ATP7A Antibody | Affinity Biosciences

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ATP7A Antibody - DF8506 staining A549 cells by IF/ICC.

ATP7A Antibody - Figure 7 Expression of the ATP7A and ATP7B proteins in MD and WD fibroblasts.

ATP7A Antibody - Fig.

Product:	ATP7A Antibody
Catalog:	DF8506
Description:	Rabbit polyclonal antibody to ATP7A
Application:	WB IHC IF/ICC
Cited expt.:	WB, IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	163 kDa; 163kD(Calculated).
Uniprot:	Q04656
RRID:	AB_2841712

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(87%), Horse(93%), Sheep(87%), Rabbit(100%), Dog(93%)

Clonality:

Polyclonal

Specificity:

ATP7A Antibody detects endogenous levels of total ATP7A.

RRID:

AB_2841712
Cite Format: Affinity Biosciences Cat# DF8506, RRID:AB_2841712.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ATP 7A; ATP7A; ATP7A_HUMAN; ATPase copper transporting alpha polypeptide; ATPase Cu++ transporting alpha polypeptide (Menkes syndrome); ATPase Cu++ transporting alpha polypeptide; Copper pump 1; Copper transporting ATPase 1; Copper-transporting ATPase 1; Cu++ transporting P type ATPase; DSMAX; FLJ17790; MC 1; MC1; Menkes disease associated protein; Menkes disease-associated protein; Menkes syndrome; MK; MNK; OHS; OTTHUMP00000062077; SMAX3;

Immunogens

Immunogen:

A synthesized peptide derived from human ATP7A, corresponding to a region within C-terminal amino acids.

Uniprot:

Q04656(ATP7A_HUMAN) >>Visit HPA database.

Gene(ID):

ATP7A(538)

Expression:

Q04656 ATP7A_HUMAN:

Found in most tissues except liver. Isoform 3 is widely expressed including in liver cell lines. Isoform 1 is expressed in fibroblasts, choriocarcinoma, colon carcinoma and neuroblastoma cell lines. Isoform 2 is expressed in fibroblasts, colon carcinoma and neuroblastoma cell lines.

Sequence:

Q04656 ATP7A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDPSMGVNSVTISVEGMTCNSCVWTIEQQIGKVNGVHHIKVSLEEKNATIIYDPKLQTPKTLQEAIDDMGFDAVIHNPDPLPVLTDTLFLTVTASLTLPWDHIQSTLLKTKGVTDIKIYPQKRTVAVTIIPSIVNANQIKELVPELSLDTGTLEKKSGACEDHSMAQAGEVVLKMKVEGMTCHSCTSTIEGKIGKLQGVQRIKVSLDNQEATIVYQPHLISVEEMKKQIEAMGFPAFVKKQPKYLKLGAIDVERLKNTPVKSSEGSQQRSPSYTNDSTATFIIDGMHCKSCVSNIESTLSALQYVSSIVVSLENRSAIVKYNASSVTPESLRKAIEAVSPGLYRVSITSEVESTSNSPSSSSLQKIPLNVVSQPLTQETVINIDGMTCNSCVQSIEGVISKKPGVKSIRVSLANSNGTVEYDPLLTSPETLRGAIEDMGFDATLSDTNEPLVVIAQPSSEMPLLTSTNEFYTKGMTPVQDKEEGKNSSKCYIQVTGMTCASCVANIERNLRREEGIYSILVALMAGKAEVRYNPAVIQPPMIAEFIRELGFGATVIENADEGDGVLELVVRGMTCASCVHKIESSLTKHRGILYCSVALATNKAHIKYDPEIIGPRDIIHTIESLGFEASLVKKDRSASHLDHKREIRQWRRSFLVSLFFCIPVMGLMIYMMVMDHHFATLHHNQNMSKEEMINLHSSMFLERQILPGLSVMNLLSFLLCVPVQFFGGWYFYIQAYKALKHKTANMDVLIVLATTIAFAYSLIILLVAMYERAKVNPITFFDTPPMLFVFIALGRWLEHIAKGKTSEALAKLISLQATEATIVTLDSDNILLSEEQVDVELVQRGDIIKVVPGGKFPVDGRVIEGHSMVDESLITGEAMPVAKKPGSTVIAGSINQNGSLLICATHVGADTTLSQIVKLVEEAQTSKAPIQQFADKLSGYFVPFIVFVSIATLLVWIVIGFLNFEIVETYFPGYNRSISRTETIIRFAFQASITVLCIACPCSLGLATPTAVMVGTGVGAQNGILIKGGEPLEMAHKVKVVVFDKTGTITHGTPVVNQVKVLTESNRISHHKILAIVGTAESNSEHPLGTAITKYCKQELDTETLGTCIDFQVVPGCGISCKVTNIEGLLHKNNWNIEDNNIKNASLVQIDASNEQSSTSSSMIIDAQISNALNAQQYKVLIGNREWMIRNGLVINNDVNDFMTEHERKGRTAVLVAVDDELCGLIAIADTVKPEAELAIHILKSMGLEVVLMTGDNSKTARSIASQVGITKVFAEVLPSHKVAKVKQLQEEGKRVAMVGDGINDSPALAMANVGIAIGTGTDVAIEAADVVLIRNDLLDVVASIDLSRKTVKRIRINFVFALIYNLVGIPIAAGVFMPIGLVLQPWMGSAAMAASSVSVVLSSLFLKLYRKPTYESYELPARSQIGQKSPSEISVHVGIDDTSRNSPKLGLLDRIVNYSRASINSLLSDKRSLNSVVTSEPDKHSLLVGDFREDDDTAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Horse

