CHD5 Antibody - #DF8374
| Product: | CHD5 Antibody |
| Catalog: | DF8374 |
| Description: | Rabbit polyclonal antibody to CHD5 |
| Application: | WB IF/ICC |
| Reactivity: | Human |
| Prediction: | Pig, Bovine, Horse, Sheep, Dog |
| Mol.Wt.: | 223 kDa; 223kD(Calculated). |
| Uniprot: | Q8TDI0 |
| RRID: | AB_2841636 |
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF8374, RRID:AB_2841636.
Fold/Unfold
ATP-dependent helicase CHD5; CHD 5; CHD-5; CHD5; CHD5_HUMAN; Chromodomain-helicase-DNA-binding protein 5; DKFZp434N231; KIAA0444; RP1-233K16.2;
Immunogens
Preferentially expressed in total brain, fetal brain, and cerebellum. It is also moderately expressed in the adrenal gland and detected in testis.
- Q8TDI0 CHD5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRGPVGTEEELPRLFAEEMENEDEMSEEEDGGLEAFDDFFPVEPVSLPKKKKPKKLKENKCKGKRKKKEGSNDELSENEEDLEEKSESEGSDYSPNKKKKKKLKDKKEKKAKRKKKDEDEDDNDDGCLKEPKSSGQLMAEWGLDDVDYLFSEEDYHTLTNYKAFSQFLRPLIAKKNPKIPMSKMMTVLGAKWREFSANNPFKGSSAAAAAAAVAAAVETVTISPPLAVSPPQVPQPVPIRKAKTKEGKGPGVRKKIKGSKDGKKKGKGKKTAGLKFRFGGISNKRKKGSSSEEDEREESDFDSASIHSASVRSECSAALGKKSKRRRKKKRIDDGDGYETDHQDYCEVCQQGGEIILCDTCPRAYHLVCLDPELEKAPEGKWSCPHCEKEGIQWEPKDDDDEEEEGGCEEEEDDHMEFCRVCKDGGELLCCDACPSSYHLHCLNPPLPEIPNGEWLCPRCTCPPLKGKVQRILHWRWTEPPAPFMVGLPGPDVEPSLPPPKPLEGIPEREFFVKWAGLSYWHCSWVKELQLELYHTVMYRNYQRKNDMDEPPPFDYGSGDEDGKSEKRKNKDPLYAKMEERFYRYGIKPEWMMIHRILNHSFDKKGDVHYLIKWKDLPYDQCTWEIDDIDIPYYDNLKQAYWGHRELMLGEDTRLPKRLLKKGKKLRDDKQEKPPDTPIVDPTVKFDKQPWYIDSTGGTLHPYQLEGLNWLRFSWAQGTDTILADEMGLGKTVQTIVFLYSLYKEGHSKGPYLVSAPLSTIINWEREFEMWAPDFYVVTYTGDKESRSVIRENEFSFEDNAIRSGKKVFRMKKEVQIKFHVLLTSYELITIDQAILGSIEWACLVVDEAHRLKNNQSKFFRVLNSYKIDYKLLLTGTPLQNNLEELFHLLNFLTPERFNNLEGFLEEFADISKEDQIKKLHDLLGPHMLRRLKADVFKNMPAKTELIVRVELSQMQKKYYKFILTRNFEALNSKGGGNQVSLLNIMMDLKKCCNHPYLFPVAAVEAPVLPNGSYDGSSLVKSSGKLMLLQKMLKKLRDEGHRVLIFSQMTKMLDLLEDFLEYEGYKYERIDGGITGGLRQEAIDRFNAPGAQQFCFLLSTRAGGLGINLATADTVIIYDSDWNPHNDIQAFSRAHRIGQNKKVMIYRFVTRASVEERITQVAKRKMMLTHLVVRPGLGSKSGSMTKQELDDILKFGTEELFKDDVEGMMSQGQRPVTPIPDVQSSKGGNLAASAKKKHGSTPPGDNKDVEDSSVIHYDDAAISKLLDRNQDATDDTELQNMNEYLSSFKVAQYVVREEDGVEEVEREIIKQEENVDPDYWEKLLRHHYEQQQEDLARNLGKGKRIRKQVNYNDASQEDQEWQDELSDNQSEYSIGSEDEDEDFEERPEGQSGRRQSRRQLKSDRDKPLPPLLARVGGNIEVLGFNARQRKAFLNAIMRWGMPPQDAFNSHWLVRDLRGKSEKEFRAYVSLFMRHLCEPGADGAETFADGVPREGLSRQHVLTRIGVMSLVRKKVQEFEHVNGKYSTPDLIPEGPEGKKSGEVISSDPNTPVPASPAHLLPAPLGLPDKMEAQLGYMDEKDPGAQKPRQPLEVQALPAALDRVESEDKHESPASKERAREERPEETEKAPPSPEQLPREEVLPEKEKILDKLELSLIHSRGDSSELRPDDTKAEEKEPIETQQNGDKEEDDEGKKEDKKGKFKFMFNIADGGFTELHTLWQNEERAAVSSGKIYDIWHRRHDYWLLAGIVTHGYARWQDIQNDPRYMILNEPFKSEVHKGNYLEMKNKFLARRFKLLEQALVIEEQLRRAAYLNMTQDPNHPAMALNARLAEVECLAESHQHLSKESLAGNKPANAVLHKVLNQLEELLSDMKADVTRLPSMLSRIPPVAARLQMSERSILSRLTNRAGDPTIQQGAFGSSQMYSNNFGPNFRGPGPGGIVNYNQMPLGPYVTDI
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8TDI0 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S86 | Phosphorylation | Uniprot | |
| K100 | Acetylation | Uniprot | |
| K102 | Acetylation | Uniprot | |
| K106 | Acetylation | Uniprot | |
| K107 | Acetylation | Uniprot | |
| K174 | Acetylation | Uniprot | |
| K175 | Acetylation | Uniprot | |
| T340 | Phosphorylation | Uniprot | |
| Y556 | Phosphorylation | Uniprot | |
| S558 | Phosphorylation | Uniprot | |
| S565 | Phosphorylation | Uniprot | |
| K588 | Sumoylation | Uniprot | |
| K588 | Ubiquitination | Uniprot | |
