Phospho-HSP20 (Ser16) Antibody - #AF3003
| Product: | Phospho-HSP20 (Ser16) Antibody |
| Catalog: | AF3003 |
| Description: | Rabbit polyclonal antibody to Phospho-HSP20 (Ser16) |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| Mol.Wt.: | 20kDa; 17kD(Calculated). |
| Uniprot: | O14558 |
| RRID: | AB_2834442 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF3003, RRID:AB_2834442.
Fold/Unfold
epididymis luminal protein 55; FLJ32389; Heat shock 20 kDa like protein p20; Heat shock 20 kDa-like protein p20; Heat shock protein alpha crystallin related B6; Heat shock protein beta 6; Heat shock protein beta-6; Heat shock protein, 20-KD; Heat-shock 27-KD protein 6; HEL55; Hsp20; HspB6; HSPB6_HUMAN;
Immunogens
A synthesized peptide derived from human HSP20 around the phosphorylation site of Ser16.
- O14558 HSPB6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEIPVPVQPSWLRRASAPLPGLSAPGRLFDQRFGEGLLEAELAALCPTTLAPYYLRAPSVALPVAQVPTDPGHFSVLLDVKHFSPEEIAVKVVGEHVEVHARHEERPDEHGFVAREFHRRYRLPPGVDPAAVTSALSPEGVLSIQAAPASAQAPPPAAAK
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
Research Backgrounds
Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Seems to have versatile functions in various biological processes. Plays a role in regulating muscle function such as smooth muscle vasorelaxation and cardiac myocyte contractility. May regulate myocardial angiogenesis implicating KDR. Overexpression mediates cardioprotection and angiogenesis after induced damage. Stabilizes monomeric YWHAZ thereby supporting YWHAZ chaperone-like activity.
The N-terminus is blocked.
Phosphorylated at Ser-16 by PKA and probably PKD1K; required to protect cardiomyocytes from apoptosis.
Cytoplasm. Nucleus. Secreted.
Note: Translocates to nuclear foci during heat shock.
Belongs to the small heat shock protein (HSP20) family.
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