Podocin Antibody | Affinity Biosciences

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Podocin Antibody - Western blot analysis of extracts from Mouse testis, using Podocin Antibody at 1/1000 dilution.

Product:	Podocin Antibody
Catalog:	DF8593
Description:	Rabbit polyclonal antibody to Podocin
Application:	WB
Reactivity:	Human
Prediction:	Mouse, Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	42 kDa; 42kD(Calculated).
Uniprot:	Q9NP85
RRID:	AB_2841797

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Mouse(%), Pig(93%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(93%), Dog(86%)

Clonality:

Polyclonal

Specificity:

Podocin Antibody detects endogenous levels of total Podocin.

RRID:

AB_2841797
Cite Format: Affinity Biosciences Cat# DF8593, RRID:AB_2841797.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Nephrosis 2, idiopathic, steroid resistant (podocin); nephrosis 2, idiopathic, steroid resistant; NPHS2; NPHS2 gene; PDCN; PODO_HUMAN; Podocin; SRN1;

Immunogens

Immunogen:

Uniprot:

Q9NP85(PODO_HUMAN) >>Visit HPA database.

Gene(ID):

NPHS2(7827)

Expression:

Q9NP85 PODO_HUMAN:

Almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli.

Sequence:

Q9NP85 PODO_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MERRARSSSRESRGRGGRTPHKENKRAKAERSGGGRGRQEAGPEPSGSGRAGTPGEPRAPAATVVDVDEVRGSGEEGTEVVALLESERPEEGTKSSGLGACEWLLVLISLLFIIMTFPFSIWFCVKVVQEYERVIIFRLGHLLPGRAKGPGLFFFLPCLDTYHKVDLRLQTLEIPFHEIVTKDMFIMEIDAICYYRMENASLLLSSLAHVSKAVQFLVQTTMKRLLAHRSLTEILLERKSIAQDAKVALDSVTCIWGIKVERIEIKDVRLPAGLQHSLAVEAEAQRQAKVRMIAAEAEKAASESLRMAAEILSGTPAAVQLRYLHTLQSLSTEKPSTVVLPLPFDLLNCLSSPSNRTQGSLPFPSPSKPVEPLNPKKKDSPML

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Pig

Rabbit

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NP85 As Substrate

Q9NP85

Site	PTM Type	Source
Y194	Phosphorylation	Uniprot
Y195	Phosphorylation	Uniprot
S211	Phosphorylation	Uniprot
S277	Phosphorylation	Uniprot
S302	Phosphorylation	Uniprot
S313	Phosphorylation	Uniprot

Research Backgrounds

Q9NP85_PODO_HUMAN

Function:

Plays a role in the regulation of glomerular permeability, acting probably as a linker between the plasma membrane and the cytoskeleton.

PTMs:

Isoform 2 is glycosylated.

Subcellular Location:

Cell membrane>Peripheral membrane protein.

Endoplasmic reticulum.

Tissue Specificity:

Almost exclusively expressed in the podocytes of fetal and mature kidney glomeruli.

Subunit Structure:

Interacts with nephrin/NPHS1 and KIRREL1. Interacts directly with CD2AP. Interacts with DDN (By similarity).

Family&Domains:

Belongs to the band 7/mec-2 family.

References

1). Targeted inhibition of CX3CL1 limits podocytes ferroptosis to ameliorate cisplatin-induced acute kidney injury. Molecular medicine (Cambridge, Mass.), 2023 (PubMed: 37875838) [IF=6.0]

Application: WB Species: Mouse Sample: kidney tissues

Fig. 1 CX3CL1 deficiency alleviated kidney function damage in cisplatin-induced model mice. WT mice and CX3CL1-KO mice received cisplatin injections at 48 h. A Examination of the kidney’s histology using representative images of H&E and PAS staining. B The serum levels of BUN and Scr were evaluated in all mice groups. C Western blotting was conducted to determine the relative expression patterns of podocin, CX3CL1, nephrin, and WT1. D Representative images of podocyte ultrastructure in kidney tissues following cisplatin injection, as shown in TEM. Scale bar = 500 nm. E Representative micrographs of CX3CL1 and nephrin staining in kidney tissues. Scale bar = 10 μm. (WT, wild type; KO, knockdown; H&E, hematoxylin and eosin; PAS, periodic acid-Schiff; BUN, blood urea nitrogen; Scr, serum creatinine; WT1, Wilms tumor protein; TEM, transmission electron microscopy; p value was calculated by one-way analysis of variance and Tukey’s test.

2). Phloretin ameliorates diabetic nephropathy by inhibiting nephrin and podocin reduction through a non-hypoglycemic effect. Food & Function, 2022 (PubMed: 35622066) [IF=5.1]

3). The Yi-Qi-Bu-Shen recipe attenuates high glucose-induced podocyte injury via the inhibition of IKK-IκBα-NFκB and ERK/P38 MAPK signaling . International Journal of Clinical and Experimental Medicine, 2022 [IF=0.2]

Application: WB Species: Human Sample: podocytes

Figure 2. Effect of YB on HG-induced expression of nephrin, podocin and CD2AP in podocytes. A-C. Podocytes were treated with HG and YB for 24 hours and then mRNA levels of nephrin, podocin and CD2AP were determined by qRT-PCR. (*P

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Podocin Antibody - #DF8593