OBCAM Antibody | Affinity Biosciences

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Product:	OBCAM Antibody
Catalog:	DF8584
Description:	Rabbit polyclonal antibody to OBCAM
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	38 kDa; 38kD(Calculated).
Uniprot:	Q14982
RRID:	AB_2841788

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(92%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%), Chicken(92%)

Clonality:

Polyclonal

Specificity:

OBCAM Antibody detects endogenous levels of total OBCAM.

RRID:

AB_2841788
Cite Format: Affinity Biosciences Cat# DF8584, RRID:AB_2841788.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

GM181; IgLON family member 1; IGLON1; OBCAM; OPCM; OPCM_HUMAN; OPCML; Opiate binding cell adhesion molecule; Opioid binding cell adhesion molecule; Opioid binding protein/cell adhesion molecule; Opioid binding protein/cell adhesion molecule-like; Opioid binding protein/cell adhesion molecule-like preprotein; Opioid-binding cell adhesion molecule; Opioid-binding protein/cell adhesion molecule;

Immunogens

Immunogen:

Uniprot:

Q14982(OPCM_HUMAN) >>Visit HPA database.

Gene(ID):

OPCML(4978)

Sequence:

Q14982 OPCM_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGVCGYLFLPWKCLVVVSLRLLFLVPTGVPVRSGDATFPKAMDNVTVRQGESATLRCTIDDRVTRVAWLNRSTILYAGNDKWSIDPRVIILVNTPTQYSIMIQNVDVYDEGPYTCSVQTDNHPKTSRVHLIVQVPPQIMNISSDITVNEGSSVTLLCLAIGRPEPTVTWRHLSVKEGQGFVSEDEYLEISDIKRDQSGEYECSALNDVAAPDVRKVKITVNYPPYISKAKNTGVSVGQKGILSCEASAVPMAEFQWFKEETRLATGLDGMRIENKGRMSTLTFFNVSEKDYGNYTCVATNKLGNTNASITLYGPGAVIDGVNSASRALACLWLSGTLLAHFFIKF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Dog

100

Rabbit

100

Pig

Bovine

Sheep

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14982 As Substrate

Q14982

Site	PTM Type	Enzyme	Source
K217	Ubiquitination		Uniprot
K228	Ubiquitination		Uniprot

Research Backgrounds

Q14982_OPCM_HUMAN

Function:

Binds opioids in the presence of acidic lipids; probably involved in cell contact.

Subcellular Location:

Cell membrane>Lipid-anchor.

Family&Domains:

Belongs to the immunoglobulin superfamily. IgLON family.

References

1). Comprehensive upstream and downstream regulatory analyses identify miR-675-3p as a potential prognostic biomarker in melanoma. Human Cell, 2021 (PubMed: 33400243) [IF=3.4]

Application: WB Species: Human Sample: melanoma A375 cells

Fig. 5 Identifcation of miR-675-3p target genes. a Pearson’s correlation analyses showed that miR-675-3p was signifcantly positively correlated with ERG1 and negatively correlated with ZBTB7 and IGF1R expression in the TCGA database. b Validation of transfection efciency. c Relative target gene mRNA expression. d Relative target gene protein expression. e OPCML was targeted by miR-675-3p in a dual luciferase reporter experiment

Restrictive clause

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For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

OBCAM Antibody - #DF8584