Defensin beta-1 Antibody - #DF8530

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Product: Defensin beta-1 Antibody
Catalog: DF8530
Description: Rabbit polyclonal antibody to Defensin beta-1
Application: WB
Reactivity: Human
Mol.Wt.: 7 kDa; 7kD(Calculated).
Uniprot: P60022
RRID: AB_2841734

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Clonality:
Polyclonal
Specificity:
Defensin beta-1 Antibody detects endogenous levels of total Defensin beta-1.
RRID:
AB_2841734
Cite Format: Affinity Biosciences Cat# DF8530, RRID:AB_2841734.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BD 1; BD-1; BD1; beta 1; Beta defensin 1; Beta-defensin 1; DEFB 1; DEFB1; DEFB1_HUMAN; DEFB101; Defensin; Defensin beta 1; Defensin beta 1 preproprotein; HBD 1; hBD-1; HBD1; MGC51822;

Immunogens

Immunogen:
Uniprot:

P60022(DEFB1_HUMAN) >>Visit HPA database.

Gene(ID):
DEFB1(1672)
Expression:
P60022 DEFB1_HUMAN:

Blood plasma. Sperm. Highly expressed in the lower head and midpiece of sperm. Significantly reduced levels found in the sperms of asthenozoospermia and leukocytospermia patients (at protein level).

Sequence:
MRTSYLLLFTLCLLLSEMASGGNFLTGLGHRSDHYNCVSSGGQCLYSACPIFTKIQGTCYRGKAKCCK

Research Backgrounds

Function:

Has bactericidal activity. May act as a ligand for C-C chemokine receptor CCR6. Positively regulates the sperm motility and bactericidal activity in a CCR6-dependent manner. Binds to CCR6 and triggers Ca2+ mobilization in the sperm which is important for its motility.

Subcellular Location:

Secreted. Membrane.
Note: Associates with tumor cell membrane-derived microvesicles (PubMed:23938203).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Blood plasma. Sperm. Highly expressed in the lower head and midpiece of sperm. Significantly reduced levels found in the sperms of asthenozoospermia and leukocytospermia patients (at protein level).

Subunit Structure:

Monomer. Homodimer.

Family&Domains:

Belongs to the beta-defensin family.

References

1). Gut microbiota and butyrate contribute to nonalcoholic fatty liver disease in premenopause due to estrogen deficiency. PLoS One, 2022 (PubMed: 35108315) [IF=2.9]

Application: WB    Species: Mouse    Sample:

Fig 6. Decreased production of Reg3γ and β-defensins in IEC of OH mice. IEC were isolated from the small intestines of C, SH and OH mice, respectively. A: IEC expression of Reg3γ and β-defensins 1 and 3 in OH and the control mice were determined by qRT-PCR. B: Protein levels of Reg3γ and β-defensins 1 were determined by western blot. C: Relative density ratio of Reg3γ and β-defensins 1. IEC, intestinal epithelial cell; qRT-PCR, quantitative real-time polymerase chain reaction; C, normal diet (n = 8); SH, sham+HFD (n = 10); OH, OVX+HFD (n = 8); OVX, ovariectomy; HFD, high fat diet.

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