ERAP1 Antibody - #DF8347
| Product: | ERAP1 Antibody |
| Catalog: | DF8347 |
| Description: | Rabbit polyclonal antibody to ERAP1 |
| Application: | WB IF/ICC |
| Reactivity: | Human |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 107 kDa; 107kD(Calculated). |
| Uniprot: | Q9NZ08 |
| RRID: | AB_2841613 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF8347, RRID:AB_2841613.
Fold/Unfold
A LAP; A-LAP; Adipocyte derived leucine aminopeptidase; Adipocyte-derived leucine aminopeptidase; ALAP; Aminopeptidase PILS; Aminopeptidase regulator of TNFR1 shedding; APPILS; ARTS 1; ARTS-1; Arts1; Endoplasmic reticulum aminopeptidase 1; Endoplasmic reticulum aminopeptidase associated with antigen processing; ERAAP; ERAAP1; ERAP 1; Erap1; ERAP1_HUMAN; KIAA0525; kiaa0525 protein; PILS AP; PILS-AP; PILSA; PILSAP; Puromycin insensitive leucyl specific aminopeptidase; Puromycin-insensitive leucyl-specific aminopeptidase; Type 1 tumor necrosis factor receptor shedding aminopeptidase regulator; type 1 tumor necrosis factor receptors shedding aminopeptidase regulator; VEGF-induced aminopeptidase;
Immunogens
- Q9NZ08 ERAP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVFLPLKWSLATMSFLLSSLLALLTVSTPSWCQSTEASPKRSDGTPFPWNKIRLPEYVIPVHYDLLIHANLTTLTFWGTTKVEITASQPTSTIILHSHHLQISRATLRKGAGERLSEEPLQVLEHPRQEQIALLAPEPLLVGLPYTVVIHYAGNLSETFHGFYKSTYRTKEGELRILASTQFEPTAARMAFPCFDEPAFKASFSIKIRREPRHLAISNMPLVKSVTVAEGLIEDHFDVTVKMSTYLVAFIISDFESVSKITKSGVKVSVYAVPDKINQADYALDAAVTLLEFYEDYFSIPYPLPKQDLAAIPDFQSGAMENWGLTTYRESALLFDAEKSSASSKLGITMTVAHELAHQWFGNLVTMEWWNDLWLNEGFAKFMEFVSVSVTHPELKVGDYFFGKCFDAMEVDALNSSHPVSTPVENPAQIREMFDDVSYDKGACILNMLREYLSADAFKSGIVQYLQKHSYKNTKNEDLWDSMASICPTDGVKGMDGFCSRSQHSSSSSHWHQEGVDVKTMMNTWTLQKGFPLITITVRGRNVHMKQEHYMKGSDGAPDTGYLWHVPLTFITSKSDMVHRFLLKTKTDVLILPEEVEWIKFNVGMNGYYIVHYEDDGWDSLTGLLKGTHTAVSSNDRASLINNAFQLVSIGKLSIEKALDLSLYLKHETEIMPVFQGLNELIPMYKLMEKRDMNEVETQFKAFLIRLLRDLIDKQTWTDEGSVSERMLRSQLLLLACVHNYQPCVQRAEGYFRKWKESNGNLSLPVDVTLAVFAVGAQSTEGWDFLYSKYQFSLSSTEKSQIEFALCRTQNKEKLQWLLDESFKGDKIKTQEFPQILTLIGRNPVGYPLAWQFLRKNWNKLVQKFELGSSSIAHMVMGTTNQFSTRTRLEEVKGFFSSLKENGSQLRCVQQTIETIEENIGWMDKNFDKIRVWLQSEKLERM
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NZ08 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| N70 | N-Glycosylation | Uniprot | |
| N154 | N-Glycosylation | Uniprot | |
| S179 | Phosphorylation | Uniprot | |
| T185 | Phosphorylation | Uniprot | |
| S268 | Phosphorylation | Uniprot | |
| Y270 | Phosphorylation | Uniprot | |
| Y399 | Phosphorylation | Uniprot | |
| N414 | N-Glycosylation | Uniprot | |
| K440 | Ubiquitination | Uniprot | |
| Y451 | Phosphorylation | Uniprot | |
| K458 | Ubiquitination | Uniprot | |
| Y464 | Phosphorylation | Uniprot | |
| T473 | Phosphorylation | Uniprot | |
| T559 | Phosphorylation | Uniprot | |
| Y608 | Phosphorylation | Uniprot | |
| N760 | N-Glycosylation | Uniprot | |
| K811 | Acetylation | Uniprot |
Research Backgrounds
Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney.
N-glycosylated.
Endoplasmic reticulum membrane>Single-pass type II membrane protein.
Ubiquitous.
Monomer. May also exist as a heterodimer; with ERAP2. Interacts with RBMX.
Belongs to the peptidase M1 family.
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