| Y610 | Phosphorylation | Uniprot | |
| K858 | Acetylation | Uniprot | |
| K858 | Ubiquitination | Uniprot | |
| K918 | Ubiquitination | Uniprot | |
| K933 | Ubiquitination | Uniprot | |
| K938 | Ubiquitination | Uniprot | |
| K943 | Ubiquitination | Uniprot | |
| K958 | Methylation | Uniprot | |
| K961 | Methylation | Uniprot | |
| S1047 | Phosphorylation | Uniprot | |
| Y1062 | Phosphorylation | Uniprot | |
| T1169 | Phosphorylation | Uniprot | |
| K1194 | Ubiquitination | Uniprot | |
| T1217 | Phosphorylation | Uniprot | |
| K1237 | Acetylation | Uniprot | |
| K1310 | Sumoylation | Uniprot | |
| K1310 | Ubiquitination | Uniprot | |
| Y1319 | Phosphorylation | Uniprot | |
| K1322 | Ubiquitination | Uniprot | |
| R1325 | Methylation | Uniprot | |
| K1341 | Ubiquitination | Uniprot | |
| Q1390 | Methylation | Uniprot | |
| K1406 | Sumoylation | Uniprot | |
| K1406 | Ubiquitination | Uniprot | |
| K1430 | Ubiquitination | Uniprot | |
| R1465 | Methylation | Uniprot | |
| R1511 | Methylation | Uniprot | |
| S1631 | Phosphorylation | Uniprot | |
| S1654 | Phosphorylation | Uniprot | |
| S1658 | Phosphorylation | Uniprot | |
| T1670 | Phosphorylation | Uniprot | |
| R1755 | Methylation | Uniprot | |
| S1846 | Phosphorylation | Uniprot | |
| K1851 | Ubiquitination | Uniprot | |
| S1869 | Phosphorylation | Uniprot | |
| K1872 | Ubiquitination | Uniprot | |
| T1876 | Phosphorylation | Uniprot | |
| S1895 | Phosphorylation | Uniprot |
Research Backgrounds
Chromatin-remodeling protein that binds DNA through histones and regulates gene transcription. May specifically recognize and bind trimethylated 'Lys-27' (H3K27me3) and non-methylated 'Lys-4' of histone H3. Plays a role in the development of the nervous system by activating the expression of genes promoting neuron terminal differentiation. In parallel, it may also positively regulate the trimethylation of histone H3 at 'Lys-27' thereby specifically repressing genes that promote the differentiation into non-neuronal cell lineages. Tumor suppressor, it regulates the expression of genes involved in cell proliferation and differentiation. Downstream activated genes may include CDKN2A that positively regulates the p53/TP53 pathway, which in turn, prevents cell proliferation. In spermatogenesis, it probably regulates histone hyperacetylation and the replacement of histones by transition proteins in chromatin, a crucial step in the condensation of spermatid chromatin and the production of functional spermatozoa.
Methylated at Gln-1390 by N6AMT1.
Nucleus.
Note: Associates with heterochromatin.
Preferentially expressed in total brain, fetal brain, and cerebellum. It is also moderately expressed in the adrenal gland and detected in testis.
May be part of a nucleosome remodeling and histone deacetylation, NuRD-like, complex composed at least of GATAD2B, HDAC1, HDAC2 and MTA3.
The PHD domains mediate specific binding to histone H3 unmethylated at 'Lys-4' and may preferentially recruit the protein to transcriptionally inactive genes.
The chromo domains mediate specific binding to histone H3 trimethylated at 'Lys-27' (H3K27me3) and may be required in neuron differentiation for proper gene regulation.
Belongs to the SNF2/RAD54 helicase family.
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