PANX1 Antibody - #DF8316

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Product: PANX1 Antibody
Catalog: DF8316
Description: Rabbit polyclonal antibody to PANX1
Application: WB IF/ICC
Cited expt.: WB, IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 48 kDa; 48kD(Calculated).
Uniprot: Q96RD7
RRID: AB_2841594

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
PANX1 Antibody detects endogenous levels of total PANX1.
RRID:
AB_2841594
Cite Format: Affinity Biosciences Cat# DF8316, RRID:AB_2841594.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

innexin; MRS1; Pannexin 1; Pannexin-1; Panx1; PANX1_HUMAN; PX1; UNQ2529;

Immunogens

Immunogen:

A synthesized peptide derived from human PANX1, corresponding to a region within the internal amino acids.

Uniprot:

Q96RD7(PANX1_HUMAN) >>Visit HPA database.

Gene(ID):
PANX1(24145)
Sequence:
MAIAQLATEYVFSDFLLKEPTEPKFKGLRLELAVDKMVTCIAVGLPLLLISLAFAQEISIGTQISCFSPSSFSWRQAAFVDSYCWAAVQQKNSLQSESGNLPLWLHKFFPYILLLFAILLYLPPLFWRFAAAPHICSDLKFIMEELDKVYNRAIKAAKSARDLDMRDGACSVPGVTENLGQSLWEVSESHFKYPIVEQYLKTKKNSNNLIIKYISCRLLTLIIILLACIYLGYYFSLSSLSDEFVCSIKSGILRNDSTVPDQFQCKLIAVGIFQLLSVINLVVYVLLAPVVVYTLFVPFRQKTDVLKVYEILPTFDVLHFKSEGYNDLSLYNLFLEENISEVKSYKCLKVLENIKSSGQGIDPMLLLTNLGMIKMDVVDGKTPMSAEMREEQGNQTAELQGMNIDSETKANNGEKNARQRLLDSSC

Research Backgrounds

Function:

Structural component of the gap junctions and the hemichannels. May play a role as a Ca(2+)-leak channel to regulate ER Ca(2+) homeostasis.

PTMs:

S-nitrosylation inhibits channel currents and ATP release.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Cell junction>Gap junction. Endoplasmic reticulum membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the pannexin family.

Research Fields

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

References

1). Processing of angiocrine alarmin IL-1α in endothelial cells promotes lung and liver fibrosis. International immunopharmacology, 2024 (PubMed: 38723369) [IF=4.8]
2). Carbenoxolone has the potential to ameliorate acute incision pain in rats. Molecular Medicine Reports, 2021 (PubMed: 34013377) [IF=3.4]

Application: WB    Species: Rat    Sample:

Figure 2. Px1 and Cx43 expression in the spinal cords of IP model rats. Expression of Px1 and Cx42 in rats was detected via western blotting at (A) 2, (B) 4, (C) 6 and (D) 24 h post-surgery. The scanning density of (E) Px1 and (F) Cx43 expression normalized to control. *P<0.05, **P<0.01 and ***P<0.001 vs. C group; #P<0.05, ##P<0.01 and ###P<0.001 vs. NS group; ΔP<0.05, ΔΔP<0.01 and ΔΔΔP<0.001 vs. CBX group. Data are presented as the mean ± SD. C, control group; IP, incision pain; NS, normal saline; CBX, carbenoxolone; 10panx, pannexin-1 mimetic inhibitory peptide; Px1, pannexin 1; Cx43, connexin 43.

Application: IF/ICC    Species: Rat    Sample:

Figure 3. Co-localization of Px1 and GFAP in IP model rats. Px1 (green) and GFAP (red) expression was analyzed using an immunofluorescence assay. Magnification, ×200; scale bar, 10 µm. C, control group; IP, incision pain; CBX, carbenoxolone; 10panx, pannexin-1 mimetic inhibitory peptide; Px1, pannexin 1; GFAP, glial fibrillary acidic protein.

Application: IF/ICC    Species: rat    Sample:

Figure 3. |Co‑localization of Px1 and GFAP in IP model rats. Px1 (green) and GFAP (red)expression was analyzed using an immunofluorescence assay.Magnification, x200; scale bar, 10 µm. C,control group; IP, incision pain; CBX, carbenoxolone; 10panx, pannexin‑1 mimetic inhibitory peptide; Px1, pannexin 1;GFAP, glial fibrillary acidic protein

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