BAZ1B Antibody - #DF8304

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Product: BAZ1B Antibody
Catalog: DF8304
Description: Rabbit polyclonal antibody to BAZ1B
Application: WB
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 185 kDa; 171kD(Calculated).
Uniprot: Q9UIG0
RRID: AB_2841586

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(92%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(92%)
Clonality:
Polyclonal
Specificity:
BAZ1B Antibody detects endogenous levels of total BAZ1B.
RRID:
AB_2841586
Cite Format: Affinity Biosciences Cat# DF8304, RRID:AB_2841586.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

baz1b; BAZ1B_HUMAN; Bromodomain adjacent to zinc finger domain protein 1B; hWALP 2; hWALP-2; hWALP2; transcription factor WSTF; Tyrosine-protein kinase BAZ1B; WALP-2; WALP2; WBRS 9; WBRS-9; WBRS9; WBSC 10; WBSC-10; WBSC10; WBSCR10; WBSCR9; Williams Beuren syndrome chromosome region 9 protein; Williams syndrome transcription factor; Williams-Beuren syndrome chromosomal region 10 protein; Williams-Beuren syndrome chromosomal region 9 protein; WSTF;

Immunogens

Immunogen:
Uniprot:

Q9UIG0(BAZ1B_HUMAN) >>Visit HPA database.

Gene(ID):
BAZ1B(9031)
Expression:
Q9UIG0 BAZ1B_HUMAN:

Ubiquitously expressed with high levels of expression in heart, brain, placenta, skeletal muscle and ovary.

Sequence:
MAPLLGRKPFPLVKPLPGEEPLFTIPHTQEAFRTREEYEARLERYSERIWTCKSTGSSQLTHKEAWEEEQEVAELLKEEFPAWYEKLVLEMVHHNTASLEKLVDTAWLEIMTKYAVGEECDFEVGKEKMLKVKIVKIHPLEKVDEEATEKKSDGACDSPSSDKENSSQIAQDHQKKETVVKEDEGRRESINDRARRSPRKLPTSLKKGERKWAPPKFLPHKYDVKLQNEDKIISNVPADSLIRTERPPNKEIVRYFIRHNALRAGTGENAPWVVEDELVKKYSLPSKFSDFLLDPYKYMTLNPSTKRKNTGSPDRKPSKKSKTDNSSLSSPLNPKLWCHVHLKKSLSGSPLKVKNSKNSKSPEEHLEEMMKMMSPNKLHTNFHIPKKGPPAKKPGKHSDKPLKAKGRSKGILNGQKSTGNSKSPKKGLKTPKTKMKQMTLLDMAKGTQKMTRAPRNSGGTPRTSSKPHKHLPPAALHLIAYYKENKDREDKRSALSCVISKTARLLSSEDRARLPEELRSLVQKRYELLEHKKRWASMSEEQRKEYLKKKREELKKKLKEKAKERREKEMLERLEKQKRYEDQELTGKNLPAFRLVDTPEGLPNTLFGDVAMVVEFLSCYSGLLLPDAQYPITAVSLMEALSADKGGFLYLNRVLVILLQTLLQDEIAEDYGELGMKLSEIPLTLHSVSELVRLCLRRSDVQEESEGSDTDDNKDSAAFEDNEVQDEFLEKLETSEFFELTSEEKLQILTALCHRILMTYSVQDHMETRQQMSAELWKERLAVLKEENDKKRAEKQKRKEMEAKNKENGKVENGLGKTDRKKEIVKFEPQVDTEAEDMISAVKSRRLLAIQAKKEREIQEREMKVKLERQAEEERIRKHKAAAEKAFQEGIAKAKLVMRRTPIGTDRNHNRYWLFSDEVPGLFIEKGWVHDSIDYRFNHHCKDHTVSGDEDYCPRSKKANLGKNASMNTQHGTATEVAVETTTPKQGQNLWFLCDSQKELDELLNCLHPQGIRESQLKERLEKRYQDIIHSIHLARKPNLGLKSCDGNQELLNFLRSDLIEVATRLQKGGLGYVEETSEFEARVISLEKLKDFGECVIALQASVIKKFLQGFMAPKQKRRKLQSEDSAKTEEVDEEKKMVEEAKVASALEKWKTAIREAQTFSRMHVLLGMLDACIKWDMSAENARCKVCRKKGEDDKLILCDECNKAFHLFCLRPALYEVPDGEWQCPACQPATARRNSRGRNYTEESASEDSEDDESDEEEEEEEEEEEEEDYEVAGLRLRPRKTIRGKHSVIPPAARSGRRPGKKPHSTRRSQPKAPPVDDAEVDELVLQTKRSSRRQSLELQKCEEILHKIVKYRFSWPFREPVTRDEAEDYYDVITHPMDFQTVQNKCSCGSYRSVQEFLTDMKQVFTNAEVYNCRGSHVLSCMVKTEQCLVALLHKHLPGHPYVRRKRKKFPDRLAEDEGDSEPEAVGQSRGRRQKK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Rabbit
100
Bovine
92
Dog
92
Chicken
73
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UIG0 As Substrate

