APC8 Antibody - #DF8637

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Product: APC8 Antibody
Catalog: DF8637
Description: Rabbit polyclonal antibody to APC8
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 68 kDa; 69kD(Calculated).
Uniprot: Q9UJX2
RRID: AB_2841841

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
APC8 Antibody detects endogenous levels of total APC8.
RRID:
AB_2841841
Cite Format: Affinity Biosciences Cat# DF8637, RRID:AB_2841841.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ANAPC8; Anaphase promoting complex subunit 8; Anaphase-promoting complex subunit 8; Apc 8; APC8; Cdc 23; CDC23; CDC23_HUMAN; cell division cycle 23; Cell division cycle 23 homolog; Cell division cycle protein 23; Cell division cycle protein 23 homolog; Cut23; Cyclosome subunit 8;

Immunogens

Immunogen:
Uniprot:

Q9UJX2(CDC23_HUMAN) >>Visit HPA database.

Gene(ID):
CDC23(8697)
Sequence:
MAASTSMVPVAVTAAVAPVLSINSDFSDLREIKKQLLLIAGLTRERGLLHSSKWSAELAFSLPALPLAELQPPPPITEEDAQDMDAYTLAKAYFDVKEYDRAAHFLHGCNSKKAYFLYMYSRYLSGEKKKDDETVDSLGPLEKGQVKNEALRELRVELSKKHQARELDGFGLYLYGVVLRKLDLVKEAIDVFVEATHVLPLHWGAWLELCNLITDKEMLKFLSLPDTWMKEFFLAHIYTELQLIEEALQKYQNLIDVGFSKSSYIVSQIAVAYHNIRDIDKALSIFNELRKQDPYRIENMDTFSNLLYVRSMKSELSYLAHNLCEIDKYRVETCCVIGNYYSLRSQHEKAALYFQRALKLNPRYLGAWTLMGHEYMEMKNTSAAIQAYRHAIEVNKRDYRAWYGLGQTYEILKMPFYCLYYYRRAHQLRPNDSRMLVALGECYEKLNQLVEAKKCYWRAYAVGDVEKMALVKLAKLHEQLTESEQAAQCYIKYIQDIYSCGEIVEHLEESTAFRYLAQYYFKCKLWDEASTCAQKCCAFNDTREEGKALLRQILQLRNQGETPTTEVPAPFFLPASLSANNTPTRRVSPLNLSSVTP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
88
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UJX2 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S21 Phosphorylation
K34 Ubiquitination
T43 Phosphorylation
K97 Ubiquitination
K112 Ubiquitination
Y115 Phosphorylation
Y120 Phosphorylation
S121 Phosphorylation
K143 Ubiquitination
K147 Sumoylation
K147 Ubiquitination
S223 Phosphorylation
Y273 Phosphorylation
K281 Ubiquitination
S284 Phosphorylation
K291 Ubiquitination
K313 Ubiquitination
K328 Ubiquitination
Y329 Phosphorylation
Y340 Phosphorylation
Y341 Phosphorylation
K349 Ubiquitination
Y353 Phosphorylation
K359 Ubiquitination
K396 Ubiquitination
K445 Ubiquitination
K453 Ubiquitination
Y460 Phosphorylation
K467 Acetylation
K467 Ubiquitination
K472 Ubiquitination
K475 Ubiquitination
K522 Methylation
K524 Ubiquitination
S530 Phosphorylation
K535 Ubiquitination
T542 Phosphorylation
K547 Ubiquitination
T562 Phosphorylation P53350 (PLK1) , P06493 (CDK1)
T564 Phosphorylation
T565 Phosphorylation P53350 (PLK1) , P06493 (CDK1)
S576 Phosphorylation
S578 Phosphorylation
T582 Phosphorylation
T584 Phosphorylation
S588 Phosphorylation
S593 Phosphorylation
S594 Phosphorylation
T596 Phosphorylation

Research Backgrounds

Function:

Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.

PTMs:

Phosphorylated. Phosphorylation on Thr-562 occurs specifically during mitosis.

Subunit Structure:

The mammalian APC/C is composed at least of 14 distinct subunits ANAPC1, ANAPC2, CDC27/APC3, ANAPC4, ANAPC5, CDC16/APC6, ANAPC7, CDC23/APC8, ANAPC10, ANAPC11, CDC26/APC12, ANAPC13, ANAPC15 and ANAPC16 that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa; APC/C interacts with FZR1 and FBXO5.

Family&Domains:

Belongs to the APC8/CDC23 family.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > Oocyte meiosis.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

Restrictive clause

 

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