PKP3 Antibody - #DF8310

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Product: PKP3 Antibody
Catalog: DF8310
Description: Rabbit polyclonal antibody to PKP3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Sheep, Dog, Chicken
Mol.Wt.: 87 kDa; 87kD(Calculated).
Uniprot: Q9Y446
RRID: AB_2841591

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Dog(100%), Chicken(82%)
Clonality:
Polyclonal
Specificity:
PKP3 Antibody detects endogenous levels of total PKP3.
RRID:
AB_2841591
Cite Format: Affinity Biosciences Cat# DF8310, RRID:AB_2841591.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

PKP 3; PKP3; PKP3_HUMAN; Plakophilin 3b; Plakophilin-3; Plakophilin3;

Immunogens

Immunogen:
Uniprot:

Q9Y446(PKP3_HUMAN) >>Visit HPA database.

Gene(ID):
PKP3(11187)
Expression:
Q9Y446 PKP3_HUMAN:

Isoform PKP3a is found in desmosomes of most simple and stratified epithelia. Not found in foreskin fibroblasts and various sarcoma-derived cell lines. Beside dendritic reticular cells of lymphatic follicles not found in non-epithelial desmosome-bearing tissues. Isoform PKP3b is abundant in the desmosomes of stratified epithelial cell but absent in simple epithelial cells, it is also expressed in the colon and its tumors.

Sequence:
MQDGNFLLSALQPEAGVCSLALPSDLQLDRRGAEGPEAERLRAARVQEQVRARLLQLGQQPRHNGAAEPEPEAETARGTSRGQYHTLQAGFSSRSQGLSGDKTSGFRPIAKPAYSPASWSSRSAVDLSCSRRLSSAHNGGSAFGAAGYGGAQPTPPMPTRPVSFHERGGVGSRADYDTLSLRSLRLGPGGLDDRYSLVSEQLEPAATSTYRAFAYERQASSSSSRAGGLDWPEATEVSPSRTIRAPAVRTLQRFQSSHRSRGVGGAVPGAVLEPVARAPSVRSLSLSLADSGHLPDVHGFNSYGSHRTLQRLSSGFDDIDLPSAVKYLMASDPNLQVLGAAYIQHKCYSDAAAKKQARSLQAVPRLVKLFNHANQEVQRHATGAMRNLIYDNADNKLALVEENGIFELLRTLREQDDELRKNVTGILWNLSSSDHLKDRLARDTLEQLTDLVLSPLSGAGGPPLIQQNASEAEIFYNATGFLRNLSSASQATRQKMRECHGLVDALVTSINHALDAGKCEDKSVENAVCVLRNLSYRLYDEMPPSALQRLEGRGRRDLAGAPPGEVVGCFTPQSRRLRELPLAADALTFAEVSKDPKGLEWLWSPQIVGLYNRLLQRCELNRHTTEAAAGALQNITAGDRRWAGVLSRLALEQERILNPLLDRVRTADHHQLRSLTGLIRNLSRNARNKDEMSTKVVSHLIEKLPGSVGEKSPPAEVLVNIIAVLNNLVVASPIAARDLLYFDGLRKLIFIKKKRDSPDSEKSSRAASSLLANLWQYNKLHRDFRAKGYRKEDFLGP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
82
Xenopus
58
Horse
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y446 As Substrate

Site PTM Type Enzyme
R81 Methylation
Y84 Phosphorylation
T86 Phosphorylation
S92 Phosphorylation
S95 Phosphorylation
K102 Ubiquitination
Y114 Phosphorylation
S115 Phosphorylation
S118 Phosphorylation
S123 Phosphorylation
S130 Phosphorylation
S134 Phosphorylation
Y148 Phosphorylation
T154 Phosphorylation
S163 Phosphorylation
S172 Phosphorylation
Y176 Phosphorylation
T178 Phosphorylation
S180 Phosphorylation
S183 Phosphorylation
Y195 Phosphorylation
S196 Phosphorylation
S199 Phosphorylation
T207 Phosphorylation
S208 Phosphorylation
T209 Phosphorylation
Y210 Phosphorylation
Y215 Phosphorylation
S220 Phosphorylation
S238 Phosphorylation
S240 Phosphorylation
T250 Phosphorylation
S260 Phosphorylation
R261 Methylation
S280 Phosphorylation
S283 Phosphorylation
S285 Phosphorylation
S287 Phosphorylation
S291 Phosphorylation
S302 Phosphorylation
Y303 Phosphorylation
S305 Phosphorylation
T308 Phosphorylation
S313 Phosphorylation
S314 Phosphorylation
Y327 Phosphorylation
S331 Phosphorylation
Y342 Phosphorylation
S349 Phosphorylation
S359 Phosphorylation
Y390 Phosphorylation
K396 Ubiquitination
K518 Ubiquitination
Y539 Phosphorylation
T571 Phosphorylation
S574 Phosphorylation
K594 Ubiquitination
T666 Phosphorylation
K695 Ubiquitination
K703 Ubiquitination
K752 Acetylation
K791 Ubiquitination

Research Backgrounds

Function:

May play a role in junctional plaques.

Subcellular Location:

Nucleus. Cell junction>Desmosome.
Note: Nuclear and associated with desmosomes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform PKP3a is found in desmosomes of most simple and stratified epithelia. Not found in foreskin fibroblasts and various sarcoma-derived cell lines. Beside dendritic reticular cells of lymphatic follicles not found in non-epithelial desmosome-bearing tissues. Isoform PKP3b is abundant in the desmosomes of stratified epithelial cell but absent in simple epithelial cells, it is also expressed in the colon and its tumors.

Family&Domains:

Belongs to the beta-catenin family.

References

1). MCP-enhanced SOD3 activity inhibits gastric cancer and potentiate chemotherapy via modulating EGFR signaling. Life sciences, 2024 (PubMed: 39746602) [IF=6.1]

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