LEG4 Antibody - #DF8674

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Product: LEG4 Antibody
Catalog: DF8674
Description: Rabbit polyclonal antibody to LEG4
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Rabbit
Mol.Wt.: 35 kDa; 36kD(Calculated).
Uniprot: P56470
RRID: AB_2841878

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(88%), Horse(88%), Rabbit(88%)
Clonality:
Polyclonal
Specificity:
LEG4 Antibody detects endogenous levels of total LEG4.
RRID:
AB_2841878
Cite Format: Affinity Biosciences Cat# DF8674, RRID:AB_2841878.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Antigen NY CO 27; Antigen NY-CO-27; Antigen NYCO27; GAL 4; Gal-4; GAL4; Galectin 4; Galectin-4; Galectin4; Homo sapiens galectin4 mRNA complete cds; L 36 lactose binding protein; L-36 lactose-binding protein; L36 lactose binding protein; L36LBP; Lactose binding lectin 4; Lactose-binding lectin 4; Lectin galactoside binding soluble 4; LEG4_HUMAN; LGALS4;

Immunogens

Immunogen:
Uniprot:

P56470(LEG4_HUMAN) >>Visit HPA database.

Gene(ID):
LGALS4(3960)
Sequence:
MAYVPAPGYQPTYNPTLPYYQPIPGGLNVGMSVYIQGVASEHMKRFFVNFVVGQDPGSDVAFHFNPRFDGWDKVVFNTLQGGKWGSEERKRSMPFKKGAAFELVFIVLAEHYKVVVNGNPFYEYGHRLPLQMVTHLQVDGDLQLQSINFIGGQPLRPQGPPMMPPYPGPGHCHQQLNSLPTMEGPPTFNPPVPYFGRLQGGLTARRTIIIKGYVPPTGKSFAINFKVGSSGDIALHINPRMGNGTVVRNSLLNGSWGSEEKKITHNPFGPGQFFDLSIRCGLDRFKVYANGQHLFDFAHRLSAFQRVDTLEIQGDVTLSYVQI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
88
Horse
88
Rabbit
88
Bovine
75
Sheep
75
Dog
75
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P56470 As Substrate

Site PTM Type Enzyme
T78 Phosphorylation
T207 Phosphorylation
S220 Phosphorylation
K261 Acetylation
Y288 Phosphorylation
S302 Phosphorylation

Research Backgrounds

Function:

Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions.

Subunit Structure:

Monomer.

Family&Domains:

Contains two homologous but distinct carbohydrate-binding domains.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.