Ameloblastin Antibody - #DF8503

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Product: Ameloblastin Antibody
Catalog: DF8503
Description: Rabbit polyclonal antibody to Ameloblastin
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Rabbit
Mol.Wt.: 48 kDa; 48kD(Calculated).
Uniprot: Q9NP70
RRID: AB_2841709

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Rabbit(100%)
Clonality:
Polyclonal
Specificity:
Ameloblastin Antibody detects endogenous levels of total Ameloblastin.
RRID:
AB_2841709
Cite Format: Affinity Biosciences Cat# DF8503, RRID:AB_2841709.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AMBN; AMBN_HUMAN; Ameloblastin (enamel matrix protein); Ameloblastin;

Immunogens

Immunogen:
Uniprot:

Q9NP70(AMBN_HUMAN) >>Visit HPA database.

Gene(ID):
AMBN(258)
Expression:
Q9NP70 AMBN_HUMAN:

Ameloblast-specific. Located at the Tomes processes of secretory ameloblasts and in the sheath space between rod-interrod enamel.

Sequence:
MSASKIPLFKMKDLILILCLLEMSFAVPFFPQQSGTPGMASLSLETMRQLGSLQRLNTLSQYSRYGFGKSFNSLWMHGLLPPHSSLPWMRPREHETQQYEYSLPVHPPPLPSQPSLKPQQPGLKPFLQSAAATTNQATALKEALQPPIHLGHLPLQEGELPLVQQQVAPSDKPPKPELPGVDFADPQGPSLPGMDFPDPQGPSLPGLDFADPQGSTIFQIARLISHGPMPQNKQSPLYPGMLYVPFGANQLNAPARLGIMSSEEVAGGREDPMAYGAMFPGFGGMRPGFEGMPHNPAMGGDFTLEFDSPVAATKGPENEEGGAQGSPMPEANPDNLENPAFLTELEPAPHAGLLALPKDDIPGLPRSPSGKMKGLPSVTPAAADPLMTPELADVYRTYDADMTTSVDFQEEATMDTTMAPNSLQTSMPGNKAQEPEMMHDAWHFQEP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
70
Bovine
67
Dog
67
Sheep
58
Xenopus
50
Pig
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NP70 As Substrate

Site PTM Type Enzyme
Y395 Phosphorylation

Research Backgrounds

Function:

Involved in the mineralization and structural organization of enamel.

Subcellular Location:

Secreted>Extracellular space>Extracellular matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ameloblast-specific. Located at the Tomes processes of secretory ameloblasts and in the sheath space between rod-interrod enamel.

Family&Domains:

Belongs to the ameloblastin family.

References

1). Comprehensive Analysis Identifies Ameloblastin-Related Competitive Endogenous RNA as a Prognostic Biomarker for Testicular Germ Cell Tumour. Cancers, 2022 (PubMed: 35454778) [IF=5.2]

Application: IF/ICC    Species: Rat    Sample: testicular tissue

Figure 1. Low mRNA expression of AMBN in TGCT and its clinical implication. (A) Distribution of AMBN expression in normal testis and TGCT. (B) AMBN expression in rat normal testicular tissue (40×, anti-AMBN (yellow), anti-vimentin (magenta) and DRAQ5 (cyan) to label the nuclei.) (C) Receiver operating characteristic (ROC) curve of AMBN in TGCT (D) AMBN mRNA expression in different TGCT stages (E–G) Kaplan-Meier survival curves were used to compare the difference in progress free interval (PFI) between low and high mRNA expression of AMBN under different conditions (H) OncoPrint plot showing deletion mutations in AMBN gene in TGCT.
2). Revisiting ameloblastin; addressing the EMT-ECM axis above and beyond oral biology. Frontiers in Cell and Developmental Biology, 2023 (PubMed: 38020879)

Application: IHC    Species: Human    Sample:

FIGURE 1 Immunofluorescence imaging of various GI tract tissues. Upper grayscale panels show overview of the tissue in the region where the AMBN signal was detected. Scale bar 200 μm. Lower panels show detection of AMBN immunostaining shown in ImageJ lookup table “red hot” and tissue autofluorescence in cyan. Scale bar 20 μm. GI tract overview diagram was created in Biorender.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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