RASSF2 Antibody | Affinity Biosciences

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Product:	RASSF2 Antibody
Catalog:	DF8437
Description:	Rabbit polyclonal antibody to RASSF2
Application:	WB IHC IF/ICC
Cited expt.:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	37 kDa; 38kD(Calculated).
Uniprot:	P50749
RRID:	AB_2841683

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

RASSF2 Antibody detects endogenous levels of total RASSF2.

RRID:

AB_2841683
Cite Format: Affinity Biosciences Cat# DF8437, RRID:AB_2841683.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CENP 34; Centromere protein 34; Ras association (RalGDS/AF 6) domain family 2; Ras association (RalGDS/AF-6) domain family member 2; Ras association domain containing protein 2; Ras association domain-containing protein 2; RASF2_HUMAN; RASFADIN; RASSF2; RP4-599I11.1;

Immunogens

Immunogen:

A synthesized peptide derived from human RASSF2, corresponding to a region within the internal amino acids.

Uniprot:

P50749(RASF2_HUMAN) >>Visit HPA database.

Gene(ID):

RASSF2(9770)

Expression:

P50749 RASF2_HUMAN:

Widely expressed with highest levels in brain, placenta, peripheral blood and lung. Frequently down-regulated in lung tumor cell lines.

Sequence:

P50749 RASF2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDYSHQTSLVPCGQDKYISKNELLLHLKTYNLYYEGQNLQLRHREEEDEFIVEGLLNISWGLRRPIRLQMQDDNERIRPPPSSSSWHSGCNLGAQGTTLKPLTVPKVQISEVDAPPEGDQMPSSTDSRGLKPLQEDTPQLMRTRSDVGVRRRGNVRTPSDQRRIRRHRFSINGHFYNHKTSVFTPAYGSVTNVRINSTMTTPQVLKLLLNKFKIENSAEEFALYVVHTSGEKQKLKATDYPLIARILQGPCEQISKVFLMEKDQVEEVTYDVAQYIKFEMPVLKSFIQKLQEEEDREVKKLMRKYTVLRLMIRQRLEEIAETPATI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Sheep

100

Dog

100

Xenopus

100

Rabbit

100

Bovine

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

P50749_RASF2_HUMAN

Function:

Potential tumor suppressor. Acts as a KRAS-specific effector protein. May promote apoptosis and cell cycle arrest. Stabilizes STK3/MST2 by protecting it from proteasomal degradation.

PTMs:

Phosphorylated by STK3/MST2 and STK4/MST1.

Subcellular Location:

Nucleus. Cytoplasm. Chromosome>Centromere>Kinetochore.
Note: Translocates to the cytoplasm in the presence of STK3/MST2 AND STK4/MST1.

Tissue Specificity:

Widely expressed with highest levels in brain, placenta, peripheral blood and lung. Frequently down-regulated in lung tumor cell lines.

Research Fields

· Environmental Information Processing > Signal transduction > Hippo signaling pathway - multiple species. (View pathway)

References

1). MiR-93-5p promotes gastric cancer-cell progression via inactivation of the Hippo signaling pathway. GENE, 2018 (PubMed: 29045821) [IF=2.6]

Application: WB Species: human Sample:

Figure 4. FAT4 and LATS2 are direct miR-93-5p targets. (A) Quantitative PCR results showed that miR-93-5p overexpression suppressed FAT4 and LATS2 mRNA expression in SGC-7901 cells, while conversely, the opposite result was observed in HGC-27 cells exhibiting downregulated miR-93-5p expression. RUNX3 and RASSF2 mRNA levels were unchanged in response to either up- or downregulation of miR-93-5p. (B) Western blot analyses of FAT4, LATS2, RUNX3, and RASSF2 expression after transfection with miR-93-5p mimics and/or the miR-93-5p inhibitor.

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For Research Use Only.
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RASSF2 Antibody - #DF8437