Dog

Bovine

Sheep

Zebrafish

Xenopus

Pig

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Q04656_ATP7A_HUMAN

Function:

May supply copper to copper-requiring proteins within the secretory pathway, when localized in the trans-Golgi network. Under conditions of elevated extracellular copper, it relocalized to the plasma membrane where it functions in the efflux of copper from cells.

Subcellular Location:

Golgi apparatus>trans-Golgi network membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein.
Note: Cycles constitutively between the trans-Golgi network (TGN) and the plasma membrane (PubMed:9147644). Predominantly found in the TGN and relocalized to the plasma membrane in response to elevated copper levels.

Cytoplasm>Cytosol.

Endoplasmic reticulum.

Tissue Specificity:

Family&Domains:

The C-terminal di-leucine, 1487-Leu-Leu-1488, is an endocytic targeting signal which functions in retrieving recycling from the plasma membrane to the TGN. Mutation of the di-leucine signal results in the accumulation of the protein in the plasma membrane.

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily.

Research Fields

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Organismal Systems > Digestive system > Mineral absorption.

References

1). Metformin-based carbon dots based on biguanide functional groups for simultaneous chelation of copper ions and inhibitable colorectal cancer therapy. Carbon, 2023 [IF=10.5]

2). Response of Fibroblasts from Menkes' and Wilson's Copper Metabolism-Related Disorders to Ionizing Radiation: Influence of the Nucleo-Shuttling of the ATM Protein Kinase. Biomolecules, 2023 (PubMed: 38136617) [IF=5.5]

Application: WB Species: Human Sample: fibroblasts

Figure 7 Expression of the ATP7A and ATP7B proteins in MD and WD fibroblasts. Anti-ATP7A and anti-ATP7B immunoblots with total or cytoplasmic protein extracts were applied to the indicated non-irradiated control Hs27, MD (A,B), and WD (C,D) fibroblasts. The grey levels corresponding to each condition are shown in Figure S4. Original western blot images can be found in raw data.

3). Copper transporter gene ATP7A: A predictive biomarker for immunotherapy and targeted therapy in hepatocellular carcinoma. International immunopharmacology, 2023 (PubMed: 36502594) [IF=4.8]

Application: IHC Species: Human Sample: HCC tissues

Fig. 5. ATP7A is associated with CD3 + T cells and CD8 + T cells in HCC. (A) Immunohistochemical analysis of ATP7A and CD3, CD8 expression in HCC tissues. (B-E) Association between ATP7A expression and CD3, CD8 expression in HCC patients. ***, P < 0.001.

4). Cancer-associated fibroblasts promote oral squamous cell carcinoma progression by targeting ATP7A via exosome-mediated paracrine miR-148b-3p. Cellular signalling, 2025 (PubMed: 39884640) [IF=4.4]

5). Curcumin suppresses copper accumulation in non-small cell lung cancer by binding ATOX1. BMC pharmacology & toxicology, 2024 (PubMed: 39169392) [IF=2.9]

Application: WB Species: human Sample: A549 and H1299 cells

Fig. 1 Effects of curcumin on ATOX1-associated copper chaperones in A549 and H1299 cells. (A) The binding activity between curcumin and ATOX1 protein was analyzed using molecular docking. (B) Cell viability was measured by CCK-8. (C) Levels of ATOX1, ATP7A, and COX17 proteins were measured by western blotting. The levels of proteins were normalized by β-actin. The full-length blots were shown in Supplementary Fig. 1 and Fig. 2. (D) The semi-quantitative analysis of the blots was analyzed by ImageJ software. (E) Effects of DC-AC50 treatment on cell viability. Three parallel experiments were performed in cells. NS no significance. *p

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ATP7A Antibody - #DF8506