TRPV4 Antibody - #DF8624

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Product: TRPV4 Antibody
Catalog: DF8624
Description: Rabbit polyclonal antibody to TRPV4
Application: WB IHC IF/ICC
Cited expt.: WB, IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Sheep, Rabbit
Mol.Wt.: 98 kDa; 98kD(Calculated).
Uniprot: Q9HBA0
RRID: AB_2841828

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(82%), Bovine(100%), Sheep(100%), Rabbit(100%)
Clonality:
Polyclonal
Specificity:
TRPV4 Antibody detects endogenous levels of total TRPV4.
RRID:
AB_2841828
Cite Format: Affinity Biosciences Cat# DF8624, RRID:AB_2841828.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BCYM3; CMT2C; HMSN2C; osm 9 like TRP channel 4; Osm-9-like TRP channel 4; OSM9 like transient receptor potential channel 4; Osmosensitive transient receptor potential channel 4; OTRPC 4; OTRPC4; SMAL; SPSMA; SSQTL1; Transient receptor potential cation channel subfamily V member 4; Transient receptor potential protein 12; TRP 12; TRP12; TRPV 4; TrpV4; TRPV4_HUMAN; Vanilloid receptor like channel 2; Vanilloid receptor like protein 2; Vanilloid receptor related osmotically activated channel; Vanilloid receptor-like channel 2; Vanilloid receptor-like protein 2; Vanilloid receptor-related osmotically-activated channel; VR 4; VR OAC; VR-OAC; VR4; VRL 2; VRL-2; VRL2; VROAC;

Immunogens

Immunogen:

A synthesized peptide derived from human TRPV4, corresponding to a region within C-terminal amino acids.

Uniprot:

Q9HBA0(TRPV4_HUMAN) >>Visit HPA database.

Gene(ID):
TRPV4(59341)
Expression:
Q9HBA0 TRPV4_HUMAN:

Found in the synoviocytes from patients with (RA) and without (CTR) rheumatoid arthritis (at protein level).

Sequence:
MADSSEGPRAGPGEVAELPGDESGTPGGEAFPLSSLANLFEGEDGSLSPSPADASRPAGPGDGRPNLRMKFQGAFRKGVPNPIDLLESTLYESSVVPGPKKAPMDSLFDYGTYRHHSSDNKRWRKKIIEKQPQSPKAPAPQPPPILKVFNRPILFDIVSRGSTADLDGLLPFLLTHKKRLTDEEFREPSTGKTCLPKALLNLSNGRNDTIPVLLDIAERTGNMREFINSPFRDIYYRGQTALHIAIERRCKHYVELLVAQGADVHAQARGRFFQPKDEGGYFYFGELPLSLAACTNQPHIVNYLTENPHKKADMRRQDSRGNTVLHALVAIADNTRENTKFVTKMYDLLLLKCARLFPDSNLEAVLNNDGLSPLMMAAKTGKIGIFQHIIRREVTDEDTRHLSRKFKDWAYGPVYSSLYDLSSLDTCGEEASVLEILVYNSKIENRHEMLAVEPINELLRDKWRKFGAVSFYINVVSYLCAMVIFTLTAYYQPLEGTPPYPYRTTVDYLRLAGEVITLFTGVLFFFTNIKDLFMKKCPGVNSLFIDGSFQLLYFIYSVLVIVSAALYLAGIEAYLAVMVFALVLGWMNALYFTRGLKLTGTYSIMIQKILFKDLFRFLLVYLLFMIGYASALVSLLNPCANMKVCNEDQTNCTVPTYPSCRDSETFSTFLLDLFKLTIGMGDLEMLSSTKYPVVFIILLVTYIILTFVLLLNMLIALMGETVGQVSKESKHIWKLQWATTILDIERSFPVFLRKAFRSGEMVTVGKSSDGTPDRRWCFRVDEVNWSHWNQNLGIINEDPGKNETYQYYGFSHTVGRLRRDRWSSVVPRVVELNKNSNPDEVVVPLDSMGNPRCDGHQQGYPRKWRTDDAPL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Rabbit
100
Zebrafish
82
Xenopus
73
Chicken
67
Horse
0
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by heat, low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers (By similarity). Acts as a regulator of intracellular Ca(2+) in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8. Together with PKD2, forms mechano- and thermosensitive channels in cilium. Negatively regulates expression of PPARGC1A, UCP1, oxidative metabolism and respiration in adipocytes (By similarity). Regulates expression of chemokines and cytokines related to proinflammatory pathway in adipocytes (By similarity). Together with AQP5, controls regulatory volume decrease in salivary epithelial cells (By similarity). Required for normal development and maintenance of bone and cartilage.

