MIC1 Antibody | Affinity Biosciences

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MIC1 Antibody - Western blot analysis of extracts from HepG2 cells, using MIC1 Antibody.

Product:	MIC1 Antibody
Catalog:	DF8677
Description:	Rabbit polyclonal antibody to MIC1
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Mol.Wt.:	74 kDa; 75kD(Calculated).
Uniprot:	Q96DM3
RRID:	AB_2841881

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

MIC1 Antibody detects endogenous levels of total MIC1.

RRID:

AB_2841881
Cite Format: Affinity Biosciences Cat# DF8677, RRID:AB_2841881.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

C18orf8; Chromosome 18 open reading frame 8; Colon cancer associated protein Mic1; Colon cancer-associated protein Mic1; HsT2591; Mic-1; MIC1; MIC1_HUMAN; Uncharacterized protein C18orf8;

Immunogens

Immunogen:

Uniprot:

Q96DM3(RMC1_HUMAN) >>Visit HPA database.

Gene(ID):

C18orf8(29919)

Sequence:

Q96DM3 RMC1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGEEDYYLELCERPVQFEKANPVNCVFFDEANKQVFAVRSGGATGVVVKGPDDRNPISFRMDDKGEVKCIKFSLENKILAVQRTSKTVDFCNFIPDNSQLEYTQECKTKNANILGFCWTSSTEIVFITDQGIEFYQVLPEKRSLKLLKSHNLNVNWYMYCPESAVILLSTTVLENVLQPFHFRAGTMSKLPKFEIELPAAPKSTKPSLSERDIAMATIYGQLYVLFLRHHSRTSNSTGAEVVLYHLPREGACKKMHILKLNRTGKFALNVVDNLVVVHHQDTETSVIFDIKLRGEFDGSVTFHHPVLPARSIQPYQIPITGPAAVTSQSPVPCKLYSSSWIVFQPDIIISASQGYLWNLQVKLEPIVNLLPDKGRLMDFLLQRKECKMVILSVCSQMLSESDRASLPVIATVFDKLNHEYKKYLDAEQSYAMAVEAGQSRSSPLLKRPVRTQAVLDQSDVYTHVLSAFVEKKEMPHKFVIAVLMEYIRSLNQFQIAVQHYLHELVIKTLVQHNLFYMLHQFLQYHVLSDSKPLACLLLSLESFYPPAHQLSLDMLKRLSTANDEIVEVLLSKHQVLAALRFIRGIGGHDNISARKFLDAAKQTEDNMLFYTIFRFFEQRNQRLRGSPNFTPGEHCEEHVAFFKQIFGDQALMRPTTF

PTMs - Q96DM3 As Substrate

Q96DM3

Site	PTM Type	Source
K33	Ubiquitination	Uniprot
K77	Ubiquitination	Uniprot
K86	Ubiquitination	Uniprot
K107	Ubiquitination	Uniprot
T108	Phosphorylation	Uniprot
K189	Ubiquitination	Uniprot
K205	Ubiquitination	Uniprot
T233	Phosphorylation	Uniprot
S234	Phosphorylation	Uniprot
S329	Phosphorylation	Uniprot
K373	Ubiquitination	Uniprot
K384	Acetylation	Uniprot
K422	Ubiquitination	Uniprot
K446	Ubiquitination	Uniprot
S559	Phosphorylation	Uniprot
T560	Phosphorylation	Uniprot
S571	Phosphorylation	Uniprot
K572	Ubiquitination	Uniprot
K595	Ubiquitination	Uniprot
Y610	Phosphorylation	Uniprot

Research Backgrounds

Q96DM3_RMC1_HUMAN

Function:

Componement of the CCZ1-MON1 RAB7A guanine exchange factor (GEF). Acts as a positive regulator of CCZ1-MON1A/B function necessary for endosomal/autophagic flux and efficient RAB7A localization.

Subcellular Location:

Lysosome membrane. Late endosome membrane.

Subunit Structure:

Found in a complex with RMC1, CCZ1 MON1A and MON1B.

Family&Domains:

Belongs to the RMC1 family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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MIC1 Antibody - #DF8677