RIOK3 Antibody - #DF8715
| Product: | RIOK3 Antibody |
| Catalog: | DF8715 |
| Description: | Rabbit polyclonal antibody to RIOK3 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 59 kDa; 59kD(Calculated). |
| Uniprot: | O14730 |
| RRID: | AB_2841919 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF8715, RRID:AB_2841919.
Fold/Unfold
Homolog of the Aspergillus nidulans sudD gene product; RIO kinase 3 (yeast); RIO kinase 3; RIOK3; RIOK3_HUMAN; Serine/threonine protein kinase RIO3; Serine/threonine-protein kinase RIO3; SudD (suppressor of bimD6, Aspergillus nidulans) homolog; SUDD; sudD homolog; SudD suppressor of Aspergillus nidulans bimD6 homolog; SudD suppressor of bimD6 homolog;
Immunogens
- O14730 RIOK3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDLVGVASPEPGTAAAWGPSKCPWAIPQNTISCSLADVMSEQLAKELQLEEEAAVFPEVAVAEGPFITGENIDTSSDLMLAQMLQMEYDREYDAQLRREEKKFNGDSKVSISFENYRKVHPYEDSDSSEDEVDWQDTRDDPYRPAKPVPTPKKGFIGKGKDITTKHDEVVCGRKNTARMENFAPEFQVGDGIGMDLKLSNHVFNALKQHAYSEERRSARLHEKKEHSTAEKAVDPKTRLLMYKMVNSGMLETITGCISTGKESVVFHAYGGSMEDEKEDSKVIPTECAIKVFKTTLNEFKNRDKYIKDDFRFKDRFSKLNPRKIIRMWAEKEMHNLARMQRAGIPCPTVVLLKKHILVMSFIGHDQVPAPKLKEVKLNSEEMKEAYYQTLHLMRQLYHECTLVHADLSEYNMLWHAGKVWLIDVSQSVEPTHPHGLEFLFRDCRNVSQFFQKGGVKEALSERELFNAVSGLNITADNEADFLAEIEALEKMNEDHVQKNGRKAASFLKDDGDPPLLYDE
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O14730 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S8 | Phosphorylation | Uniprot | |
| K21 | Ubiquitination | Uniprot | |
| K101 | Ubiquitination | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| K108 | Ubiquitination | Uniprot | |
| S110 | Phosphorylation | Uniprot | |
| S112 | Phosphorylation | Uniprot | |
| Y116 | Phosphorylation | Uniprot | |
| Y122 | Phosphorylation | Uniprot | |
| S125 | Phosphorylation | Uniprot | |
| S127 | Phosphorylation | Uniprot | |
| S128 | Phosphorylation | Uniprot | |
| Y142 | Phosphorylation | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| T150 | Phosphorylation | Uniprot | |
| K152 | Ubiquitination | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| K165 | Ubiquitination | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| K231 | Ubiquitination | Uniprot | |
| K236 | Ubiquitination | Uniprot | |
| K281 | Ubiquitination | Uniprot | |
| K290 | Ubiquitination | Uniprot | |
| K293 | Ubiquitination | Uniprot | |
| K300 | Ubiquitination | Uniprot | |
| K304 | Ubiquitination | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| K318 | Ubiquitination | Uniprot | |
| K376 | Ubiquitination | Uniprot | |
| K452 | Ubiquitination | Uniprot | |
| K456 | Ubiquitination | Uniprot | |
| K502 | Ubiquitination | Uniprot | |
| S505 | Phosphorylation | Uniprot | |
| K508 | Ubiquitination | Uniprot | |
| Y517 | Phosphorylation | Uniprot |
Research Backgrounds
Involved in regulation of type I interferon (IFN)-dependent immune response which plays a critical role in the innate immune response against DNA and RNA viruses. May act as an adapter protein essential for the recruitment of TBK1 to IRF3. Phosphorylates IFIH1 on 'Ser-828' interfering with IFIH1 filament assembly on long dsRNA and resulting in attenuated IFIH1-signaling. Can inhibit CASP10 isoform 7-mediated activation of the NF-kappaB signaling pathway. May play a role in the biogenesis of the 40S ribosomal subunit. Involved in the processing of 21S pre-rRNA to the mature 18S rRNA.
Autophosphorylated (in vitro).
Cytoplasm.
Widely expressed.
Interacts with CASP10. Interacts with IRF3; RIOK3 probybly mediates the interaction of TBK1 with IRF3. Associated with 40S pre-ribosomal particles.
Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.
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