Non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by phorbol esters. Has the same channel activity as isoform 1, and is activated by the same stimuli.

Lacks channel activity, due to impaired oligomerization and intracellular retention.

Lacks channel activity, due to impaired oligomerization and intracellular retention.

Lacks channel activity, due to impaired oligomerization and intracellular retention.

PTMs:

N-glycosylated.

Subcellular Location:

Apical cell membrane>Multi-pass membrane protein. Cell junction>Adherens junction. Cell projection>Cilium.
Note: Assembly of the putative homotetramer occurs primarily in the endoplasmic reticulum.

Cell membrane.

Cell membrane.

Endoplasmic reticulum.

Endoplasmic reticulum.

Endoplasmic reticulum.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Found in the synoviocytes from patients with (RA) and without (CTR) rheumatoid arthritis (at protein level).

Family&Domains:

The ANK repeat region mediates interaction with Ca(2+)-calmodulin and ATP binding (By similarity). The ANK repeat region mediates interaction with phosphatidylinositol-4,5-bisphosphate and related phosphatidylinositides (PubMed:25256292).

Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV4 sub-subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Organismal Systems > Sensory system > Inflammatory mediator regulation of TRP channels.   (View pathway)

References

1). Near-Infrared Light-Controlled Dynamic Hydrogel for Modulating Mechanosensitive Ion Channels in 3-Dimensional Environment. Biomaterials research, 2025 (PubMed: 40207256) [IF=11.3]
2). Shexiang Baoxin Pill treats acute myocardial infarction by promoting angiogenesis via GDF15-TRPV4 signaling. Biomedicine & Pharmacotherapy, 2023 [IF=6.9]

Application: WB    Species: Human    Sample:

Fig. 6. GDF15 knockdown was associated with lowered transient receptor potential ion channel protein V4 (TRPV4) expression and intracellular calcium signaling. Representative A) Western blot image, as well as quantification of B) GDF15 and C) TRPV4 protein expression levels among the 4 HUVEC groups. D) Intracellular calcium concentration, indicated by Cal-590 AM loading, among the 4 treatment groups. n = 3/group for B) and C). *P 
3). Low-Intensity Extracorporeal Shock Wave Therapy Ameliorates Detrusor Hyperactivity with Impaired Contractility via Transient Potential Vanilloid Channels: A Rat Model for Ovarian Hormone Deficiency. International journal of molecular sciences, 2024 (PubMed: 38732143) [IF=5.6]

Application: WB    Species: Rat    Sample:

Figure 4 LiESWT increased neuronal regeneration, synaptic transmission, and receptor response. The expressions of neuronal endogenous markers (NF, NeuN, and GFAP) and muscarinic receptor (M2 and M3), and purinergic receptor (P2X7) markers were assessed by immunostaining (A–H) and Western blots (I,J). Nuclear DNA was labeled with DAPI (blue). (A–D) The M2 immunostaining was markedly expressed in the UL and SL of the (A) sham group. On the contrary, there was less M2 staining expression in the thinner and defective urothelial mucosa in the UL (yellow arrows) of the (B) OVX group, but the immunostainings in the (C) OVX + SW4 group and the (D) OVX + SW8 group were enhanced. (E–H) Double-labeled analysis of M2 (red, upper panels) and NF (green, lower panels) was distributed in the ML of the (E) sham group. However, the double staining of the (G) OVX + SW4 group and the (H) OVX + SW8 group were widely expressed compared with the (F) OVX group. (I,J) Quantifications of the percentage of neurogenesis-related markers, muscarinic receptors, and purinergic receptors were evaluated by Western blotting. The expressions obviously decreased in the OVX group compared with the sham group. However, the expressions significantly increased in the OVX + SW4 group and OVX + SW8 group compared with the OVX group. Therefore, the LiESWT promoted bladder synaptic transmission, receptor response, and neurogenesis to ameliorate the bladder detrusor contractile response. Nuclear DNA was labeled with DAPI (blue). LiESWT, low-intensity extracorporeal shock wave therapy; OVX + SW4, OHD status for 12 months, followed by once weekly LiESWT for 4 weeks; OVX + SW8, OHD status for 12 months, followed by twice weekly LiESWT for 4 weeks; DAPI, 4′,6-diamidino-2-phenylindole; NF, neurofilament; NeuN, neuronal nuclear antigen and neuron; GFAP, glial fibrillary acidic protein; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; UL, urothelial layer; SL, suburothelial layer. Data are expressed as the means ± SD for n = 8. * p < 0.05 and ** p < 0.01 versus the sham group; ## p < 0.01 versus the OVX group; †† p < 0.01 versus the OVX + SW4 group.

