ZFP598 Antibody - #DF8631

(3)  
Product: ZFP598 Antibody
Catalog: DF8631
Description: Rabbit polyclonal antibody to ZFP598
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 98 kDa; 99kD(Calculated).
Uniprot: Q86UK7
RRID: AB_2841835

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
ZFP598 Antibody detects endogenous levels of total ZFP598.
RRID:
AB_2841835
Cite Format: Affinity Biosciences Cat# DF8631, RRID:AB_2841835.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Zinc finger protein 598; ZN598_HUMAN; Znf598;

Immunogens

Immunogen:
Uniprot:

Q86UK7(ZN598_HUMAN) >>Visit HPA database.

Gene(ID):
ZNF598(90850)
Sequence:
MAAAGGAEGRRAALEAAAAAAPERGGGSCVLCCGDLEATALGRCDHPVCYRCSTKMRVLCEQRYCAVCREELRQVVFGKKLPAFATIPIHQLQHEKKYDIYFADGKVYALYRQLLQHECPRCPELPPFSLFGDLEQHMRRQHELFCCRLCLQHLQIFTYERKWYSRKDLARHRMQGDPDDTSHRGHPLCKFCDERYLDNDELLKHLRRDHYFCHFCDSDGAQDYYSDYAYLREHFREKHFLCEEGRCSTEQFTHAFRTEIDLKAHRTACHSRSRAEARQNRHIDLQFSYAPRHSRRNEGVVGGEDYEEVDRYSRQGRVARAGTRGAQQSRRGSWRYKREEEDREVAAAVRASVAAQQQEEARRSEDQEEGGRPKKEEAAARGPEDPRGPRRSPRTQGEGPGPKETSTNGPVSQEAFSVTGPAAPGCVGVPGALPPPSPKLKDEDFPSLSASTSSSCSTAATPGPVGLALPYAIPARGRSAFQEEDFPALVSSVPKPGTAPTSLVSAWNSSSSSKKVAQPPLSAQATGSGQPTRKAGKGSRGGRKGGPPFTQEEEEDGGPALQELLSTRPTGSVSSTLGLASIQPSKVGKKKKVGSEKPGTTLPQPPPATCPPGALQAPEAPASRAEGPVAVVVNGHTEGPAPARSAPKEPPGLPRPLGSFPCPTPQEDFPALGGPCPPRMPPPPGFSAVVLLKGTPPPPPPGLVPPISKPPPGFSGLLPSPHPACVPSPATTTTTKAPRLLPAPRAYLVPENFRERNLQLIQSIRDFLQSDEARFSEFKSHSGEFRQGLISAAQYYKSCRDLLGENFQKVFNELLVLLPDTAKQQELLSAHTDFCNREKPLSTKSKKNKKSAWQATTQQAGLDCRVCPTCQQVLAHGDASSHQALHAARDDDFPSLQAIARIIT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q86UK7 As Substrate

Site PTM Type Enzyme
K80 Ubiquitination
T86 Phosphorylation
K96 Ubiquitination
K97 Ubiquitination
Y108 Phosphorylation
Y111 Phosphorylation
K190 Ubiquitination
K204 Ubiquitination
Y211 Phosphorylation
Y224 Phosphorylation
Y230 Phosphorylation
K238 Ubiquitination
K263 Methylation
K263 Ubiquitination
S288 Phosphorylation
Y289 Phosphorylation
R292 Methylation
Y306 Phosphorylation
Y312 Phosphorylation
S313 Phosphorylation
R320 Methylation
R324 Methylation
Y336 Phosphorylation
S392 Phosphorylation
T395 Phosphorylation
S437 Phosphorylation
T461 Phosphorylation
Y471 Phosphorylation
S479 Phosphorylation
S502 Phosphorylation
S528 Phosphorylation
S539 Phosphorylation
T550 Phosphorylation
R655 Methylation
R679 Methylation
T695 Phosphorylation
S720 Phosphorylation
S728 Phosphorylation
K779 Methylation
K839 Acetylation
K844 Acetylation
K847 Acetylation
K850 Ubiquitination
C864 S-Nitrosylation
S895 Phosphorylation

Research Backgrounds

Function:

E3 ubiquitin-protein ligase that plays a key role in the ribosome quality control (RQC), a pathway that takes place when a ribosome has stalled during translation. Required for ribosomes to terminally stall during translation of poly(A) sequences by mediating monoubiquitination of 40S ribosomal protein RPS10/eS10, RPS20/uS10 and RPS3/uS3. Stalling precludes synthesis of a long poly-lysine tail and initiates the RQC pathway to degrade the potentially detrimental aberrant nascent polypeptide. Also acts as a component of the 4EHP-GYF2 complex, a multiprotein complex that acts as a repressor of translation initiation.

Subunit Structure:

Component of the 4EHP-GYF2 complex, at least composed of EIF4E2, GIGYF2 and ZNF598.

Family&Domains:

Belongs to the ZNF598 family.

Restrictive clause

 

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