Product: Involucrin Antibody
Catalog: AF0186
Description: Rabbit polyclonal antibody to Involucrin
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Mol.Wt.: 68~120kDa; 68kD(Calculated).
Uniprot: P07476
RRID: AB_2833379

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
Involucrin Antibody detects endogenous levels of total Involucrin.
RRID:
AB_2833379
Cite Format: Affinity Biosciences Cat# AF0186, RRID:AB_2833379.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

INVO_HUMAN; Involucrin; IVL;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P07476 INVO_HUMAN:

Keratinocytes of epidermis and other stratified squamous epithelia.

Description:
IVL Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia. Belongs to the involucrin family. Directly or indirectly cross-linked to cornifelin (CNFN).
Sequence:
MSQQHTLPVTLSPALSQELLKTVPPPVNTHQEQMKQPTPLPPPCQKVPVELPVEVPSKQEEKHMTAVKGLPEQECEQQQKEPQEQELQQQHWEQHEEYQKAENPEQQLKQEKTQRDQQLNKQLEEEKKLLDQQLDQELVKRDEQLGMKKEQLLELPEQQEGHLKHLEQQEGQLKHPEQQEGQLELPEQQEGQLELPEQQEGQLELPEQQEGQLELPEQQEGQLELPEQQEGQLELPQQQEGQLELSEQQEGQLELSEQQEGQLKHLEHQEGQLEVPEEQMGQLKYLEQQEGQLKHLDQQEKQPELPEQQMGQLKHLEQQEGQPKHLEQQEGQLEQLEEQEGQLKHLEQQEGQLEHLEHQEGQLGLPEQQVLQLKQLEKQQGQPKHLEEEEGQLKHLVQQEGQLKHLVQQEGQLEQQERQVEHLEQQVGQLKHLEEQEGQLKHLEQQQGQLEVPEQQVGQPKNLEQEEKQLELPEQQEGQVKHLEKQEAQLELPEQQVGQPKHLEQQEKHLEHPEQQDGQLKHLEQQEGQLKDLEQQKGQLEQPVFAPAPGQVQDIQPALPTKGEVLLPVEHQQQKQEVQWPPKHK

PTMs - P07476 As Substrate

Site PTM Type Enzyme
S12 Phosphorylation
S16 Phosphorylation
T65 Phosphorylation
K314 Acetylation

Research Backgrounds

Function:

Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia.

PTMs:

Substrate of transglutaminase. Some glutamines and lysines are cross-linked to other involucrin molecules, to other proteins such as keratin, desmoplakin, periplakin and envoplakin, and to lipids like omega-hydroxyceramide.

Subcellular Location:

Cytoplasm.
Note: Constituent of the scaffolding of the cornified envelope.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Keratinocytes of epidermis and other stratified squamous epithelia.

Subunit Structure:

Directly or indirectly cross-linked to cornifelin (CNFN).

Family&Domains:

Belongs to the involucrin family.

References

1). Use of mouse primary epidermal organoids for USA300 infection modeling and drug screening. Research Square (PubMed: 36631452) [IF=9.0]

Application: IHC    Species: Mouse    Sample: mPEOs

Fig. 1: Characterization of mouse primary epidermal organoids (mPEOs).A Overview of mPEOs formed from single cells within 7 d (scale bars, 100 μm). B Increased number of mPEOs observed within 7 d in bright field (scale bars, 100 μm). C H&E staining of mPEOs cultured for 7 d (scale bars, 50 μm). D Ultrathin sections in TEM images of mPEOs (scale bars, 20 μm), SB stratum basale, SP stratum spinosum, GR stratum granulosum, KG keratohyalin granules, SC stratum corneum. E Immunofluorescence staining analysis of various skin-associated specific biomarkers in mPEOs (scale bars, 200 μm). F Immunohistochemical staining of paraffin-embedded mPEOs. Representative images of Loricrin, KRT10, Involucrin, KRT14 and Ki67 immunoreactivity in organoids (scale bars, 50 μm).

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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