TRPM7 Antibody | Affinity Biosciences

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TRPM7 Antibody - Western blot analysis of TRPM7 using HeLa whole cell lysates.

Product:	TRPM7 Antibody
Catalog:	DF8300
Description:	Rabbit polyclonal antibody to TRPM7
Application:	WB
Reactivity:	Human
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	213 kDa; 213kD(Calculated).
Uniprot:	Q96QT4
RRID:	AB_2841583

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Pig(100%), Bovine(91%), Horse(82%), Sheep(91%), Rabbit(100%), Dog(91%)

Clonality:

Polyclonal

Specificity:

TRPM7 Antibody detects endogenous levels of total TRPM7.

RRID:

AB_2841583
Cite Format: Affinity Biosciences Cat# DF8300, RRID:AB_2841583.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ALSPDC; CHAK; CHAK1; Channel kinase 1; Channel-kinase 1; Long transient receptor potential channel 7; LTrpC-7; LTrpC7; Transient receptor potential cation channel subfamily M member 7; TRP PLIK; TRPM7; TRPM7_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q96QT4(TRPM7_HUMAN) >>Visit HPA database.

Gene(ID):

TRPM7(54822)

Sequence:

Q96QT4 TRPM7_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSQKSWIESTLTKRECVYIIPSSKDPHRCLPGCQICQQLVRCFCGRLVKQHACFTASLAMKYSDVKLGDHFNQAIEEWSVEKHTEQSPTDAYGVINFQGGSHSYRAKYVRLSYDTKPEVILQLLLKEWQMELPKLVISVHGGMQKFELHPRIKQLLGKGLIKAAVTTGAWILTGGVNTGVAKHVGDALKEHASRSSRKICTIGIAPWGVIENRNDLVGRDVVAPYQTLLNPLSKLNVLNNLHSHFILVDDGTVGKYGAEVRLRRELEKTINQQRIHARIGQGVPVVALIFEGGPNVILTVLEYLQESPPVPVVVCEGTGRAADLLAYIHKQTEEGGNLPDAAEPDIISTIKKTFNFGQNEALHLFQTLMECMKRKELITVFHIGSDEHQDIDVAILTALLKGTNASAFDQLILTLAWDRVDIAKNHVFVYGQQWLVGSLEQAMLDALVMDRVAFVKLLIENGVSMHKFLTIPRLEELYNTKQGPTNPMLFHLVRDVKQGNLPPGYKITLIDIGLVIEYLMGGTYRCTYTRKRFRLIYNSLGGNNRRSGRNTSSSTPQLRKSHESFGNRADKKEKMRHNHFIKTAQPYRPKIDTVMEEGKKKRTKDEIVDIDDPETKRFPYPLNELLIWACLMKRQVMARFLWQHGEESMAKALVACKIYRSMAYEAKQSDLVDDTSEELKQYSNDFGQLAVELLEQSFRQDETMAMKLLTYELKNWSNSTCLKLAVSSRLRPFVAHTCTQMLLSDMWMGRLNMRKNSWYKVILSILVPPAILLLEYKTKAEMSHIPQSQDAHQMTMDDSENNFQNITEEIPMEVFKEVRILDSNEGKNEMEIQMKSKKLPITRKFYAFYHAPIVKFWFNTLAYLGFLMLYTFVVLVQMEQLPSVQEWIVIAYIFTYAIEKVREIFMSEAGKVNQKIKVWFSDYFNISDTIAIISFFIGFGLRFGAKWNFANAYDNHVFVAGRLIYCLNIIFWYVRLLDFLAVNQQAGPYVMMIGKMVANMFYIVVIMALVLLSFGVPRKAILYPHEAPSWTLAKDIVFHPYWMIFGEVYAYEIDVCANDSVIPQICGPGTWLTPFLQAVYLFVQYIIMVNLLIAFFNNVYLQVKAISNIVWKYQRYHFIMAYHEKPVLPPPLIILSHIVSLFCCICKRRKKDKTSDGPKLFLTEEDQKKLHDFEEQCVEMYFNEKDDKFHSGSEERIRVTFERVEQMCIQIKEVGDRVNYIKRSLQSLDSQIGHLQDLSALTVDTLKTLTAQKASEASKVHNEITRELSISKHLAQNLIDDGPVRPSVWKKHGVVNTLSSSLPQGDLESNNPFHCNILMKDDKDPQCNIFGQDLPAVPQRKEFNFPEAGSSSGALFPSAVSPPELRQRLHGVELLKIFNKNQKLGSSSTSIPHLSSPPTKFFVSTPSQPSCKSHLETGTKDQETVCSKATEGDNTEFGAFVGHRDSMDLQRFKETSNKIKILSNNNTSENTLKRVSSLAGFTDCHRTSIPVHSKQAEKISRRPSTEDTHEVDSKAALIPDWLQDRPSNREMPSEEGTLNGLTSPFKPAMDTNYYYSAVERNNLMRLSQSIPFTPVPPRGEPVTVYRLEESSPNILNNSMSSWSQLGLCAKIEFLSKEEMGGGLRRAVKVQCTWSEHDILKSGHLYIIKSFLPEVVNTWSSIYKEDTVLHLCLREIQQQRAAQKLTFAFNQMKPKSIPYSPRFLEVFLLYCHSAGQWFAVEECMTGEFRKYNNNNGDEIIPTNTLEEIMLAFSHWTYEYTRGELLVLDLQGVGENLTDPSVIKAEEKRSCDMVFGPANLGEDAIKNFRAKHHCNSCCRKLKLPDLKRNDYTPDKIIFPQDEPSDLNLQPGNSTKESESTNSVRLML