Site PTM Type Enzyme
K14 Ubiquitination
S46 Phosphorylation
K53 Ubiquitination
S58 Phosphorylation
K63 Ubiquitination
K77 Ubiquitination
K136 Ubiquitination
K142 Ubiquitination
K151 Acetylation
S152 Phosphorylation
S158 Phosphorylation P27361 (MAPK3) , P45984 (MAPK9) , Q16539 (MAPK14)
S160 Phosphorylation
S161 Phosphorylation
S166 Phosphorylation
S167 Phosphorylation
K175 Acetylation
K175 Methylation
K181 Methylation
S189 Phosphorylation
S197 Phosphorylation
T203 Phosphorylation
S204 Phosphorylation
K216 Acetylation
K221 Acetylation
K221 Ubiquitination
Y222 Phosphorylation
K225 Sumoylation
K231 Ubiquitination
S234 Phosphorylation
K250 Ubiquitination
T266 Phosphorylation
K280 Ubiquitination
Y282 Phosphorylation
K287 Ubiquitination
S289 Phosphorylation
Y296 Phosphorylation
K297 Acetylation
K297 Ubiquitination
Y298 Phosphorylation
K306 Acetylation
K306 Ubiquitination
S312 Phosphorylation
S318 Phosphorylation
S321 Phosphorylation
K322 Ubiquitination
T323 Phosphorylation
S326 Phosphorylation
S327 Phosphorylation
S330 Phosphorylation
K335 Ubiquitination
K343 Ubiquitination
K344 Methylation
K344 Ubiquitination
S345 Phosphorylation
S347 Phosphorylation
S349 Phosphorylation
K352 Ubiquitination
S359 Phosphorylation
S361 Phosphorylation
S374 Phosphorylation
K386 Ubiquitination
K409 Acetylation
K416 Acetylation
K426 Acetylation
K429 Acetylation
T430 Phosphorylation
K432 Acetylation
K445 Ubiquitination
K449 Acetylation
T451 Phosphorylation
S457 Phosphorylation
S465 Phosphorylation
K483 Ubiquitination
K501 Ubiquitination
S508 Phosphorylation
K532 Ubiquitination
K588 Ubiquitination
S699 Phosphorylation
S705 Phosphorylation
S708 Phosphorylation
T710 Phosphorylation
S716 Phosphorylation
T759 Phosphorylation
Y760 Phosphorylation
K778 Ubiquitination
K810 Acetylation
K817 Acetylation
K826 Sumoylation
T833 Phosphorylation
S840 Phosphorylation
K843 Ubiquitination
S844 Phosphorylation
K853 Sumoylation
K853 Ubiquitination
K864 Acetylation
K885 Ubiquitination
K893 Acetylation
K893 Ubiquitination
S932 Phosphorylation
Y935 Phosphorylation
K942 Ubiquitination
T945 Phosphorylation
S947 Phosphorylation
Y952 Phosphorylation
S966 Phosphorylation
T969 Phosphorylation
T973 Phosphorylation
T983 Phosphorylation
K1043 Ubiquitination
K1068 Ubiquitination
S1078 Phosphorylation
K1089 Sumoylation
K1089 Ubiquitination
K1107 Sumoylation
K1116 Acetylation
K1118 Acetylation
S1127 Phosphorylation
K1129 Sumoylation
S1147 Phosphorylation
K1291 Ubiquitination
S1315 Phosphorylation
K1318 Ubiquitination
K1335 Acetylation
K1335 Ubiquitination
S1338 Phosphorylation
S1342 Phosphorylation
K1347 Ubiquitination
K1354 Ubiquitination
S1468 Phosphorylation
S1476 Phosphorylation

PTMs - Q9UIG0 As Enzyme

Substrate Site Source
P16104 (H2AFX) Y143 Uniprot

Research Backgrounds

Function:

Atypical tyrosine-protein kinase that plays a central role in chromatin remodeling and acts as a transcription regulator. Involved in DNA damage response by phosphorylating 'Tyr-142' of histone H2AX (H2AXY142ph). H2AXY142ph plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes, has a role in RNA polymerase I and RNA polymerase III transcription, mediates the histone H2AX phosphorylation at 'Tyr-142', and is involved in the maintenance of chromatin structures during DNA replication processes. In the complex, it mediates the recruitment of the WICH complex to replication foci during DNA replication.

Subcellular Location:

Nucleus.
Note: Accumulates in pericentromeric heterochromatin during replication. Targeted to replication foci throughout S phase via its association with PCNA.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed with high levels of expression in heart, brain, placenta, skeletal muscle and ovary.

Subunit Structure:

Interacts with MYO1C (By similarity). Interacts with CDT1. Interacts with SMARCA5/SNF2H; the interaction is direct and forms the WICH complex. Component of the B-WICH complex, at least composed of SMARCA5/SNF2H, BAZ1B/WSTF, SF3B1, DEK, MYO1C, ERCC6, MYBBP1A and DDX21. Interacts with PCNA; the interaction is direct.

Family&Domains:

Belongs to the WAL family. BAZ1B subfamily.

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