NOL8 Antibody - #DF8683

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Product: NOL8 Antibody
Catalog: DF8683
Description: Rabbit polyclonal antibody to NOL8
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 131 kDa; 132kD(Calculated).
Uniprot: Q76FK4
RRID: AB_2841887

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(82%), Sheep(82%), Dog(82%), Chicken(91%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
NOL8 Antibody detects endogenous levels of total NOL8.
RRID:
AB_2841887
Cite Format: Affinity Biosciences Cat# DF8683, RRID:AB_2841887.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C9orf34; NOL8; NOL8_HUMAN; NOP132; Nucleolar protein 8; Nucleolar protein Nop132;

Immunogens

Immunogen:
Uniprot:

Q76FK4(NOL8_HUMAN) >>Visit HPA database.

Gene(ID):
NOL8(55035)
Expression:
Q76FK4 NOL8_HUMAN:

Expressed in various diffuse-type gastric cancers. Detected at lower levels in skeletal muscle.

Sequence:
MKVNRETKRLYVGGLSQDISEADLQNQFSRFGEVSDVEIITRKDDQGNPQKVFAYINISVAEADLKKCMSVLNKTKWKGGTLQIQLAKESFLHRLAQEREAAKAKKEESTTGNANLLEKTGGVDFHMKAVPGTEVPGHKNWVVSKFGRVLPVLHLKNQHKRKIIKYDPSKYCHNLKKIGEDFSNTIPISSLTWELEGGNDPMSKKRRGEFSDFHGPPKKIIKVQKDESSTGSLAMSTRPRRVIERPPLTQQQAAQKRTCDSITPSKSSPVPVSDTQKLKNLPFKTSGLETAKKRNSISDDDTDSEDELRMMIAKEENLQRTTQPSINESESDPFEVVRDDFKSGVHKLHSLIGLGIKNRVSCHDSDDDIMRNDREYDSGDTDEIIAMKKNVAKVKNSTEFSQMEKSTKKTSFKNRENCELSDHCIKLQKRKSNVESALSHGLKSLNRKSPSHSSSSEDADSASELADSEGGEEYNAMMKNCLRVNLTLADLEQLAGSDLKVPNEDTKSDGPETTTQCKFDRGSKSPKTPTGLRRGRQCIRPAEIVASLLEGEENTCGKQKPKENNLKPKFQAFKGVGCLYEKESMKKSLKDSVASNNKDQNSMKHEDPSIISMEDGSPYVNGSLGEVTPCQHAKKANGPNYIQPQKRQTTFESQDRKAVSPSSSEKRSKNPISRPLEGKKSLSLSAKTHNIGFDKDSCHSTTKTEASQEERSDSSGLTSLKKSPKVSSKDTREIKTDFSLSISNSSDVSAKDKHAEDNEKRLAALEARQKAKEVQKKLVHNALANLDGHPEDKPTHIIFGSDSECETEETSTQEQSHPGEEWVKESMGKTSGKLFDSSDDDESDSEDDSNRFKIKPQFEGRAGQKLMDLQSHFGTDDRFRMDSRFLETDSEEEQEEVNEKKTAEEEELAEEKKKALNVVQSVLQINLSNSTNRGSVAAKKFKDIIHYDPTKQDHATYERKRDDKPKESKAKRKKKREEAEKLPEVSKEMYYNIAMDLKEIFQTTKYTSEKEEGTPWNEDCGKEKPEEIQDPAALTSDAEQPSGFTFSFFDSDTKDIKEETYRVETVKPGKIVWQEDPRLQDSSSEEEDVTEETDHRNSSPGEASLLEKETTRFFFFSKNDERLQGSDLFWRGVGSNMSRNSWEARTTNLRMDCRKKHKDAKRKMKPK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Chicken
91
Pig
82
Sheep
82
Dog
82
Xenopus
82
Horse
0
Bovine
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q76FK4 As Substrate

Site PTM Type Enzyme
S35 Phosphorylation
Y55 Phosphorylation
S59 Phosphorylation
K105 Acetylation
K128 Acetylation
T133 Phosphorylation
K139 Acetylation
K222 Sumoylation
K256 Acetylation
K256 Ubiquitination
T258 Phosphorylation
T263 Phosphorylation
S265 Phosphorylation
S267 Phosphorylation
S268 Phosphorylation
S273 Phosphorylation
S296 Phosphorylation
S298 Phosphorylation
T302 Phosphorylation
S304 Phosphorylation
S325 Phosphorylation
S329 Phosphorylation
S331 Phosphorylation
K342 Ubiquitination
S350 Phosphorylation
S361 Phosphorylation
S365 Phosphorylation
Y376 Phosphorylation
S378 Phosphorylation
T381 Phosphorylation
S397 Phosphorylation
S411 Phosphorylation
S421 Phosphorylation
S432 Phosphorylation
S497 Phosphorylation
S508 Phosphorylation
S523 Phosphorylation
S525 Phosphorylation
T528 Phosphorylation
K574 Methylation
S612 Phosphorylation
S617 Phosphorylation
T628 Phosphorylation
S653 Phosphorylation
K657 Acetylation
S660 Phosphorylation
K666 Acetylation
S673 Phosphorylation
S681 Phosphorylation
S697 Phosphorylation
K703 Sumoylation
S707 Phosphorylation
S714 Phosphorylation
S719 Phosphorylation
S723 Phosphorylation
S837 Phosphorylation
S838 Phosphorylation
S843 Phosphorylation
S845 Phosphorylation
K855 Ubiquitination
T888 Phosphorylation
S890 Phosphorylation
K964 Acetylation
K966 Acetylation
K969 Acetylation
T1014 Phosphorylation
T1035 Phosphorylation
S1036 Phosphorylation
K1057 Sumoylation
S1082 Phosphorylation
S1083 Phosphorylation
S1084 Phosphorylation
S1098 Phosphorylation
S1099 Phosphorylation
S1104 Phosphorylation
S1138 Phosphorylation

Research Backgrounds

Function:

Plays an essential role in the survival of diffuse-type gastric cancer cells. Acts as a nucleolar anchoring protein for DDX47. May be involved in regulation of gene expression at the post-transcriptional level or in ribosome biogenesis in cancer cells.

PTMs:

Phosphorylated.

Subcellular Location:

Nucleus>Nucleolus.
Note: Localizes in the nucleolar-organizing region during ribosome biogenesis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in various diffuse-type gastric cancers. Detected at lower levels in skeletal muscle.

Subunit Structure:

Interacts with the GTP form of RRAGA, RRAGC and RRAGD. Interacts with NIP7. Interacts with DDX18; the interaction is RNA-dependent. Interacts with DDX47; the interaction is RNA-dependent.

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