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Pig

100

Bovine

Sheep

Dog

Horse

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96QT4 As Substrate

Q96QT4

Site	PTM Type	Enzyme	Source
M1	Acetylation		Uniprot
S5	Phosphorylation		Uniprot
T12	Phosphorylation		Uniprot
S57	Phosphorylation		Uniprot
K66	Ubiquitination		Uniprot
S101	Phosphorylation		Uniprot
K189	Ubiquitination		Uniprot
S233	Phosphorylation		Uniprot
K351	Ubiquitination		Uniprot
T523	Phosphorylation		Uniprot
S554	Phosphorylation		Uniprot
T555	Phosphorylation		Uniprot
K667	Ubiquitination		Uniprot
S764	Phosphorylation		Uniprot
Y776	Phosphorylation		Uniprot
T778	Phosphorylation		Uniprot
K827	Ubiquitination		Uniprot
S907	Phosphorylation		Uniprot
K1159	Ubiquitination		Uniprot
T1163	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
K1168	Acetylation		Uniprot
S1191	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
S1193	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
S1230	Phosphorylation		Uniprot
S1239	Phosphorylation		Uniprot
K1253	Ubiquitination		Uniprot
S1255	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
S1258	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
T1265	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
S1269	Phosphorylation		Uniprot
S1271	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
S1287	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
S1358	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
S1361	Phosphorylation		Uniprot
S1386	Phosphorylation		Uniprot
S1387	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
S1390	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
S1395	Phosphorylation		Uniprot
S1396	Phosphorylation		Uniprot
S1404	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
T1405	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
S1407	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
S1410	Phosphorylation		Uniprot
S1413	Phosphorylation		Uniprot
T1417	Phosphorylation		Uniprot
T1419	Phosphorylation		Uniprot
T1435	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
S1446	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
T1455	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
S1456	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
S1463	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
S1468	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
T1471	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
K1473	Methylation		Uniprot
K1473	Ubiquitination		Uniprot
S1476	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
S1477	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
T1482	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
S1488	Phosphorylation		Uniprot
S1493	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
S1500	Phosphorylation		Uniprot
S1504	Phosphorylation		Uniprot
T1505	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
T1508	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
S1513	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
S1527	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
S1533	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
T1537	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
T1542	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
S1543	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
T1551	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
S1567	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
S1569	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
T1583	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
S1598	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
S1615	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
S1660	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
T1685	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
S1695	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
S1779	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
T1830	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
K1833	Ubiquitination		Uniprot
S1851	Phosphorylation	Q96QT4 (TRPM7)	Uniprot
S1860	Phosphorylation	Q96QT4 (TRPM7)	Uniprot

