Pax-9 Antibody | Affinity Biosciences

WB
Cites

1/2

Pax-9 Antibody - Western blot analysis of extracts from MDA-MB-231 cells, using Pax-9 Antibody.

Product:	Pax-9 Antibody
Catalog:	DF8589
Description:	Rabbit polyclonal antibody to Pax-9
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	36 kDa,55kDa; 36kD(Calculated).
Uniprot:	P55771
RRID:	AB_2841793

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(91%), Xenopus(92%)

Clonality:

Polyclonal

Specificity:

Pax-9 Antibody detects endogenous levels of total Pax-9.

RRID:

AB_2841793
Cite Format: Affinity Biosciences Cat# DF8589, RRID:AB_2841793.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Paired box 9; Paired box gene 9; Paired box homeotic gene 9; Paired box protein 9; Paired box protein Pax 9; Paired box protein Pax-9; Paired box protein Pax9; Paired domain gene 9; PAX 9; PAX9; PAX9_HUMAN; STHAG3;

Immunogens

Immunogen:

Uniprot:

P55771(PAX9_HUMAN) >>Visit HPA database.

Gene(ID):

PAX9(5083)

Sequence:

P55771 PAX9_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEPAFGEVNQLGGVFVNGRPLPNAIRLRIVELAQLGIRPCDISRQLRVSHGCVSKILARYNETGSILPGAIGGSKPRVTTPTVVKHIRTYKQRDPGIFAWEIRDRLLADGVCDKYNVPSVSSISRILRNKIGNLAQQGHYDSYKQHQPTPQPALPYNHIYSYPSPITAAAAKVPTPPGVPAIPGSVAMPRTWPSSHSVTDILGIRSITDQVSDSSPYHSPKVEEWSSLGRNNFPAAAPHAVNGLEKGALEQEAKYGQAPNGLPAVGSFVSASSMAPYPTPAQVSPYMTYSAAPSGYVAGHGWQHAGGTSLSPHNCDIPASLAFKGMQAAREGSHSVTASAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Chicken

Zebrafish

Horse

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P55771 As Substrate

P55771

Site	PTM Type	Source
K114	Ubiquitination	Uniprot
K144	Ubiquitination	Uniprot
T175	Phosphorylation	Uniprot
S197	Phosphorylation	Uniprot
S219	Phosphorylation	Uniprot

Research Backgrounds

P55771_PAX9_HUMAN

Function:

Transcription factor required for normal development of thymus, parathyroid glands, ultimobranchial bodies, teeth, skeletal elements of skull and larynx as well as distal limbs.

Subcellular Location:

Nucleus.

Subunit Structure:

Interacts with KDM5B.

References

1). Paired box 9 regulates VSMC phenotypic transformation, proliferation, and migration via sonic hedgehog. LIFE SCIENCES, 2020 (PubMed: 32634424) [IF=5.2]

Application: WB Species: mouse Sample: Aortic

Figure 1. |Pax9 is increased in the mouse model of AS. (E) Immunofluorescence staining. Aortic tissue sections were stained with an anti-Pax9 antibody (red) and DAPI for the nuclei (blue). (F) Western blot detection and (G) quantification of the relative protein expression of Pax9 in the control group and HFD group were performed. GAPDH was used as a loading control. ∗∗∗p < 0.001; ∗∗p < 0.01; scale bar: 100μm. Data are expressed as the means± SD (n = 12).

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

Pax-9 Antibody - #DF8589