DIL-2 Antibody - #DF8650

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Product: DIL-2 Antibody
Catalog: DF8650
Description: Rabbit polyclonal antibody to DIL-2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
Mol.Wt.: 85 kDa; 86kD(Calculated).
Uniprot: Q9ULW0
RRID: AB_2841854

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
DIL-2 Antibody detects endogenous levels of total DIL-2.
RRID:
AB_2841854
Cite Format: Affinity Biosciences Cat# DF8650, RRID:AB_2841854.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C20ORF1; C20orf2; Chromosome 20 Open Reading Frame 1; Differentially expressed in cancerous and non-cancerous lung cells 2; Differentially expressed in cancerous and noncancerous lung cells 2; Differentially expressed in lung cells 2; Differentially expressed in lung cells; DIL 2; DIL-2; DIL2; FLS353; GD:C20orf1; HCA 519; HCA519; HCTP 4; HCTP4; Hepatocellular carcinoma associated antigen 519; Hepatocellular carcinoma-associated antigen 519; p100; Preferentially expressed in colorectal cancer; Protein fls353; REPP 86; repp86; Restricted expression proliferation associated protein 100; Restricted expression proliferation-associated protein 100; Targeting protein for Xklp2; TPX 2; TPX2; TPX2 microtubule associated homolog; TPX2 microtubule associated protein homolog; TPX2_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q9ULW0(TPX2_HUMAN) >>Visit HPA database.

Gene(ID):
TPX2(22974)
Expression:
Q9ULW0 TPX2_HUMAN:

Expressed in lung carcinoma cell lines but not in normal lung tissues.

Sequence:
MSQVKSSYSYDAPSDFINFSSLDDEGDTQNIDSWFEEKANLENKLLGKNGTGGLFQGKTPLRKANLQQAIVTPLKPVDNTYYKEAEKENLVEQSIPSNACSSLEVEAAISRKTPAQPQRRSLRLSAQKDLEQKEKHHVKMKAKRCATPVIIDEILPSKKMKVSNNKKKPEEEGSAHQDTAEKNASSPEKAKGRHTVPCMPPAKQKFLKSTEEQELEKSMKMQQEVVEMRKKNEEFKKLALAGIGQPVKKSVSQVTKSVDFHFRTDERIKQHPKNQEEYKEVNFTSELRKHPSSPARVTKGCTIVKPFNLSQGKKRTFDETVSTYVPLAQQVEDFHKRTPNRYHLRSKKDDINLLPSKSSVTKICRDPQTPVLQTKHRARAVTCKSTAELEAEELEKLQQYKFKARELDPRILEGGPILPKKPPVKPPTEPIGFDLEIEKRIQERESKKKTEDEHFEFHSRPCPTKILEDVVGVPEKKVLPITVPKSPAFALKNRIRMPTKEDEEEDEPVVIKAQPVPHYGVPFKPQIPEARTVEICPFSFDSRDKERQLQKEKKIKELQKGEVPKFKALPLPHFDTINLPEKKVKNVTQIEPFCLETDRRGALKAQTWKHQLEEELRQQKEAACFKARPNTVISQEPFVPKKEKKSVAEGLSGSLVQEPFQLATEKRAKERQELEKRMAEVEAQKAQQLEEARLQEEEQKKEELARLRRELVHKANPIRKYQGLEIKSSDQPLTVPVSPKFSTRFHC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Chicken
100
Rabbit
100
Xenopus
88
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9ULW0 As Substrate

