LHX3 Antibody - #DF8362
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| Product: | LHX3 Antibody |
| Catalog: | DF8362 |
| Description: | Rabbit polyclonal antibody to LHX3 |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus |
| Mol.Wt.: | 43 kDa; 43kD(Calculated). |
| Uniprot: | Q9UBR4 |
| RRID: | AB_2841627 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF8362, RRID:AB_2841627.
Fold/Unfold
CPHD 3; CPHD3; DKFZp762A2013; LHX 3; LHX3; LHX3_HUMAN; LIM 3; LIM homeobox 3; LIM homeobox gene 3; LIM homeobox protein 3; LIM/homeobox protein Lhx3; LIM/homeodomain protein LHX3; Lim3; M2 LHX3; mLim-3; mLIM3; P LIM;
Immunogens
- Q9UBR4 LHX3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLLETGLERDRARPGAAAVCTLGGTREIPLCAGCDQHILDRFILKALDRHWHSKCLKCSDCHTPLAERCFSRGESVYCKDDFFKRFGTKCAACQLGIPPTQVVRRAQDFVYHLHCFACVVCKRQLATGDEFYLMEDSRLVCKADYETAKQREAEATAKRPRTTITAKQLETLKSAYNTSPKPARHVREQLSSETGLDMRVVQVWFQNRRAKEKRLKKDAGRQRWGQYFRNMKRSRGGSKSDKDSVQEGQDSDAEVSFPDEPSLAEMGPANGLYGSLGEPTQALGRPSGALGNFSLEHGGLAGPEQYRELRPGSPYGVPPSPAAPQSLPGPQPLLSSLVYPDTSLGLVPSGAPGGPPPMRVLAGNGPSSDLSTGSSGGYPDFPASPASWLDEVDHAQF
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9UBR4 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T63 | Phosphorylation | Uniprot | |
| S71 | Phosphorylation | Uniprot | |
| R159 | Methylation | Uniprot | |
| R161 | Methylation | Uniprot | |
| S174 | Phosphorylation | Uniprot | |
| Y176 | Phosphorylation | Uniprot | |
| Y227 | Phosphorylation | Uniprot | |
| S234 | Phosphorylation | Uniprot | |
| S238 | Phosphorylation | Uniprot |
Research Backgrounds
Acts as a transcriptional activator. Binds to and activates the promoter of the alpha-glycoprotein gene, and synergistically enhances transcription from the prolactin promoter in cooperation with POU1F1/Pit-1 (By similarity). Required for the establishment of the specialized cells of the pituitary gland and the nervous system. Involved in the development of interneurons and motor neurons in cooperation with LDB1 and ISL1.
Nucleus.
Interacts with POU1F1. At neuronal promoters, interacts with LDB1, in motor neurons LDB1 is displaced by ISL1 and a ternary complex is formed in which ISL1 contacts both LHX3 and LDB1 (By similarity).
The LIM domain specifically interacts with the Pit-1 POU domain and is required for synergistic interactions with Pit-1, but not for basal transcriptional activation events.
References
Application: IF/ICC Species: Mouse Sample: MTEC cells
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