eEF1B2 Antibody - #DF8537

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Product: eEF1B2 Antibody
Catalog: DF8537
Description: Rabbit polyclonal antibody to eEF1B2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 24 kDa; 25kD(Calculated).
Uniprot: P24534
RRID: AB_2841741

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
eEF1B2 Antibody detects endogenous levels of total eEF1B2.
RRID:
AB_2841741
Cite Format: Affinity Biosciences Cat# DF8537, RRID:AB_2841741.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EEF1B; EEF1B1; EEF1B2; EF-1-beta; EF1B; EF1B_HUMAN; Elongation factor 1, beta-2-A; Elongation factor 1-beta; eukaryotic translation elongation factor 1 beta 1; eukaryotic translation elongation factor 1 beta 2;

Immunogens

Immunogen:
Uniprot:

P24534(EF1B_HUMAN) >>Visit HPA database.

Gene(ID):
EEF1B2(1933)
Sequence:
MGFGDLKSPAGLQVLNDYLADKSYIEGYVPSQADVAVFEAVSSPPPADLCHALRWYNHIKSYEKEKASLPGVKKALGKYGPADVEDTTGSGATDSKDDDDIDLFGSDDEEESEEAKRLREERLAQYESKKAKKPALVAKSSILLDVKPWDDETDMAKLEECVRSIQADGLVWGSSKLVPVGYGIKKLQIQCVVEDDKVGTDMLEEQITAFEDYVQSMDVAAFNKI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
67
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P24534 As Substrate

Site PTM Type Enzyme
K7 Acetylation
K7 Methylation
K7 Ubiquitination
S8 Phosphorylation
Y18 Phosphorylation
S42 Phosphorylation
S43 Phosphorylation
K60 Acetylation
K60 Ubiquitination
K78 Ubiquitination
Y79 Phosphorylation
T87 Phosphorylation
T88 Phosphorylation
S90 Phosphorylation
T93 Phosphorylation
S95 Phosphorylation
S106 Phosphorylation P19784 (CSNK2A2) , P68400 (CSNK2A1)
S112 Phosphorylation P19784 (CSNK2A2) , P68400 (CSNK2A1)
Y126 Phosphorylation
S128 Phosphorylation
K129 Acetylation
K129 Ubiquitination
K130 Ubiquitination
K132 Ubiquitination
K133 Ubiquitination
S141 Phosphorylation
K147 Acetylation
K147 Sumoylation
K147 Ubiquitination
S164 Phosphorylation
S174 Phosphorylation
K176 Acetylation
K176 Ubiquitination
Y182 Phosphorylation
K185 Acetylation
K185 Ubiquitination
K186 Ubiquitination
Y213 Phosphorylation

Research Backgrounds

Function:

EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP.

PTMs:

Phosphorylation affects the GDP/GTP exchange rate.

Subunit Structure:

EF-1 is composed of 4 subunits: alpha, beta, delta, and gamma.

Family&Domains:

Belongs to the EF-1-beta/EF-1-delta family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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