PTMs - Q96QT4 As Enzyme

Q96QT4

Substrate	Site	Source
O00418 (EEF2K)	S78	Uniprot
P04083 (ANXA1)	S5	Uniprot
P28289 (TMOD1)	S2	Uniprot
P28289 (TMOD1)	T54	Uniprot
P35579 (MYH9)	T1800	Uniprot
P35579 (MYH9)	S1803	Uniprot
P35579 (MYH9)	S1808	Uniprot
Q96QT4 (TRPM7)	T1163	Uniprot
Q96QT4 (TRPM7)	S1191	Uniprot
Q96QT4 (TRPM7)	S1193	Uniprot
Q96QT4 (TRPM7)	S1255	Uniprot
Q96QT4 (TRPM7)	S1258	Uniprot
Q96QT4 (TRPM7)	T1265	Uniprot
Q96QT4 (TRPM7)	S1271	Uniprot
Q96QT4 (TRPM7)	S1287	Uniprot
Q96QT4 (TRPM7)	S1358	Uniprot
Q96QT4 (TRPM7)	S1387	Uniprot
Q96QT4 (TRPM7)	S1390	Uniprot
Q96QT4 (TRPM7)	S1404	Uniprot
Q96QT4 (TRPM7)	T1405	Uniprot
Q96QT4 (TRPM7)	S1407	Uniprot
Q96QT4 (TRPM7)	T1435	Uniprot
Q96QT4 (TRPM7)	S1446	Uniprot
Q96QT4 (TRPM7)	T1455	Uniprot
Q96QT4 (TRPM7)	S1456	Uniprot
Q96QT4 (TRPM7)	S1463	Uniprot
Q96QT4 (TRPM7)	S1468	Uniprot
Q96QT4 (TRPM7)	T1471	Uniprot
Q96QT4 (TRPM7)	S1476	Uniprot
Q96QT4 (TRPM7)	S1477	Uniprot
Q96QT4 (TRPM7)	T1482	Uniprot
Q96QT4 (TRPM7)	S1493	Uniprot
Q96QT4 (TRPM7)	T1505	Uniprot
Q96QT4 (TRPM7)	T1508	Uniprot
Q96QT4 (TRPM7)	S1513	Uniprot
Q96QT4 (TRPM7)	S1527	Uniprot
Q96QT4 (TRPM7)	S1533	Uniprot
Q96QT4 (TRPM7)	T1537	Uniprot
Q96QT4 (TRPM7)	T1542	Uniprot
Q96QT4 (TRPM7)	S1543	Uniprot
Q96QT4 (TRPM7)	T1551	Uniprot
Q96QT4 (TRPM7)	S1567	Uniprot
Q96QT4 (TRPM7)	S1569	Uniprot
Q96QT4 (TRPM7)	T1583	Uniprot
Q96QT4 (TRPM7)	S1598	Uniprot
Q96QT4 (TRPM7)	S1615	Uniprot
Q96QT4 (TRPM7)	S1660	Uniprot
Q96QT4 (TRPM7)	T1685	Uniprot
Q96QT4 (TRPM7)	S1695	Uniprot
Q96QT4 (TRPM7)	S1779	Uniprot
Q96QT4 (TRPM7)	T1830	Uniprot
Q96QT4 (TRPM7)	S1851	Uniprot
Q96QT4 (TRPM7)	S1860	Uniprot

Research Backgrounds

Q96QT4_TRPM7_HUMAN

Function:

Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. Involved in TNF-induced necroptosis downstream of MLKL by mediating calcium influx. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).

PTMs:

Autophosphorylated.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Subunit Structure:

Homodimer. Interacts with PLCB1 (By similarity). Forms heterodimers with TRPM6.

Family&Domains:

In the C-terminal section; belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.

In the N-terminal section; belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM7 sub-subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)

· Organismal Systems > Digestive system > Mineral absorption.

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TRPM7 Antibody - #DF8300