Application: IF/ICC    Species: Rat    Sample:

Figure 4 LiESWT increased neuronal regeneration, synaptic transmission, and receptor response. The expressions of neuronal endogenous markers (NF, NeuN, and GFAP) and muscarinic receptor (M2 and M3), and purinergic receptor (P2X7) markers were assessed by immunostaining (A–H) and Western blots (I,J). Nuclear DNA was labeled with DAPI (blue). (A–D) The M2 immunostaining was markedly expressed in the UL and SL of the (A) sham group. On the contrary, there was less M2 staining expression in the thinner and defective urothelial mucosa in the UL (yellow arrows) of the (B) OVX group, but the immunostainings in the (C) OVX + SW4 group and the (D) OVX + SW8 group were enhanced. (E–H) Double-labeled analysis of M2 (red, upper panels) and NF (green, lower panels) was distributed in the ML of the (E) sham group. However, the double staining of the (G) OVX + SW4 group and the (H) OVX + SW8 group were widely expressed compared with the (F) OVX group. (I,J) Quantifications of the percentage of neurogenesis-related markers, muscarinic receptors, and purinergic receptors were evaluated by Western blotting. The expressions obviously decreased in the OVX group compared with the sham group. However, the expressions significantly increased in the OVX + SW4 group and OVX + SW8 group compared with the OVX group. Therefore, the LiESWT promoted bladder synaptic transmission, receptor response, and neurogenesis to ameliorate the bladder detrusor contractile response. Nuclear DNA was labeled with DAPI (blue). LiESWT, low-intensity extracorporeal shock wave therapy; OVX + SW4, OHD status for 12 months, followed by once weekly LiESWT for 4 weeks; OVX + SW8, OHD status for 12 months, followed by twice weekly LiESWT for 4 weeks; DAPI, 4′,6-diamidino-2-phenylindole; NF, neurofilament; NeuN, neuronal nuclear antigen and neuron; GFAP, glial fibrillary acidic protein; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; UL, urothelial layer; SL, suburothelial layer. Data are expressed as the means ± SD for n = 8. * p < 0.05 and ** p < 0.01 versus the sham group; ## p < 0.01 versus the OVX group; †† p < 0.01 versus the OVX + SW4 group.
4). Investigating the Impact of Estrogen Levels on Voiding Characteristics, Bladder Structure, and Related Proteins in a Mouse Model of Menopause-Induced Lower Urinary Tract Symptoms. Biomolecules, 2024 (PubMed: 39334811) [IF=5.5]
5). Excessive mechanical stress induces chondrocyte apoptosis through TRPV4 in an anterior cruciate ligament-transected rat osteoarthritis model. LIFE SCIENCES, 2019 (PubMed: 31055086) [IF=5.2]

Application: WB    Species: rat    Sample: articular cartilage

Fig. 2. |TRPV4 inhibitor inhibited the high expression of calmodulin and cleaved caspase-8. (A) TRPV4, calmodulin,cleaved caspase-8 protein expressions were detected by western blotting
6). Voluntary wheel exercise improves glymphatic clearance and ameliorates colitis-associated cognitive impairment in aged mice by inhibiting TRPV4-induced astrocytic calcium activity. Experimental neurology, 2024 (PubMed: 38580155) [IF=4.6]
7). Activation of TRPV4 induces intraneuronal tau hyperphosphorylation via cholesterol accumulation. Experimental neurology, 2023 (PubMed: 36990137) [IF=4.6]
8). Simulated microgravity-induced oxidative stress and loss of osteogenic potential of osteoblasts can be prevented by protection of primary cilia. Journal of cellular physiology, 2023 (PubMed: 37796139) [IF=4.5]
9). Investigation of local stimulation effects of embedding PGLA at Zusanli (ST36) acupoint in rats based on TRPV2 and TRPV4 ion channels. Frontiers in neuroscience, 2024 (PubMed: 39445077) [IF=4.3]
10). Small extracellular vesicles derived from miRNA-486 overexpressed dental pulp stem cells mitigate high altitude pulmonary edema through PTEN/PI3K/AKT/eNOS pathway. Heliyon, 2025 (PubMed: 39906863) [IF=4.0]
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