Nav1.6/SCN8A Antibody - #DF8577

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Product: Nav1.6/SCN8A Antibody
Catalog: DF8577
Description: Rabbit polyclonal antibody to Nav1.6/SCN8A
Application: WB IHC IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 225 kDa; 225kD(Calculated).
Uniprot: Q9UQD0
RRID: AB_2841781

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(94%), Bovine(94%), Horse(94%), Sheep(94%), Rabbit(88%), Dog(94%)
Clonality:
Polyclonal
Specificity:
Nav1.6/SCN8A Antibody detects endogenous levels of total Nav1.6/SCN8A.
RRID:
AB_2841781
Cite Format: Affinity Biosciences Cat# DF8577, RRID:AB_2841781.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CerIII; CIAT; EIEE13; hNa6/Scn8a voltage gated sodium channel; MED; Motor endplate disease; NaCh 6; NaCh6; Nav 1.6; Nbna1; peripheral nerve protein type 4; PN 4; PN4; SCN8A; SCN8A_HUMAN; Sodium channel protein type 8 alpha subunit; Sodium channel protein type 8 subunit alpha; Sodium channel protein type VIII alpha subunit; Sodium channel protein type VIII subunit alpha; Sodium channel voltage gated type VIII alpha; Sodium channel voltage gated type VIII alpha polypeptide; Sodium channel voltage gated type VIII alpha subunit; Voltage gated sodium channel subunit alpha Nav1.6; Voltage-gated sodium channel subunit alpha Nav1.6;

Immunogens

Immunogen:

A synthesized peptide derived from human Nav1.6/SCN8A, corresponding to a region within the internal amino acids.

Uniprot:

Q9UQD0(SCN8A_HUMAN) >>Visit HPA database.

Gene(ID):
SCN8A(6334)
Expression:
Q9UQD0 SCN8A_HUMAN:

Expressed in the hippocampus with increased expression in epileptic tissue compared to normal adjacent tissue (at protein level) (PubMed:28842554). Isoform 5: Expressed in non-neuronal tissues, such as monocytes/macrophages.

Sequence:
MAARLLAPPGPDSFKPFTPESLANIERRIAESKLKKPPKADGSHREDDEDSKPKPNSDLEAGKSLPFIYGDIPQGLVAVPLEDFDPYYLTQKTFVVLNRGKTLFRFSATPALYILSPFNLIRRIAIKILIHSVFSMIIMCTILTNCVFMTFSNPPDWSKNVEYTFTGIYTFESLVKIIARGFCIDGFTFLRDPWNWLDFSVIMMAYITEFVNLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVVWPINFNESYLENGTKGFDWEEYINNKTNFYTVPGMLEPLLCGNSSDAGQCPEGYQCMKAGRNPNYGYTSFDTFSWAFLALFRLMTQDYWENLYQLTLRAAGKTYMIFFVLVIFVGSFYLVNLILAVVAMAYEEQNQATLEEAEQKEAEFKAMLEQLKKQQEEAQAAAMATSAGTVSEDAIEEEGEEGGGSPRSSSEISKLSSKSAKERRNRRKKRKQKELSEGEEKGDPEKVFKSESEDGMRRKAFRLPDNRIGRKFSIMNQSLLSIPGSPFLSRHNSKSSIFSFRGPGRFRDPGSENEFADDEHSTVEESEGRRDSLFIPIRARERRSSYSGYSGYSQGSRSSRIFPSLRRSVKRNSTVDCNGVVSLIGGPGSHIGGRLLPEATTEVEIKKKGPGSLLVSMDQLASYGRKDRINSIMSVVTNTLVEELEESQRKCPPCWYKFANTFLIWECHPYWIKLKEIVNLIVMDPFVDLAITICIVLNTLFMAMEHHPMTPQFEHVLAVGNLVFTGIFTAEMFLKLIAMDPYYYFQEGWNIFDGFIVSLSLMELSLADVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKINQDCELPRWHMHDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLIVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDGEMNNLQISVIRIKKGVAWTKLKVHAFMQAHFKQREADEVKPLDELYEKKANCIANHTGADIHRNGDFQKNGNGTTSGIGSSVEKYIIDEDHMSFINNPNLTVRVPIAVGESDFENLNTEDVSSESDPEGSKDKLDDTSSSEGSTIDIKPEVEEVPVEQPEEYLDPDACFTEGCVQRFKCCQVNIEEGLGKSWWILRKTCFLIVEHNWFETFIIFMILLSSGALAFEDIYIEQRKTIRTILEYADKVFTYIFILEMLLKWTAYGFVKFFTNAWCWLDFLIVAVSLVSLIANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKYHYCFNETSEIRFEIEDVNNKTECEKLMEGNNTEIRWKNVKINFDNVGAGYLALLQVATFKGWMDIMYAAVDSRKPDEQPKYEDNIYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKIQGIVFDFVTQQAFDIVIMMLICLNMVTMMVETDTQSKQMENILYWINLVFVIFFTCECVLKMFALRHYYFTIGWNIFDFVVVILSIVGMFLADIIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIFSIFGMSNFAYVKHEAGIDDMFNFETFGNSMICLFQITTSAGWDGLLLPILNRPPDCSLDKEHPGSGFKGDCGNPSVGIFFFVSYIIISFLIVVNMYIAIILENFSVATEESADPLSEDDFETFYEIWEKFDPDATQFIEYCKLADFADALEHPLRVPKPNTIELIAMDLPMVSGDRIHCLDILFAFTKRVLGDSGELDILRQQMEERFVASNPSKVSYEPITTTLRRKQEEVSAVVLQRAYRGHLARRGFICKKTTSNKLENGGTHREKKESTPSTASLPSYDSVTKPEKEKQQRAEEGRRERAKRQKEVRESKC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
94
Horse
94
Bovine
94
Sheep
94
Dog
94
Rabbit
88
Chicken
75
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient.

