TNPO3 Antibody - #DF8297
| Product: | TNPO3 Antibody |
| Catalog: | DF8297 |
| Description: | Rabbit polyclonal antibody to TNPO3 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 104 kDa; 104kD(Calculated). |
| Uniprot: | Q9Y5L0 |
| RRID: | AB_2841581 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF8297, RRID:AB_2841581.
Fold/Unfold
TNPO 3; Imp12; Importin 12; Importin-12; IPO12; MTR10A; TNPO3; TNPO3_HUMAN; Transportin 3; Transportin SR; Transportin-3; Transportin-SR; TRN SR; TRN-SR; TRN-SR2; TRNSR;
Immunogens
- Q9Y5L0 TNPO3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEGAKPTLQLVYQAVQALYHDPDPSGKERASFWLGELQRSVHAWEISDQLLQIRQDVESCYFAAQTMKMKIQTSFYELPTDSHASLRDSLLTHIQNLKDLSPVIVTQLALAIADLALQMPSWKGCVQTLVEKYSNDVTSLPFLLEILTVLPEEVHSRSLRIGANRRTEIIEDLAFYSSTVVSLLMTCVEKAGTDEKMLMKVFRCLGSWFNLGVLDSNFMANNKLLALLFEVLQQDKTSSNLHEAASDCVCSALYAIENVETNLPLAMQLFQGVLTLETAYHMAVAREDLDKVLNYCRIFTELCETFLEKIVCTPGQGLGDLRTLELLLICAGHPQYEVVEISFNFWYRLGEHLYKTNDEVIHGIFKAYIQRLLHALARHCQLEPDHEGVPEETDDFGEFRMRVSDLVKDLIFLIGSMECFAQLYSTLKEGNPPWEVTEAVLFIMAAIAKSVDPENNPTLVEVLEGVVRLPETVHTAVRYTSIELVGEMSEVVDRNPQFLDPVLGYLMKGLCEKPLASAAAKAIHNICSVCRDHMAQHFNGLLEIARSLDSFLLSPEAAVGLLKGTALVLARLPLDKITECLSELCSVQVMALKKLLSQEPSNGISSDPTVFLDRLAVIFRHTNPIVENGQTHPCQKVIQEIWPVLSETLNKHRADNRIVERCCRCLRFAVRCVGKGSAALLQPLVTQMVNVYHVHQHSCFLYLGSILVDEYGMEEGCRQGLLDMLQALCIPTFQLLEQQNGLQNHPDTVDDLFRLATRFIQRSPVTLLRSQVVIPILQWAIASTTLDHRDANCSVMRFLRDLIHTGVANDHEEDFELRKELIGQVMNQLGQQLVSQLLHTCCFCLPPYTLPDVAEVLWEIMQVDRPTFCRWLENSLKGLPKETTVGAVTVTHKQLTDFHKQVTSAEECKQVCWALRDFTRLFR
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y5L0 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| K27 | Ubiquitination | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| S74 | Phosphorylation | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| K513 | Ubiquitination | Uniprot | |
| K521 | Ubiquitination | Uniprot | |
| S554 | Phosphorylation | Uniprot | |
| K594 | Ubiquitination | Uniprot | |
| C634 | S-Nitrosylation | Uniprot | |
| K877 | Ubiquitination | Uniprot | |
| K881 | Ubiquitination | Uniprot | |
| T891 | Phosphorylation | Uniprot | |
| K893 | Ubiquitination | Uniprot | |
| T896 | Phosphorylation | Uniprot | |
| K900 | Acetylation | Uniprot | |
| K900 | Ubiquitination | Uniprot | |
| K909 | Ubiquitination | Uniprot |
Research Backgrounds
Importin, which transports target proteins into the nucleus. Specifically mediates the nuclear import of splicing factor serine/arginine (SR) proteins, such as RBM4, SFRS1 and SFRS2, by recognizing phosphorylated SR domains. Also mediates the nuclear import of serine/arginine (SR) protein CPSF6, independently of CPSF6 phosphorylation. The nuclear import process is regulated by the small GTPase Ran that partitions between cytoplasm and nucleus in the predominantly GDP- and GTP-bound form, respectively. Importin associates with target cargo proteins in the cytoplasm, and the competitive binding of GTP-bound Ran induces the release of cargos in the nucleus.
(Microbial infection) Involved in immunodeficiency virus (HIV-1) infection by importing the pre-integration complex (PIC) into the nucleus. Required for a nuclear maturation step of HIV-1 prior to integration.
Nucleus envelope. Cytoplasm.
Note: Localizes to the nuclear envelope and annulate lamellae, which consists in stacks of endoplasmic reticulum membranes containing a high density of nuclear pores.
Expressed in skeletal muscle.
Interacts with (GTP-bound) Ran. Interacts with (phosphorylated) SFRS1 and SFRS2; leading to their nuclear import. Interacts with NUP62. Interacts with RBM4. Interacts with CPSF6, promoting its nuclear import.
(Microbial infection) Interacts with the HIV-1 pre-integration complex (PIC), which is composed of viral genome, matrix protein, Vpr and integrase. Interacts with HIV-1 integrase protein; the interaction is direct.
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