HYOU1 Antibody | Affinity Biosciences

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IF/ICC

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HYOU1 Antibody - Western blot analysis of HYOU1 using COS7 whole cell lysates.

HYOU1 Antibody - DF8329 staining Hela cells by IF/ICC.

Product:	HYOU1 Antibody
Catalog:	DF8329
Description:	Rabbit polyclonal antibody to HYOU1
Application:	WB IF/ICC
Reactivity:	Human, Monkey
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	111 kDa; 111kD(Calculated).
Uniprot:	Q9Y4L1
RRID:	AB_2841601

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Monkey

Prediction:

Pig(100%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

HYOU1 Antibody detects endogenous levels of total HYOU1.

RRID:

AB_2841601
Cite Format: Affinity Biosciences Cat# DF8329, RRID:AB_2841601.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

150 kDa oxygen regulated protein; 150 kDa oxygen-regulated protein; 170 kDa glucose regulated protein; 170 kDa glucose-regulated protein; DKFZp686N08236; FLJ94899; FLJ97572; glucose regulated protein 170; Grp 170; GRP-170; Grp170; HSP 12A; HSP12A; Hyou1; HYOU1_HUMAN; Hypoxia up regulated 1; hypoxia up-regulated 1 precursor; Hypoxia up-regulated protein 1; Hypoxia upregulated 1; Orp 150; ORP-150; Orp150; oxygen regulated protein (150kD);

Immunogens

Immunogen:

Uniprot:

Q9Y4L1(HYOU1_HUMAN) >>Visit HPA database.

Gene(ID):

HYOU1(10525)

Expression:

Q9Y4L1 HYOU1_HUMAN:

Highly expressed in tissues that contain well-developed endoplasmic reticulum and synthesize large amounts of secretory proteins. Highly expressed in liver and pancreas and lower expression in brain and kidney. Also expressed in macrophages within aortic atherosclerotic plaques, and in breast cancers.

Sequence:

Q9Y4L1 HYOU1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MADKVRRQRPRRRVCWALVAVLLADLLALSDTLAVMSVDLGSESMKVAIVKPGVPMEIVLNKESRRKTPVIVTLKENERFFGDSAASMAIKNPKATLRYFQHLLGKQADNPHVALYQARFPEHELTFDPQRQTVHFQISSQLQFSPEEVLGMVLNYSRSLAEDFAEQPIKDAVITVPVFFNQAERRAVLQAARMAGLKVLQLINDNTATALSYGVFRRKDINTTAQNIMFYDMGSGSTVCTIVTYQMVKTKEAGMQPQLQIRGVGFDRTLGGLEMELRLRERLAGLFNEQRKGQRAKDVRENPRAMAKLLREANRLKTVLSANADHMAQIEGLMDDVDFKAKVTRVEFEELCADLFERVPGPVQQALQSAEMSLDEIEQVILVGGATRVPRVQEVLLKAVGKEELGKNINADEAAAMGAVYQAAALSKAFKVKPFVVRDAVVYPILVEFTREVEEEPGIHSLKHNKRVLFSRMGPYPQRKVITFNRYSHDFNFHINYGDLGFLGPEDLRVFGSQNLTTVKLKGVGDSFKKYPDYESKGIKAHFNLDESGVLSLDRVESVFETLVEDSAEEESTLTKLGNTISSLFGGGTTPDAKENGTDTVQEEEESPAEGSKDEPGEQVELKEEAEAPVEDGSQPPPPEPKGDATPEGEKATEKENGDKSEAQKPSEKAEAGPEGVAPAPEGEKKQKPARKRRMVEEIGVELVVLDLPDLPEDKLAQSVQKLQDLTLRDLEKQEREKAANSLEAFIFETQDKLYQPEYQEVSTEEQREEISGKLSAASTWLEDEGVGATTVMLKEKLAELRKLCQGLFFRVEERKKWPERLSALDNLLNHSSMFLKGARLIPEMDQIFTEVEMTTLEKVINETWAWKNATLAEQAKLPATEKPVLLSKDIEAKMMALDREVQYLLNKAKFTKPRPRPKDKNGTRAEPPLNASASDQGEKVIPPAGQTEDAEPISEPEKVETGSEPGDTEPLELGGPGAEPEQKEQSTGQKRPLKNDEL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Dog

100

Rabbit

100

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y4L1 As Substrate

Q9Y4L1

Site	PTM Type	Source
K51	Ubiquitination	Uniprot
K62	Ubiquitination	Uniprot
K75	Ubiquitination	Uniprot
R79	Methylation	Uniprot
K91	Ubiquitination	Uniprot
K94	Ubiquitination	Uniprot
K106	Ubiquitination	Uniprot
Y116	Phosphorylation	Uniprot
N155	N-Glycosylation	Uniprot
K198	Acetylation	Uniprot
K198	Ubiquitination	Uniprot
Y213	Phosphorylation	Uniprot
R262	Methylation	Uniprot
T269	Phosphorylation	Uniprot
R278	Methylation	Uniprot
K308	Ubiquitination	Uniprot
K398	Ubiquitination	Uniprot
K402	Ubiquitination	Uniprot
Y421	Phosphorylation	Uniprot
K433	Ubiquitination	Uniprot
Y443	Phosphorylation	Uniprot
K463	Acetylation	Uniprot
K463	Ubiquitination	Uniprot
N515	N-Glycosylation	Uniprot
K522	Ubiquitination	Uniprot
S527	Phosphorylation	Uniprot
K529	Ubiquitination	Uniprot
K537	Ubiquitination	Uniprot
S567	Phosphorylation	Uniprot
T580	O-Glycosylation	Uniprot
T589	O-Glycosylation	Uniprot
T590	O-Glycosylation	Uniprot
T590	Phosphorylation	Uniprot
N596	N-Glycosylation	Uniprot
T600	Phosphorylation	Uniprot
S634	Phosphorylation	Uniprot
T727	Phosphorylation	Uniprot
K738	Ubiquitination	Uniprot
S742	Phosphorylation	Uniprot
K753	Ubiquitination	Uniprot
Y759	Phosphorylation	Uniprot
S763	Phosphorylation	Uniprot
N830	N-Glycosylation	Uniprot
T850	Phosphorylation	Uniprot
N862	N-Glycosylation	Uniprot
N869	N-Glycosylation	Uniprot
R900	Methylation	Uniprot
Y904	Phosphorylation	Uniprot
N931	N-Glycosylation	Uniprot
T948	Phosphorylation	Uniprot
S955	Phosphorylation	Uniprot

Research Backgrounds

Q9Y4L1_HYOU1_HUMAN

Function:

Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding.

Subcellular Location:

Endoplasmic reticulum lumen.

Tissue Specificity:

Subunit Structure:

Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX.

Family&Domains:

Belongs to the heat shock protein 70 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)

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HYOU1 Antibody - #DF8329