Calcitonin Antibody - #DF8512

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Product: Calcitonin Antibody
Catalog: DF8512
Description: Rabbit polyclonal antibody to Calcitonin
Application: WB IHC IF/ICC
Cited expt.: IHC
Reactivity: Human, Mouse, Rat
Prediction: Dog, Chicken
Mol.Wt.: 13 kDa; 14kD,15kD(Calculated).
Uniprot: P06881 | P01258
RRID: AB_2841717

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
Calcitonin Antibody detects endogenous levels of total Calcitonin.
RRID:
AB_2841717
Cite Format: Affinity Biosciences Cat# DF8512, RRID:AB_2841717.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Calcitonin/calcitonin related polypeptide alpha; Alpha type CGRP; Beta type CGRP; CALC1; CALC2; CALCA; CALCB; Calcitonin 1; Calcitonin 2; Calcitonin gene related peptide I; Calcitonin gene related peptide II; Calcitonin related polypeptide alpha; Calcitonin related polypeptide beta; CGRP; CGRP I; CGRP II; CGRP1; CGRP2; CT; Katacalcin; KC; Alpha CGRP; alpha type CGRP; CALC 1; CALC A; CALC1; calcitonin 1; calcitonin gene related peptide 1; calcitonin gene related peptide I; calcitonin related polypeptide alpha; CGRP 1; CGRP; CGRP I; CGRP1; CT; katacalcin; KC;

Immunogens

Immunogen:

A synthesized peptide derived from human Calcitonin, corresponding to a region within the internal amino acids.

Uniprot:

P06881(CALCA_HUMAN) >>Visit HPA database.

P01258(CALC_HUMAN) >>Visit HPA database.

Gene(ID):
CALCA(796)
Sequence:
MGFQKFSPFLALSILVLLQAGSLHAAPFRSALESSPADPATLSEDEARLLLAALVQDYVQMKASELEQEQEREGSRIIAQKRACDTATCVTHRLAGLLSRSGGVVKNNFVPTNVGSKAFGRRRRDLQA

MGFQKFSPFLALSILVLLQAGSLHAAPFRSALESSPADPATLSEDEARLLLAALVQDYVQMKASELEQEQEREGSSLDSPRSKRCGNLSTCMLGTYTQDFNKFHTFPQTAIGVGAPGKKRDMSSDLERDHRPHVSMPQNAN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Chicken
100
Pig
75
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

CGRP induces vasodilation. It dilates a variety of vessels including the coronary, cerebral and systemic vasculature. Its abundance in the CNS also points toward a neurotransmitter or neuromodulator role. It also elevates platelet cAMP.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the calcitonin family.

Function:

Calcitonin causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.

Katacalcin is a potent plasma calcium-lowering peptide.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the calcitonin family.

References

1). Partially brain effects of injection of human umbilical cord mesenchymal stem cells at injury sites in a mouse model of thoracic spinal cord contusion. Frontiers in molecular neuroscience, 2023 (PubMed: 37342099) [IF=3.5]

Application: IHC    Species: Mouse    Sample:

Figure 5. (A–D) Immunohistochemistry was used to detect the expression of CGRP, Substance P, C-Fos and KCC2 in the anterior cingulate cortex (ACC). (E1–E4) The integrated optical density (IOD) was quantitatively analyzed (IOD/area) to determine marker expression. The expression of CGRP, Substance P and C-Fos in the ACC in the SCI model increased, and the expression of KCC2 decreased (one-way ANOVA; SCI vs. sham, **P < 0.0001). The expression of CGRP, Substance P and C-Fos decreased, whereas that of KCC2 increased, after local injection of HU-MSCs (one-way ANOVA; SCI + HU-MSC vs. SCI/SCI + PBS, **P < 0.0001).

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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