RBMS3 Antibody - #DF8599

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Product: RBMS3 Antibody
Catalog: DF8599
Description: Rabbit polyclonal antibody to RBMS3
Application: WB IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 47 kDa; 48kD(Calculated).
Uniprot: Q6XE24
RRID: AB_2841803

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(92%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
RBMS3 Antibody detects endogenous levels of total RBMS3.
RRID:
AB_2841803
Cite Format: Affinity Biosciences Cat# DF8599, RRID:AB_2841803.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

RNA binding motif, single stranded interacting protein 3; RNA binding motif, single stranded interacting protein; RNA binding motif, single stranded-interacting 3; RNA binding protein;

Immunogens

Immunogen:

A synthesized peptide derived from human RBMS3, corresponding to a region within the internal amino acids.

Uniprot:

Q6XE24(RBMS3_HUMAN) >>Visit HPA database.

Gene(ID):
RBMS3(27303)
Expression:
Q6XE24 RBMS3_HUMAN:

Expressed in fetal brain, fetal lung, fetal liver, heart, brain, placenta, lung, liver, muscle, kidney and pancreas.

Sequence:
MGKRLDQPQMYPQYTYYYPHYLQTKQSYAPAPHPMAPPSPSTNSSSNNSSNNSSGEQLSKTNLYIRGLPPGTTDQDLIKLCQPYGKIVSTKAILDKNTNQCKGYGFVDFDSPAAAQKAVASLKANGVQAQMAKQQEQDPTNLYISNLPISMDEQELENMLKPFGHVISTRILRDANGVSRGVGFARMESTEKCEVVIQHFNGKYLKTPPGIPAPSEPLLCKFADGGQKKRQNQSKYTQNGRPWPREGEAGMALTYDPTAAIQNGFYSSPYSIATNRMIPQTSITPFIAASPVSTYQVQSTSWMPHPPYVMQPTGAVITPTMDHPMSMQPANMMGPLTQQMNHLSLGTTGTIQSQDRIMILHQLLCQYMTAAAPMQGTYIPQYTPVPPTAVSIEGVVADTSPQTVAPSSQDTSGQQQQIAVDTSNEHAPAYSYQQSKP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Zebrafish
92
Horse
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Binds poly(A) and poly(U) oligoribonucleotides.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in fetal brain, fetal lung, fetal liver, heart, brain, placenta, lung, liver, muscle, kidney and pancreas.

References

1). Tumor suppressor function of RBMS3 overexpression in EOC associated with immune cell infiltration. Heliyon, 2024 (PubMed: 38726149) [IF=4.0]

Application: WB    Species: human    Sample:

Fig. 1 The poor prognosis of OC patients is linked to down-regulated RBMS3 expression in OC tissues. (A) RBMS3 mRNA expression levels from the GEPIA database in both normal tissue and OC. Grey (n = 88) normal samples and red (n = 426) tumor samples. (B, C)Data on OS and PFS for patients with OC were collected with the assistant of the Kaplan-Meier plotter (https://kmplot.com/analysis/). (D) qRT-PCR was used to measure the mRNA levels of RBMS3 in 29 instances of EOC and 23 cases of normal ovarian tissue. (E, F) Western blot analysis reveals the RBMS3 protein expression level in eight pairs of EOC tissues. (G) Typical IHC staining pictures of RBMS3 in normal tissue (Normal) and OC (Tumor). The scale bars are 50 μm and 20 μm respectively. (H, I) Kaplan-Meier study of the effects of 60 EOC patients' RBMS3 expression on OS and PFS. *P < 0.05, **P < 0.01, ***P < 0.001 and****P < 0.0001.The uncropped versions of Fig. 1E is in Supplementary Figs. S1 and S2.

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