Product: NARF Antibody
Catalog: DF8679
Description: Rabbit polyclonal antibody to NARF
Application: WB
Reactivity: Human, Rat
Prediction: Pig, Zebrafish, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 51 kDa; 51kD(Calculated).
Uniprot: Q9UHQ1
RRID: AB_2841883

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(100%), Zebrafish(80%), Horse(100%), Sheep(100%), Rabbit(89%), Dog(100%), Chicken(80%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
NARF Antibody detects endogenous levels of total NARF.
RRID:
AB_2841883
Cite Format: Affinity Biosciences Cat# DF8679, RRID:AB_2841883.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DKFZp434G0420; FLJ10067; GC17P078009; IOP2; Iron only hydrogenase like protein 2; Nuclear prelamin A recognition factor; Prenyl dependent prelamin A binding protein;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9UHQ1 NARF_HUMAN:

Ubiquitous. Predominantly expressed in skeletal muscle, heart and brain.

Sequence:
MKCEHCTRKECSKKTKTDDQENVSADAPSPAQENGEKGEFHKLADAKIFLSDCLACDSCMTAEEGVQLSQQNAKDFFRVLNLNKKCDTSKHKVLVVSVCPQSLPYFAAKFNLSVTDASRRLCGFLKSLGVHYVFDTTIAADFSILESQKEFVRRYRQHSEEERTLPMLTSACPGWVRYAERVLGRPITAHLCTAKSPQQVMGSLVKDYFARQQNLSPEKIFHVIVAPCYDKKLEALQESLPPALHGSRGADCVLTSGEIAQIMEQGDLSVRDAAVDTLFGDLKEDKVTRHDGASSDGHLAHIFRHAAKELFNEDVEEVTYRALRNKDFQEVTLEKNGEVVLRFAAAYGFRNIQNMILKLKKGKFPFHFVEVLACAGGCLNGRGQAQTPDGHADKALLRQMEGIYADIPVRRPESSAHVQELYQEWLEGINSPKAREVLHTTYQSQERGTHSLDIKW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Xenopus
90
Rabbit
89
Zebrafish
80
Chicken
80
Bovine
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UHQ1 As Substrate

Site PTM Type Enzyme
S29 Phosphorylation
K37 Ubiquitination
K42 Ubiquitination
K84 Ubiquitination
S102 Phosphorylation
Y105 Phosphorylation
K195 Ubiquitination
S196 Phosphorylation
K206 Ubiquitination
K219 Ubiquitination
K231 Acetylation
K232 Ubiquitination
K283 Ubiquitination
K286 Ubiquitination
K308 Ubiquitination
K326 Ubiquitination
K335 Ubiquitination
K358 Acetylation
T387 Phosphorylation
K394 Ubiquitination
Y404 Phosphorylation
S431 Phosphorylation
S444 Phosphorylation
S451 Phosphorylation

Research Backgrounds

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous. Predominantly expressed in skeletal muscle, heart and brain.

Subunit Structure:

Interacts with LMNA and binds to the farnesylated C-terminal domain.

Family&Domains:

Belongs to the NARF family.

Restrictive clause

 

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For Research Use Only.
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