Site PTM Type Enzyme
K44 Ubiquitination
K48 Sumoylation
K48 Ubiquitination
T51 Phosphorylation
K58 Acetylation
K58 Ubiquitination
T59 Phosphorylation
K63 Sumoylation
K63 Ubiquitination
T72 Phosphorylation P06493 (CDK1) , P24941 (CDK2)
K75 Acetylation
K75 Sumoylation
K75 Ubiquitination
Y81 Phosphorylation
Y82 Phosphorylation
S110 Phosphorylation
T113 Phosphorylation
S121 Phosphorylation O14965 (AURKA)
S125 Phosphorylation O14965 (AURKA)
K128 Acetylation
T147 Phosphorylation
S157 Phosphorylation
K158 Acetylation
S163 Phosphorylation
S174 Phosphorylation
T179 Phosphorylation
S185 Phosphorylation
S186 Phosphorylation
T195 Phosphorylation
C198 S-Nitrosylation
K203 Ubiquitination
K208 Sumoylation
S209 Phosphorylation
S218 Phosphorylation
K220 Sumoylation
K237 Sumoylation
S250 Phosphorylation
S252 Phosphorylation
K256 Sumoylation
S257 Phosphorylation
T264 Phosphorylation
Y278 Phosphorylation
K279 Sumoylation
K279 Ubiquitination
S285 Phosphorylation
R288 Methylation
S292 Phosphorylation
S293 Phosphorylation
K305 Acetylation
K305 Sumoylation
K305 Ubiquitination
S310 Phosphorylation
K313 Acetylation
K313 Ubiquitination
K314 Acetylation
T316 Phosphorylation
T320 Phosphorylation
S322 Phosphorylation
Y324 Phosphorylation
K336 Ubiquitination
T338 Phosphorylation
S346 Phosphorylation
K348 Ubiquitination
S356 Phosphorylation
K357 Ubiquitination
S358 Phosphorylation
S359 Phosphorylation
T361 Phosphorylation
T369 Phosphorylation P28482 (MAPK1)
T374 Phosphorylation
K375 Acetylation
K375 Ubiquitination
T382 Phosphorylation
K384 Sumoylation
T386 Phosphorylation
K396 Ubiquitination
K401 Sumoylation
K420 Ubiquitination
K421 Ubiquitination
K465 Ubiquitination
K476 Acetylation
K476 Sumoylation
K477 Sumoylation
T482 Phosphorylation
K485 Ubiquitination
S486 Phosphorylation
K492 Sumoylation
K492 Ubiquitination
T499 Phosphorylation
K500 Acetylation
K500 Sumoylation
K512 Ubiquitination
Y519 Phosphorylation
K524 Acetylation
K524 Ubiquitination
S539 Phosphorylation
S542 Phosphorylation
K560 Sumoylation
K565 Sumoylation
K567 Sumoylation
K567 Ubiquitination
K582 Acetylation
K585 Sumoylation
K585 Ubiquitination
K604 Methylation
K604 Sumoylation
K604 Ubiquitination
K609 Ubiquitination
R617 Methylation
K620 Ubiquitination
S634 Phosphorylation
K641 Sumoylation
K641 Ubiquitination
K645 Sumoylation
K645 Ubiquitination
S646 Phosphorylation
S652 Phosphorylation
S654 Phosphorylation
K666 Sumoylation
K666 Ubiquitination
K685 Ubiquitination
K714 Ubiquitination
K720 Sumoylation
K720 Ubiquitination
Y721 Phosphorylation
K727 Sumoylation
K727 Ubiquitination
S728 Phosphorylation
T734 Phosphorylation
S738 Phosphorylation P06493 (CDK1)
K740 Sumoylation
K740 Ubiquitination
S742 Phosphorylation

Research Backgrounds

Function:

Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation. TPX2 is inactivated upon binding to importin-alpha. At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation.

Subcellular Location:

Nucleus. Cytoplasm>Cytoskeleton>Spindle. Cytoplasm>Cytoskeleton>Spindle pole.
Note: During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. Is released from the nucleus in apoptotic cells and is detected on apoptotic microtubules.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in lung carcinoma cell lines but not in normal lung tissues.

Subunit Structure:

Interacts with AURKA. Interacts with importin-alpha; leading to inactivate TPX2. Interacts with HNRNPU; this interaction recruits HNRNPU to spindle microtubules (MTs).

Family&Domains:

Belongs to the TPX2 family.

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