In macrophages and melanoma cells, may participate in the control of podosome and invadopodia formation.

PTMs:

May be ubiquitinated by NEDD4L; which would promote its endocytosis.

Phosphorylation at Ser-1497 by PKC in a highly conserved cytoplasmic loop slows inactivation of the sodium channel and reduces peak sodium currents.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Cell projection>Axon.
Note: Mainly localizes to the axon initial segment.

Cytoplasmic vesicle.
Note: Some vesicles are localized adjacent to melanoma invadopodia and macrophage podosomes. Does not localize to the plasma membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in the hippocampus with increased expression in epileptic tissue compared to normal adjacent tissue (at protein level). Isoform 5: Expressed in non-neuronal tissues, such as monocytes/macrophages.

Family&Domains:

The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1, S2, S3, S5, S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.

Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.6/SCN8A subfamily.

References

1). Lidocaine inhibits influenza a virus replication by up-regulating IFNα4 via TBK1-IRF7 and JNK-AP1 signaling pathways. International Immunopharmacology, 2023 (PubMed: 36638664) [IF=4.8]
2). LncRNA01103/FGF13 axis promotes the progression of bone cancer pain by remodeling ac4C modification. Research Square, 2024

Application: WB    Species: Rat    Sample:

Figure 7. FGF13 participates in BCP by regulating sodium channels. (A) qPCR for monitoring FGF13-siRNA (siRNAFgf13) in RT4-D6P2T cells to validate transfection efficiency. Bars represent the means ± SEM of independent biological replicates (n = 3). Values of individual experiments are indicated as dots. P-values were calculated using the one-way ANOVA (**P < 0.01; ***P < 0.001). (B) Western blots for monitoring transfection efficiency of siRNAFgf13 in BCP rat SDH were validated. Bars represent the means ± SEM of independent biological replicates (n = 3). Values of individual experiments are indicated as dots. P-values were calculated using the one-way ANOVA (***P < 0.001). (C) Reducing Fgf13 expression attenuates mechanical nociceptive sensitization in BCP rats. Bars represent the means ± SEM of independent biological replicates (n = 8). P-values were calculated using the two-way repeated measures analysis (###P < 0.001). PWT: Paw withdrawal threshold. (D) Statistical plots of the number of buried strains in Sham, BCP+siRNANC and BCP+ siRNAFgf13 groups. Bars represent the means ± SEM of independent biological replicates (n = 8). Values of individual experiments are indicated as dots. P-values were calculated using the one-way ANOVA (**P < 0.01; ***P < 0.001). (E-G) Representative CatWalk gait, including footprint area and mean intensity (E), in Sham, BCP+ siRNANC and BCP+ siRNAFgf13 groups. Quantifications of mean intensity (LH/RH) (F) and print area (LH/RH) (G) in both groups of rats. Bars represent the means ± SEM of independent biological replicates (n = 8). Values of individual experiments are indicated as dots. P-values were calculated using the Student's t test (**P < 0.01; ***P < 0.001). LH, left hind paw; RH, right hind paw. (H-J) Representative traces of locomotor activity in the light-dark transition (LDT) (H) in Sham group, BCP+siRNANC group and BCP+siRNAFgf13 group rats. Quantifications of time spent (I) and distance (J) in the light chamber. Bars represent the means ± SEM of independent biological replicates (n = 8). Values of individual experiments are indicated as dots. P-values were calculated using the one-way ANOVA (**P < 0.01; ***P < 0.001). (K-L) Representative traces of Elevated plus- maze (EPM) (K) in Sham group, BCP+siRNANC group and BCP+siRNAFgf13 group rats. Quantifications of time spent (L) in the open arms. Bars represent the means ± SEM of independent biological replicates (n = 8). Values of individual experiments are indicated as dots. P-values were calculated using the one-way ANOVA (***P < 0.001). (M-P) Western blots for monitoring the effect of siRNAFgf13 on the expression of Nav1.1, Nav1.6, Nav1.7, Nav1.8 in the SDH of BCP rats. ß-Actin served as loading control. Bars represent the means ± SEM of independent biological replicates (n = 4). Values of individual experiments are indicated as dots. P-values were calculated using the one-way ANOVA (*P < 0.05; **P < 0.01).

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