GAS6 Antibody - #DF8659

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Product: GAS6 Antibody
Catalog: DF8659
Description: Rabbit polyclonal antibody to GAS6
Application: WB IHC IF/ICC
Cited expt.: IHC
Reactivity: Human, Mouse
Mol.Wt.: 79 kDa,70kDa; 75kD(Calculated).
Uniprot: Q14393
RRID: AB_2841863

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Clonality:
Polyclonal
Specificity:
GAS6 Antibody detects endogenous levels of total GAS6.
RRID:
AB_2841863
Cite Format: Affinity Biosciences Cat# DF8659, RRID:AB_2841863.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AXL receptor tyrosine kinase ligand; AXL stimulatory factor; AXLLG; AXS F; AXSF; DKFZp666G247; FLJ34709; Gas 6; GAS-6; Gas6; GAS6_HUMAN; Growth arrest specific 6; Growth arrest-specific protein 6; Growth-arrest-specific protein 6; Growth-arrest-specific protein 6 precursor;

Immunogens

Immunogen:

A synthesized peptide derived from human GAS6, corresponding to a region within the internal amino acids.

Uniprot:

Q14393(GAS6_HUMAN) >>Visit HPA database.

Gene(ID):
GAS6(2621)
Expression:
Q14393 GAS6_HUMAN:

Plasma. Isoform 1 and isoform 2 are widely expressed, isoform 1 being expressed at higher levels than isoform 2 in most tissues. Isoform 2 is the predominant form in spleen.

Sequence:
MAPSLSPGPAALRRAPQLLLLLLAAECALAALLPAREATQFLRPRQRRAFQVFEEAKQGHLERECVEELCSREEAREVFENDPETDYFYPRYLDCINKYGSPYTKNSGFATCVQNLPDQCTPNPCDRKGTQACQDLMGNFFCLCKAGWGGRLCDKDVNECSQENGGCLQICHNKPGSFHCSCHSGFELSSDGRTCQDIDECADSEACGEARCKNLPGSYSCLCDEGFAYSSQEKACRDVDECLQGRCEQVCVNSPGSYTCHCDGRGGLKLSQDMDTCEDILPCVPFSVAKSVKSLYLGRMFSGTPVIRLRFKRLQPTRLVAEFDFRTFDPEGILLFAGGHQDSTWIVLALRAGRLELQLRYNGVGRVTSSGPVINHGMWQTISVEELARNLVIKVNRDAVMKIAVAGDLFQPERGLYHLNLTVGGIPFHEKDLVQPINPRLDGCMRSWNWLNGEDTTIQETVKVNTRMQCFSVTERGSFYPGSGFAFYSLDYMRTPLDVGTESTWEVEVVAHIRPAADTGVLFALWAPDLRAVPLSVALVDYHSTKKLKKQLVVLAVEHTALALMEIKVCDGQEHVVTVSLRDGEATLEVDGTRGQSEVSAAQLQERLAVLERHLRSPVLTFAGGLPDVPVTSAPVTAFYRGCMTLEVNRRLLDLDEAAYKHSDITAHSCPPVEPAAA

Research Backgrounds

Function:

Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses.

(Microbial infection) Can bridge virus envelope phosphatidylserine to the TAM receptor tyrosine kinase Axl to mediate viral entry by apoptotic mimicry. Plays a role in Dengue cell entry by apoptotic mimicry. Plays a role in Vaccinia virus cell entry by apoptotic mimicry. Plays a role in ebolavirus and marburgvirus cell entry by apoptotic mimicry.

PTMs:

Proteolytically processed after secretion to yield a N-terminal 36 kDa protein and a C-terminal 50 kDa protein including the laminin G-like domains which activates AXL.

Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Plasma. Isoform 1 and isoform 2 are widely expressed, isoform 1 being expressed at higher levels than isoform 2 in most tissues. Isoform 2 is the predominant form in spleen.

Research Fields

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

References

1). Tumor-initiating cells escape tumor immunity via CCL8 from tumor-associated macrophages in mice. The Journal of clinical investigation, 2025 (PubMed: 39774471) [IF=13.3]

Application: IHC    Species: human    Sample:

Figure 4 GAS6 is highly expressed and plays an important role in TIC maintenance in ICC. (A) Circle plots show interaction strength and number of interactions in cell-cell communication among 6 major clusters (left) using CellChat and between epithelial and other cells (right). Line width correlates with communication probability. (B) Dot plots depict the significance (–log10 P value) and strength (log2 mean value) of detailed ligand-receptor pairs between epithelial and other cell types analyzed by CellphoneDB. (C) Violin plot displays the Gas6 expression differences between WNThi and WNTlo epithelial cell subclusters. (D) Violin plots show Axl and Mertk expression in MoMϕDC subclusters. (E–H) Flow plots (E) show Tom+GAS6+ tumor-infiltrating cells and the levels of MRC1, MERTK, and AXL expression in infiltrating Reg-TAMs. Graphs depict statistical analysis of GAS6+ in Tom+/– cells in mouse ICC (F) as well as AXL+ (G) and MERTK+ (H) expression in MRC1+/– cells in ICC. (I) Treatment strategy with anti-GAS6/IgG in ICC mice. (J) Representative liver morphology image for survival outcome analysis after anti-GAS6 treatment. (K) Kaplan-Meier OS curve for ICC mice after anti-GAS6 treatment. (L) Representative liver morphology image after 6 weeks of anti-GAS6 treatment (left) and statistical analysis of liver-to-body weight ratio (right). (M) Fluorescence images of lineage tracing (left) (scale bar: 50 μm). Statistical analysis of Tom+ cell numbers (middle), and Tom+ cell area (right) after treatment. (N–S) Representative images of GAS6 staining in human ICC tissue from SYSUCC cohort 1 (N) (scale bar: 200 μm) and FAHSYSU cohort 2 (Q) (scale bar: 100 μm). Kaplan-Meier curves based on GAS6 expression in ICC: OS and DFS for SYSUCC cohort 1 (O and P), and OS and RFS for FAHSYSU cohort 2 (R and S). Data represent the mean ± SD (F–H, L, and M). P value were calculated by 2-tailed,, unpaired Student’s t test (C, F–H, L, and M), log-rank test (K, O, P, R, and S).

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