succinylated substrates and define the specificity (PubMed:22076378).

Belongs to the sirtuin family. Class III subfamily.

References

1). SIRT5-mediated SDHA desuccinylation promotes clear cell renal cell carcinoma tumorigenesis. FREE RADICAL BIOLOGY AND MEDICINE, 2019 (PubMed: 30703481) [IF=7.1]

Application: WB    Species: Human    Sample: ccRCC

Fig. 6. SIRT5 promotes ccRCC cell proliferation by interacting with and desuccinylating SDHA. (A–B) ACHN cells were transfected with scramble shRNA or SIRT5 shRNA plasmid, SDHA was precipitated and its succinylation level was probed by anti-pan-succinylation antibody. SIRT5 silencing led to hypersuccinylation of SDHA in ccRCC cells and increased activity of SDHA. (C–E) SIRT5 silencing decreased cellular NADPH/NADP+ and GSH/GSSG ratio and increased cellular ROS level. (F) Co-IP of SIRT5 and flag-tagged WT SDHA in HEK293A cells revealed that SIRT5 coimmunoprecipitated with SDHA. (G–J) SIRT5 silencing repressed ACHN and 769-P cell proliferation and colony formation.

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