TLS/FUS Antibody - #DF8391

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Product: TLS/FUS Antibody
Catalog: DF8391
Description: Rabbit polyclonal antibody to TLS/FUS
Application: WB IHC IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 70kD; 53kD(Calculated).
Uniprot: P35637
RRID: AB_2841647

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Rabbit(92%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
TLS/FUS Antibody detects endogenous levels of total TLS/FUS.
RRID:
AB_2841647
Cite Format: Affinity Biosciences Cat# DF8391, RRID:AB_2841647.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

75 kDa DNA pairing protein; 75 kDa DNA-pairing protein; ALS6; Amyotrophic lateral sclerosis 6; fus; FUS CHOP; Fus like protein; FUS_HUMAN; FUS1; Fused in sarcoma; Fusion (involved in t(12;16) in malignant liposarcoma); Fusion derived from t(12;16) malignant liposarcoma; Fusion gene in myxoid liposarcoma; Heterogeneous nuclear ribonucleoprotein P2; hnRNP P2; hnRNPP2; Oncogene FUS; Oncogene TLS; POMp75; RNA binding protein FUS; RNA-binding protein FUS; TLS; TLS CHOP; Translocated in liposarcoma; Translocated in liposarcoma protein;

Immunogens

Immunogen:

A synthesized peptide derived from human TLS/FUS, corresponding to a region within C-terminal amino acids.

Uniprot:

P35637(FUS_HUMAN) >>Visit HPA database.

Gene(ID):
FUS(2521)
Expression:
P35637 FUS_HUMAN:

Ubiquitous.

Sequence:
MASNDYTQQATQSYGAYPTQPGQGYSQQSSQPYGQQSYSGYSQSTDTSGYGQSSYSSYGQSQNTGYGTQSTPQGYGSTGGYGSSQSSQSSYGQQSSYPGYGQQPAPSSTSGSYGSSSQSSSYGQPQSGSYSQQPSYGGQQQSYGQQQSYNPPQGYGQQNQYNSSSGGGGGGGGGGNYGQDQSSMSSGGGSGGGYGNQDQSGGGGSGGYGQQDRGGRGRGGSGGGGGGGGGGYNRSSGGYEPRGRGGGRGGRGGMGGSDRGGFNKFGGPRDQGSRHDSEQDNSDNNTIFVQGLGENVTIESVADYFKQIGIIKTNKKTGQPMINLYTDRETGKLKGEATVSFDDPPSAKAAIDWFDGKEFSGNPIKVSFATRRADFNRGGGNGRGGRGRGGPMGRGGYGGGGSGGGGRGGFPSGGGGGGGQQRAGDWKCPNPTCENMNFSWRNECNQCKAPKPDGPGGGPGGSHMGGNYGDDRRGGRGGYDRGGYRGRGGDRGGFRGGRGGGDRGGFGPGKMDSRGEHRQDRRERPY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
92
Horse
0
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

DNA/RNA-binding protein that plays a role in various cellular processes such as transcription regulation, RNA splicing, RNA transport, DNA repair and damage response. Binds to nascent pre-mRNAs and acts as a molecular mediator between RNA polymerase II and U1 small nuclear ribonucleoprotein thereby coupling transcription and splicing. Binds also its own pre-mRNA and autoregulates its expression; this autoregulation mechanism is mediated by non-sense-mediated decay. Plays a role in DNA repair mechanisms by promoting D-loop formation and homologous recombination during DNA double-strand break repair. In neuronal cells, plays crucial roles in dendritic spine formation and stability, RNA transport, mRNA stability and synaptic homeostasis (By similarity).

PTMs:

Arg-216 and Arg-218 are dimethylated, probably to asymmetric dimethylarginine.

Phosphorylated in its N-terminal serine residues upon induced DNA damage. ATM and DNA-PK are able to phosphorylate FUS N-terminal region.

Subcellular Location:

Nucleus.
Note: Displays a punctate pattern inside the nucleus and is excluded from nucleoli.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Family&Domains:

Belongs to the RRM TET family.

Research Fields

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

References

1). Cancer-associated fibroblast-derived exosomal miR-18b promotes breast cancer invasion and metastasis by regulating TCEAL7. Cell death & disease, 2021 (PubMed: 34853307) [IF=8.1]

Application: WB    Species: human    Sample: breast cancer cells

Fig. 3: The upregulated expression of miR-18b in exosomes enhanced migration and invasion of breast cancer cells. A qRT-PCR detected miR-18b expression in cancer cells co-cultured with NFs and CAFs-derived exosomes (n = 3). B, C Transwell experiments demonstrated the effect of miR-18b on the ability of breast cancer cells in migration and invasion (n = 3). Scale bar, 50 μm. D RBPDB analysis was used to predict the specific interaction between the miR-18b sequence and RBP motifs (threshold 0.7). E Using real-time PCR to measure the expression of miR-18b in CAFs-derived exosomes transfected with specific siRNA targeting FUS, ACO1, or YTHDC1 (n = 3). F Real-time PCR analysis detected the expression of miR-18b after silencing FUS in CAFs (n = 3). G The miRNA pull-down experiment analyzed the interaction of miR-18b and miR-18b mutant with FUS in the cytoplasm, nucleus, and exosomal lysates of CAFs, biotinylated poly. H As a negative control (n = 3). I Anti-FUS antibody (or IgG as a control) was used for RIP detection of CAFs cells or exosomal lysates (n = 3). * indicates p 
2). FAIRE-MS reveals mitotic retention of transcriptional regulators on a proteome-wide scale. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2023 (PubMed: 36583687) [IF=4.4]

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